본문 바로가기
KERD
논문KCI 등재

Apoptotic Activity of Curcumin and EF-24 in HTB-41 Human Salivary Gland Epidermoid Carcinoma Cells

김도경|이슬아|김정선|김춘성|김수관|박병선|유선경|오지수|박종태|고대산|김지원
대한구강생물학회 (Korean Academy Of Oral Biology)|2015.06|International Journal of Oral Biology|제40권 제2호|pp. 63-69|DOI http://dx.doi.org/10.11620/IJOB.2015.40.2.063

2

조회

0

다운로드

3

인용

0

참고문헌

키워드

apoptosiscurcumincell deathEF-24salivary gland epidermoid carcinoma cells
주제분류치의학

초록

Curcumin (diferuloylmethane), a constituent of turmeric powder derived from the rhizome of Curcuma longa, has been shown to inhibit the growth of various types of cancer cells by regulating cell proliferation and apoptosis. However, a need exists to design more effective analogs because of curcumin's poor intestinal absorption. EF-24 (diphenyl difluoroketone), the monoketone analog of curcumin, has shown good efficacy in anticancer screens. However, the effects of curcumin and EF-24 on salivary gland epidermoid carcinoma cells are not clearly established. The main goal of this study was to investigate the effects of curcumin and EF-24 on cell growth and induction of apoptosis in human salivary gland epidermoid carcinoma cells. Our studies showed that curcumin and EF-24 inhibited the growth of HTB-41 cells in a dose- and time-dependent manner, and the potency of EF-24 was > 34-fold that of curcumin. Treatment with curcumin or EF-24 resulted in nuclear condensation and fragmentation in HTB-41 cells, whereas the control HTB-41 cell nuclei retained their normal regular and oval shape. Curcumin and EF-24 promoted proteolytic cleavages of procaspase-3/-7/-9, resulting in an increase in the amount of cleaved caspase-3/-7/-9 in the HTB-41 cells. Caspase-3 and -7 activities were detected in viable HTB-41 cells treated with curcumin or EF-24. These results suggest that the curcumin and EF-24 inhibit cell proliferation and induce apoptosis in HTB-41 human salivary gland epidermoid carcinoma cells, and that they may have potential properties as an anti-cancer drug therapy.

Abstract

Curcumin (diferuloylmethane), a constituent of turmeric powder derived from the rhizome of Curcuma longa, has been shown to inhibit the growth of various types of cancer cells by regulating cell proliferation and apoptosis. However, a need exists to design more effective analogs because of curcumin's poor intestinal absorption. EF-24 (diphenyl difluoroketone), the monoketone analog of curcumin, has shown good efficacy in anticancer screens. However, the effects of curcumin and EF-24 on salivary gland epidermoid carcinoma cells are not clearly established. The main goal of this study was to investigate the effects of curcumin and EF-24 on cell growth and induction of apoptosis in human salivary gland epidermoid carcinoma cells. Our studies showed that curcumin and EF-24 inhibited the growth of HTB-41 cells in a dose- and time-dependent manner, and the potency of EF-24 was > 34-fold that of curcumin. Treatment with curcumin or EF-24 resulted in nuclear condensation and fragmentation in HTB-41 cells, whereas the control HTB-41 cell nuclei retained their normal regular and oval shape. Curcumin and EF-24 promoted proteolytic cleavages of procaspase-3/-7/-9, resulting in an increase in the amount of cleaved caspase-3/-7/-9 in the HTB-41 cells. Caspase-3 and -7 activities were detected in viable HTB-41 cells treated with curcumin or EF-24. These results suggest that the curcumin and EF-24 inhibit cell proliferation and induce apoptosis in HTB-41 human salivary gland epidermoid carcinoma cells, and that they may have potential properties as an anti-cancer drug therapy.

참고문헌 (40)

  1. PM Speight, "Salivary gland tumours", Oral Diseases, 8(5), pp.229~240 (2002), Wiley-Blackwell[DOI]
  2. Ellis GL, "Primary squamous cell carcinoma", pp.369~378 (1991), W. B. Saunders Company
  3. Yeung-Leung Cheng, "Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo", Cancer Letters, 222(2), pp.183~193 (2005), Elsevier BV[DOI]
  4. Dong Il Park, "Induction of apoptosis and inhibition of telomerase activity by aqueous extract from Platycodon grandiflorum in human lung carcinoma cells", Pharmacological Research, 51(5), pp.437~443 (2005), Elsevier BV[DOI]
  5. M.L. Tan, "Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line", Journal of Ethnopharmacology, 96(1-2), pp.287~294 (2005), Elsevier BV[DOI]
  6. Hoshino T, "Flow cytometric measurement of NK cell cytotoxicity", J Clin Lab Immunol, 36, pp.39~43 (1991)
  7. Ze Tian, "The cytotoxicity of naturally occurring styryl lactones", Phytomedicine, 13(3), pp.181~186 (2006), Elsevier BV[DOI]
  8. Smets LA, "Programmed cell death(apoptosis)and response to anti-cancer drugs", Anti-Cancer Drug, 5, pp.3~9 (1994)
  9. Adrian G Paschka, "Induction of apoptosis in prostate cancer cell lines by the green tea component, (−)-epigallocatechin-3-gallate", Cancer Letters, 130(1-2), pp.1~7 (1998), Elsevier BV[DOI]
  10. Green DR, "Mitochondria and Apoptosis", Science, 281(5381), pp.1309~1312 (1998), American Association for the Advancement of Science (AAAS)[DOI]
  11. Michael O. Hengartner, "The biochemistry of apoptosis", Nature, 407(6805), pp.770~776 (2000), Springer Nature[DOI]
  12. Scott H. Kaufmann, "Programmed cell death: alive and well in the new millennium", Trends in Cell Biology, 11(12), pp.526~534 (2001), Elsevier BV[DOI]
  13. Scott H. Kaufmann, "Induction of Apoptosis by Cancer Chemotherapy", Experimental Cell Research, 256(1), pp.42~49 (2000), Elsevier BV[DOI]
  14. John C Reed, "Apoptosis-regulating proteins as targets for drug discovery", Trends in Molecular Medicine, 7(7), pp.314~319 (2001), Elsevier BV[DOI]
  15. Mukhopadhyay A, "Curcumin downregulates cell survival mechanisms in human prostate cancer cell lines", Oncogene, 20, pp.7597~7609 (2001)
  16. J Miquel, "The curcuma antioxidants: pharmacological effects and prospects for future clinical use. A review", Archives of Gerontology and Geriatrics, 34(1), pp.37~46 (2002), Elsevier BV[DOI]
  17. Banerjee M, "Modulation of inflammatory mediators by ibuprofen and curcumin treatment during chronic inflammation in rat", Immunopharmacol Immunotoxicol, 25, pp.213~224 (2003)
  18. R P Sahu, "Activation of ATM", Chk1 by curcumin causes cell cycle arrest and apoptosis in human pancreatic cancer cells, 2009, pp.100(9), 1425~1433[DOI]
  19. Bharat B. Aggarwal, "RETRACTED: Curcumin induces the degradation of cyclin E expression through ubiquitin-dependent pathway and up-regulates cyclin-dependent kinase inhibitors p21 and p27 in multiple human tumor cell lines", Biochemical Pharmacology, 73(7), pp.1024~1032 (2007), Elsevier BV[DOI]
  20. Beatrice Bachmeier, "The Chemopreventive Polyphenol Curcumin Prevents Hematogenous Breast Cancer Metastases in Immunodeficient Mice", Cellular Physiology and Biochemistry, 19(1-4), pp.137~152 (2007), S. Karger AG[DOI]
  21. Hauser PJ, "Sensitivity of bladder cancer cells to curcumin and its derivatives depends on the extracellular matrix", Anticancer Res, 27, pp.737~740 (2007)
  22. Sharmila Shankar, "Involvement of Bcl-2 family members, phosphatidylinositol 3'-kinase", AKT and mitochondrial p53 in curcumin (diferulolylmethane)-induced apoptosis in prostate cancer, 2007, ~[DOI]
  23. H WAHL, "Curcumin enhances Apo2L", TRAIL-induced apoptosis in chemoresistant ovarian cancer cells, 2007, pp.105(1), 104~112[DOI]
  24. Hermann Ammon, "Pharmacol Curcuma longa", Planta Medica, 57(01), pp.1~7 (1991), Thieme Publishing Group[DOI]
  25. Ajay Goel, "Curcumin as “Curecumin”: From kitchen to clinic", Biochemical Pharmacology, 75(4), pp.787~809 (2008), Elsevier BV[DOI]
  26. Preetha Anand, "Bioavailability of Curcumin: Problems and Promises", Molecular Pharmaceutics, 4(6), pp.807~818 (2007), American Chemical Society (ACS)[DOI]
  27. Brian K. Adams, "Synthesis and biological evaluation of novel curcumin analogs as anti-cancer and anti-angiogenesis agents", Bioorganic & Medicinal Chemistry, 12(14), pp.3871~3883 (2004), Elsevier BV[DOI]
  28. Adams BK, "EF24, a novel synthetic curcumin analog, induces apoptosis in cancer cells via a redoxdependent mechanism", Anticancer Drugs, 16, pp.263~275 (2005)
  29. Myoung-Hwa Lee, "Ethanol Extracts of Angelica decursiva Induces Apoptosis in Human Oral Cancer Cells", International Journal of Oral Biology, 35(4), pp.215~220 (2010), 대한구강생물학회
  30. 신우철, "Growth Inhibition of Human Head and Neck Squamous Cell Carcinomas by Angelica decursiva Extracts", International Journal of Oral Biology, 35(4), pp.153~158 (2010), 대한구강생물학회
  31. Ji Young Kim, "Antioxidant Potential of Dimethyl Lithospermate Isolated from Salvia miltiorrhiza (Red Sage) Against Peroxynitrite", Journal of Medicinal Food, 11(1), pp.21~28 (2008), 한국식품영양과학회[DOI]
  32. Chun Sung Kim, "Inhibition of L-Type Amino Acid Transporter Modulates the Expression of Cell Cycle Regulatory Factors in KB Oral Cancer Cells", Biological & Pharmaceutical Bulletin, 33(7), pp.1117~1121 (2010), Pharmaceutical Society of Japan[DOI]
  33. Cohen GM, "Caspases : the executioners of apoptosis", Biochem J, 326, pp.1~16 (1997)
  34. R. Datta, "Caspase-3-mediated Cleavage of Protein Kinase C   in Induction of Apoptosis", Journal of Biological Chemistry, 272(33), pp.20317~20320 (1997), American Society for Biochemistry & Molecular Biology (ASBMB)[DOI]
  35. Xuesong Liu, "DFF, a Heterodimeric Protein That Functions Downstream of Caspase-3 to Trigger DNA Fragmentation during Apoptosis", Cell, 89(2), pp.175~184 (1997), Elsevier BV[DOI]
  36. Wei Hu, "Anticancer therapy targeting the apoptotic pathway", The Lancet Oncology, 4(12), pp.721~729 (2003), Elsevier BV[DOI]
  37. Leonidas Christou, "Treatment of plasma cell leukemia with vincristine, liposomal doxorubicin and dexamethasone", European Journal of Haematology, 67(1), pp.51~53 (2001), Wiley-Blackwell[DOI]
  38. Ashis Mukherjee, "Advances in Cancer Therapy with Plant Based Natural Products", Current Medicinal Chemistry, 8(12), pp.1467~1486 (2001), Bentham Science Publishers Ltd.[DOI]
  39. John M. Pezzuto, "Plant-derived anticancer agents", Biochemical Pharmacology, 53(2), pp.121~133 (1997), Elsevier BV[DOI]
  40. Geert van Poppel, "Vitamins and cancer", Cancer Letters, 114(1-2), pp.195~202 (1997), Elsevier BV[DOI]

관련 논문AI추천

Mechanism Underlying Curcumin-induced Apoptosis and Cell Cycle Arrest on SCC25 Human Tongue Squamous Cell Carcinoma Cell Line곽현호, 김규천, 김인령, 문정본, 박봉수, 이기현대한구강생물학회 · International Journal of Oral Biology2014 · 제39권 제1호Apoptotic Effect of Co-treatment with Curcumin and Cisplatin on SCC25 Human Tongue Squamous Cell Carcinoma Cell Line곽현호, 김규천, 김용호, 김인령, 박봉수, 손현진대한구강생물학회 · International Journal of Oral Biology2014 · 제39권 제3호Apoptotic activity of demethoxycurcumin in MG-63 human osteosarcoma cells강경록, 김도경, 김재성, 김춘성, 김태현, 김흥중, 박종현, 서정연, 유선경, 전홍성대한구강생물학회 · International Journal of Oral Biology2021 · 제46권 제1호Resveratrol induces the apoptosis associated with activation of caspases in HTB-41 human salivary gland epidermoid carcinoma cells고대선, 김도경, 김수관, 김춘성, 박병선, 임경섭치의학연구원 · Oral Biology Research2016 · 제40권 제1호Anti-proliferative and Anti-telomerase Activity of Curcuma Rhizome Extract on Oral Squamous Cell Carcinoma and Osteosarcoma Cells김경진, 김정희대한구강생물학회 · International Journal of Oral Biology2007 · 제32권 제4호Antiproliferative Effects of Curcumin Analogues배현옥, Bon Soon Koo, Hun-Taeg Chung, Ki-nam Lee, Myong-Soo Chong, Seon-Choong Jeong대한예방한의학회 · 대한예방한의학회지2007 · 제11권 제1호

이 간행물의 다른 논문 (5건)

Botulinum Toxin Type A Attenuates Activation of Glial Cells in Rat Medullary Dorsal Horn with CFA-induced Inflammatory Pain박민경, 김혜진, 이민경, 조진호, 김민지, 안동국, 양귀예, 주진숙pp. 71-77Antimicrobial Effect of Coptidis rhizome Extract against Mutans Streptococci and Periodontopathogens임윤경, 국중기, 박순낭pp. 79-83Autophagy Inhibition Promotes Quercetin Induced Apoptosis in MG-63 Human Osteosarcoma cells유수빈, 김인령, 김용호, 박봉수, 박혜련, 박성진pp. 85-91Clinical and Microbiological Study about Efficacy of Air-polishing and Scaling and Root-planing박도영, 김병옥, 유상준, 양건일pp. 93-101Detection of Mitochondrial Reactive Oxygen Species in Living Rat Trigeminal Caudal Neurons천상우, 이해인pp. 103-109
원문 보기