본문 바로가기
KERD
논문KCI 등재

Mechanism Underlying Curcumin-induced Apoptosis and Cell Cycle Arrest on SCC25 Human Tongue Squamous Cell Carcinoma Cell Line

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류치의학

초록

Several studies have shown that curcumin, which is derivedfrom the rhizomes of turmeric, possesses antimicrobial,antioxidant and anti-inflammatory properties. The antitumorproperties of curcumin have also now been demonstratedmore recently in different cancers. This study was undertakento investigate the modulation of cell cycle-related proteins andthe mechanisms underlying apoptosis induction by curcuminin the SCC25 human tongue squamous cell carcinoma cellline. Curcumin treatment of the SCC25 cells resulted in a timeanddose-dependent reduction in cell viability and cell growth,and onset of apoptotic cell death. The curcumin-treatedSCC25 cells showed several types of apoptotic manifestations,such as nuclear condensation, DNA fragmentation, reducedMMP and proteasome activity, and a decreased DNA content. In addition, the treated SCC25 cells showed a release ofcytochrome c into the cytosol, translocation of AIF andDFF40/CAD into the nuclei, a significant shift in theBax/Bcl-2 ratio, and the activation of caspase-9, caspase-7,caspase-6, caspase-3, PARP, lamin A/C, and DFF45/ICAD. Furthermore, curcumin exposure resulted in a downregulation of G1 cell cycle-related proteins and upregulation of p27KIP1. Taken together, our findings demonstrate that curcuminstrongly inhibits cell proliferation by modulating theexpression of G1 cell cycle-related proteins and inducingapoptosis via proteasomal, mitochondrial, and caspasecascades in SCC25 cells.

Abstract

Several studies have shown that curcumin, which is derivedfrom the rhizomes of turmeric, possesses antimicrobial,antioxidant and anti-inflammatory properties. The antitumorproperties of curcumin have also now been demonstratedmore recently in different cancers. This study was undertakento investigate the modulation of cell cycle-related proteins andthe mechanisms underlying apoptosis induction by curcuminin the SCC25 human tongue squamous cell carcinoma cellline. Curcumin treatment of the SCC25 cells resulted in a timeanddose-dependent reduction in cell viability and cell growth,and onset of apoptotic cell death. The curcumin-treatedSCC25 cells showed several types of apoptotic manifestations,such as nuclear condensation, DNA fragmentation, reducedMMP and proteasome activity, and a decreased DNA content. In addition, the treated SCC25 cells showed a release ofcytochrome c into the cytosol, translocation of AIF andDFF40/CAD into the nuclei, a significant shift in theBax/Bcl-2 ratio, and the activation of caspase-9, caspase-7,caspase-6, caspase-3, PARP, lamin A/C, and DFF45/ICAD. Furthermore, curcumin exposure resulted in a downregulation of G1 cell cycle-related proteins and upregulation of p27KIP1. Taken together, our findings demonstrate that curcuminstrongly inhibits cell proliferation by modulating theexpression of G1 cell cycle-related proteins and inducingapoptosis via proteasomal, mitochondrial, and caspasecascades in SCC25 cells.

참고문헌 (65)

  1. Aggarwal S, "Inhibition of growth and survival of human head and neck squamous cell carcinoma cells by curcumin via modulation of nuclear factor-kappaB signaling", Int J Cancer, 111, pp.679~692 (2004)
  2. Fiorillo C, "Curcumin protects cardiac cells against ischemia-reperfusion injury : effects on oxidative stress, NF-kappaB, and JNK pathways", Free Radic Biol Med, 45, pp.839~846 (2008)
  3. Panicker SR, "Curcumin attenuates glucoseinduced monocyte chemoattractant protein-1 synthesis in aortic endothelial cells by modulating the nuclear factorkappaB pathway", Pharmacology, 85, pp.18~26 (2010)
  4. Chuang SE, "Basal levels and patterns of anticancer druginduced activation of nuclear factor-kappaB(NF-kappaB), and its attenuation by tamoxifen, dexamethasone, and curcumin in carcinoma cells", Biochem Pharmacol, 63, pp.1709~1716 (2002)
  5. Nakamura K, "Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines", Int J Oncol, 21, pp.825~830 (2002)
  6. Han SS, "Curcumin suppresses activation of NF-kappaB and AP-1 induced by phorbol ester in cultured human promyelocytic leukemia cells", J Biochem Mol Biol, 35, pp.337~342 (2002)
  7. Bachmeier BE, "Curcumin downregulates the inflammatory cytokines CXCL1 and-2 in breast cancer cells via NFkappaB", Carcinogenesis, 29, pp.779~789 (2008)
  8. Aravindan N, "Curcumin inhibits NFkappaB mediated radioprotection and modulate apoptosis related genes in human neuroblastoma cells", Cancer Biol Ther, 7, pp.569~576 (2008)
  9. Wang D, "Liposome-encapsulated curcumin suppresses growth of head and neck squamous cell carcinoma in vitro and in xenografts through the inhibition of nuclear factor kappaB by an AKTindependent pathway", Clin Cancer Res, 14, pp.6228~6236 (2008)
  10. Carson DA, "Apoptosis and disease", Lancet, 341, pp.1251~1254 (1993)
  11. Ohta K, "Apoptosis of eosinophils and lymphocytes in allergic inflammation", J Allergy Clin Immunol, 104, pp.14~21 (1999)
  12. Wyllie AH, "Cell death : the significance of apoptosis", Int Rev Cytol, 68, pp.251~306 (1980)
  13. Williams GT, "Programmed cell death : apoptosis and oncogenesis", Cell, 65, pp.1097~1098 (1991)
  14. Clayman GL, "Report of the Tenth International Symposium of the Foundation for Promotion of Cancer Research : Basic and Clinical Research in Head and Neck cancer", Jpn J Clin Oncol, 27, pp.361~368 (1997)
  15. Shen J, "Enhancement of cisplatin induced apoptosis by suberoylanilide hydroxamic acid in human oral squamous cell carcinoma cell lines", Biochem Pharmacol, 73, pp.1901~1909 (2007)
  16. Bell RB, "Tongue cancer: Is there a difference in survival compared with other subsites in the oral cavity?", J Oral Maxillofac Surg, 65, pp.229~236 (2007)
  17. Lo WL, "Outcomes of oral squamous cell carcinoma in Taiwan after surgical therapy : factors affecting survival", J Oral Maxillofac Surg, 61, pp.751~758 (2003)
  18. Shintani S, "Anti-tumor effect of radiation response by combined treatment with angiogenesis inhibitor, TNP-470, in oral squamous cell carcinoma", Oral Oncol, 42, pp.66~72 (2006)
  19. Prince A, "Head and neck squamous cell carcinoma : new translational therapies", Mt Sinai J Med, 77, pp.684~699 (2010)
  20. Zlotogorski A, "Nutraceuticals as new treatment approaches for oral cancer: II. Green tea extracts and resveratrol", Oral Oncol, 49, pp.502~506 (2013)
  21. Lee UL, "The chemopreventive properties and therapeutic modulation of green tea polyphenols in oral squamous cell carcinoma", ISRN Oncol, 2011, pp.403707~ (2011)
  22. Yen CY, "Antiproliferative effects of goniothalamin on Ca9-22 oral cancer cells through apoptosis, DNA damage and ROS induction", Mutat Res, 747, pp.253~258 (2012)
  23. Shih YH, "In vitro antimicrobial and anticancer potential of hinokitiol against oral pathogens and oral cancer cell lines", Microbiol Res, 168, pp.254~262 (2013)
  24. Yang CW, "Curcumin induces the apoptosis of human monocytic leukemia THP-1 cells via the activation of JNK", ERK pathways, 2012, pp.12, 22~
  25. Lee JY, "Curcumin induces EGFR degradation in lung adenocarcinoma and modulates p38 activation in intestine:the versatile adjuvant for gefitinib therapy", PLoS One, 6, pp.e23756~ (2011)
  26. Wu SH, "Curcumin induces apoptosis in human non-small cell lung cancer NCI-H460cells through ER stress and caspase cascade-and mitochondria-dependent pathways", Anticancer Res, 30, pp.2125~2133 (2010)
  27. Kim HM, "Effect of Syzygium aromaticum extract on immediate hypersensitivity in rats", J Ethnopharmacol, 60, pp.125~131 (1998)
  28. Kim HM, "Magnoliae flos inhibits mast celldependent immediate-type allergic reactions", Pharmacol Res, 39, pp.107~111 (1999)
  29. Seo N, "Anti-allergic Psidium guajava extracts exert an antitumor effect by inhibition of T regulatory cells and resultant augmentation of Th1 cells", Anticancer Res, 25, pp.3763~3770 (2005)
  30. Angelini A, "Modulation of multidrug resistance p-glycoprotein activity by flavonoids and honokiol in human doxorubicin-resistant sarcoma cells(MES-SA", DX-5) : implications for natural sedatives as chemosensitizing agents in cancer therapy, 2010, pp.24, 197~205
  31. Ravindran J, "Curcumin and cancer cells: how many ways can curry kill tumor cells selectively?", AAPS J, 11, pp.495~510 (2009)
  32. Wang Z, "Notch-1 down-regulation by curcumin is associated with the inhibition of cell growth and the induction of apoptosis in pancreatic cancer cells", Cancer, 106, pp.2503~2513 (2006)
  33. Sarkar FH, "Lesson learned from nature for the development of novel anti-cancer agents : implication of isoflavone, curcumin, and their synthetic analogs", Curr Pharm Des, 16, pp.1801~1812 (2010)
  34. Meng L, "Eponemycin exerts its antitumor effect through the inhibition of proteasome function", Cancer Res, 59, pp.2798~2801 (1999)
  35. Drexler HC, "Inhibition of proteasome function induces programmed cell death in proliferating endothelial cells", FASEB J, 14, pp.65~77 (2000)
  36. Grimm LM, "Proteasomes play an essential role in thymocyte apoptosis", EMBO J, 15, pp.3835~3844 (1996)
  37. Orlowski RZ, "The role of the ubiquitin-proteasome pathway in apoptosis", Cell Death Differ, 6, pp.303~313 (1999)
  38. Li B, "Bax degradation by the ubiquitin", proteasomedependent pathway : involvement in tumor survival and progression, 2000, pp.97, 3850~3855
  39. Kroemer G, "Mitochondrial control of cell death", Nat Med, 6, pp.513~519 (2000)
  40. Galluzzi L, "Mitochondrial gateways to cancer", Mol Aspects Med, 31, pp.1~20 (2010)
  41. Prabhu SB, "Role of apoptosisinducing factor(Aif)in the T cell lineage", Indian J Med Res, 138, pp.577~590 (2013)
  42. Boland ML, "Mitochondrial Dysfunction in Cancer", Front Oncol, 3, pp.292~ (2013)
  43. Braun RJ, "Mitochondrion-mediated cell death : dissecting yeast apoptosis for a better understanding of neurodegeneration", Front Oncol, 2, pp.182~ (2012)
  44. Orrenius S, "Mitochondrial regulation of apoptotic cell death", Toxicol Lett, 149, pp.19~23 (2004)
  45. Hengartner MO, "The biochemistry of apoptosis", Nature, 407, pp.770~776 (2000)
  46. Barczyk K, "Serum cytochrome c indicates in vivo apoptosis and can serve as a prognostic marker during cancer therapy", Int J Cancer, 116, pp.167~173 (2005)
  47. Tsujimoto Y, "Bcl-2 family : life-or-death switch", FEBS Lett, 466, pp.6~10 (2000)
  48. Kuwana T, "Bcl-2-family proteins and the role of mitochondria in apoptosis", Curr Opin Cell Biol, 15, pp.691~699 (2003)
  49. Wagenknecht B, "Proteasome inhibitor-induced apoptosis of glioma cells involves the processing of multiple caspases and cytochrome c release", J Neurochem, 75, pp.2288~2297 (2000)
  50. Marshansky V, "Proteasomes modulate balance among proapoptotic and antiapoptotic Bcl-2 family members and compromise functioning of the electron transport chain in leukemic cells", J Immunol, 166, pp.3130~3142 (2001)
  51. Zou H, "An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9", J Biol Chem, 274, pp.11549~11556 (1999)
  52. Daugas E, "Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis", FASEB J, 14, pp.729~739 (2000)
  53. Acehan D, "Three-dimensional structure of the apoptosome : implications for assembly, procaspase-9 binding, and activation", Mol Cell, 9, pp.423~432 (2002)
  54. Gross A, "BCL-2 family members and the mitochondria in apoptosis", Genes Dev, 13, pp.1899~1911 (1999)
  55. Porter AG, "Protein translocation in apoptosis", Trends Cell Biol, 9, pp.394~401 (1999)
  56. Cheng AC, "Induction of apoptosis by Uncaria tomentosa through reactive oxygen species production, cytochrome c release, and caspases activation in human leukemia cells", Food Chem Toxicol, 45, pp.2206~2218 (2007)
  57. Lee SC, "Functional proteomics of resveratrol-induced colon cancer cell apoptosis : caspase-6-mediated cleavage of lamin A is a major signaling loop", Proteomics, 6, pp.2386~2394 (2006)
  58. Ilmarinen-Salo P, "Nitric oxide induces apoptosis in GM-CSF-treated eosinophils via caspase-6-dependent lamin and DNA fragmentation", Pulm Pharmacol Ther, 23, pp.365~371 (2010)
  59. Liu JD, "Molecular mechanisms of G0", G1 cellcycle arrest and apoptosis induced by terfenadine in human cancer cells, 2003, pp.37, 39~50
  60. Zheng R, "Polycystin-1 induced apoptosis and cell cycle arrest in G0", G1 phase in cancer cells, 2008, pp.32, 427~435
  61. Pavletich NP, "Mechanisms of cyclin-dependent kinase regulation : structures of Cdks, their cyclin activators, and Cip and INK4 inhibitors", J Mol Biol, 287, pp.821~828 (1999)
  62. Polyak K, "Cloning of p27Kip1, a cyclindependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals", Cell, 78, pp.59~66 (1994)
  63. Coats S, "Requirement of p27Kip1 for restriction point control of the fibroblast cell cycle", Science, 272, pp.877~880 (1996)
  64. Colman MS, "Regulation of p53stability and activity in response to genotoxic stress", Mutat Res, 462, pp.179~188 (2000)
  65. "Bull Cancer", 1999, 345~357

관련 논문AI추천

Apoptotic Effect of Co-treatment with Curcumin and Cisplatin on SCC25 Human Tongue Squamous Cell Carcinoma Cell Line곽현호, 김규천, 김용호, 김인령, 박봉수, 손현진대한구강생물학회 · International Journal of Oral Biology2014 · 제39권 제3호Mechanism Underlying Shikonin-induced Apoptosis and Cell Cycle Arrest on SCC25 Human Tongue Squamous Cell Carcinoma Cell Line김용호, 김인령, 박봉수, 박성진, 오상헌대한구강생물학회 · International Journal of Oral Biology2015 · 제40권 제1호Apoptotic Activity of Curcumin and EF-24 in HTB-41 Human Salivary Gland Epidermoid Carcinoma Cells고대산, 김도경, 김수관, 김정선, 김지원, 김춘성, 박병선, 박종태, 오지수, 유선경, 이슬아대한구강생물학회 · International Journal of Oral Biology2015 · 제40권 제2호Mechanism underlying Chios gum mastic-induced apoptosis on SCC25 human tongue squamous cell carcinoma cell line곽현호, 김규천, 김인령, 박봉수, 신상훈, Chul-Hoon Kim, Seung-Eun Lee, Young-Joo Hur대한구강생물학회 · International Journal of Oral Biology2009 · 제34권 제2호Anti-proliferative and Anti-telomerase Activity of Curcuma Rhizome Extract on Oral Squamous Cell Carcinoma and Osteosarcoma Cells김경진, 김정희대한구강생물학회 · International Journal of Oral Biology2007 · 제32권 제4호Apoptotic activity of demethoxycurcumin in MG-63 human osteosarcoma cells강경록, 김도경, 김재성, 김춘성, 김태현, 김흥중, 박종현, 서정연, 유선경, 전홍성대한구강생물학회 · International Journal of Oral Biology2021 · 제46권 제1호

이 간행물의 다른 논문 (6건)