A Neuromedin B Receptor Blockade Inhibits the Growth of Human Oral Cancer Cells
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Neuromedin B (NMB) acts as a growth factor or amorphogen and plays a role in cancer progression. Indeed,the NMB receptor (NMB-R) is overexpressed in differenttypes of tumors. In our current study, we investigated theinvolvement of NMB-R in the proliferation of oral cancercells. Human oral squamous cell carcinoma (SCC) andhuman oral cancer cells, SCC-25 cells were found to beNMB-R-positive. The NMB-R antagonist PD168368inhibited the proliferation of SCC-25 cells and reduced theircolony formation capacity. We also found that PD168368induced the cell cycle arrest and apoptosis of SCC-25 cells ina dose-/time-dependent manner. Overall, this antitumoractivity of PD168368 in human oral cancer cells suggests thatNMB-R is a potential target for the future prevention andtreatment of human cancers.
Abstract
Neuromedin B (NMB) acts as a growth factor or amorphogen and plays a role in cancer progression. Indeed,the NMB receptor (NMB-R) is overexpressed in differenttypes of tumors. In our current study, we investigated theinvolvement of NMB-R in the proliferation of oral cancercells. Human oral squamous cell carcinoma (SCC) andhuman oral cancer cells, SCC-25 cells were found to beNMB-R-positive. The NMB-R antagonist PD168368inhibited the proliferation of SCC-25 cells and reduced theircolony formation capacity. We also found that PD168368induced the cell cycle arrest and apoptosis of SCC-25 cells ina dose-/time-dependent manner. Overall, this antitumoractivity of PD168368 in human oral cancer cells suggests thatNMB-R is a potential target for the future prevention andtreatment of human cancers.
참고문헌 (29)
- Gonzalez N, "Bombesin-related peptides and their receptors : recent advances in their role in physiology and disease states", Curr Opin Endocrinol Diabetes Obes, 15, pp.58~64 (2008)
- Ohki-Hamazaki H, "Development and function of bombesin-like peptides and their receptors", Int J Dev Biol, 49, pp.293~300 (2005)
- Patel O, "Gastrin-releasing peptide and cancer", Biochim Biophys Acta, 1766, pp.23~41 (2006)
- Preston SR, "Bombesin-like peptides and cancer", Crit Rev Oncol Hematol, 23, pp.225~238 (1996)
- Park HJ, "Neuromedin B induces angiogenesis via activation of ERK and Akt in endothelial cells", Exp Cell Res, 315, pp.3359~3369 (2009)
- Martinez A, "Gastrin-releasing peptide (GRP) induces angiogenesis and the specific GRP blocker 77427 inhibits tumor growth in vitro and in vivo", Oncogene, 24, pp.4106~4113 (2005)
- Zhou J, "Targeting gastrin-releasing peptide receptors for cancer treatment", Anticancer Drugs, 15, pp.921~927 (2004)
- Qiao J, "Gastrin-releasing peptide receptor silencing suppresses the tumorigenesis and metastatic potential of neuroblastoma", Proc Natl Acad Sci USA, 105, pp.12891~12896 (2008)
- Hohla F, "Growth inhibition of non-small-cell lung carcinoma by BN", GRP antagonist is linked with suppression of K-Ras, COX-2, and pAkt, 2007, pp.104, 18671~18676
- Park HJ, "Neuromedin B receptor antagonist suppresses tumor angiogenesis and tumor growth in vitro and in vivo", Cancer Lett, 312, pp.117~127 (2011)
- da Silva SD, "Advances and applications of oral cancer basic research", Oral Oncol, 47, pp.783~791 (2011)
- Brinkman BM, "Disease mechanism and biomarkers of oral squamous cell carcinoma", Curr Opin Oncol, 18, pp.228~233 (2006)
- 신우철, "Growth Inhibition of Human Head and Neck Squamous Cell Carcinomas by Angelica decursiva Extracts", International Journal of Oral Biology, 35(4), pp.153~158 (2010), 대한구강생물학회
- 장남미, "The Role of HS-1200 Induced Autophagy in Oral Cancer Cells", International Journal of Oral Biology, 38(3), pp.93~100 (2013), 대한구강생물학회
- Lizard G, "Flow cytometry measurement of DNA fragmentation in the course of cell death via apoptosis. New techniques for evaluation of DNA status for the pathologist", Ann Pathol, 17, pp.61~66 (1997)
- Rozengurt E, "Neuropeptides as growth factors for normal and cancerous cells", Trends Endocrinol Metab, 13, pp.128~134 (2002)
- Heasley LE, "Autocrine and paracrine signaling through neuropeptide receptors in human cancer", Oncogene, 20, pp.1563~1569 (2001)
- Jensen RT, "International Union of Pharmacology. LXVIII. Mammalian bombesin receptors: nomenclature, distribution, pharmacology, signaling, and functions in normal and disease states", Pharmacol Rev, 60, pp.1~42 (2008)
- Cardona C, "Production of neuromedin B and neuromedin B gene expression in human lung tumor cell lines", Cancer Res, 51, pp.5205~5211 (1991)
- Jensen JA, "The case for gastrinreleasing peptide acting as a morphogen when it and its receptor are aberrantly expressed in cancer", Peptides, 22, pp.689~699 (2001)
- Matusiak D, "Neuromedin B and its receptor are mitogens in both normal and malignant epithelial cells lining the colon", Am J Physiol Gastrointest Liver Physiol, 288, pp.G718~728 (2005)
- Egloff AM, "Elevated gastrin-releasing peptide receptor mRNA expression in buccal mucosa:association with head and neck squamous cell carcinoma", Head Neck, 35, pp.270~279 (2013)
- Lango MN, "Gastrin-releasing peptide receptor-mediated autocrine growth in squamous cell carcinoma of the head and neck", J Natl Cancer Inst, 94, pp.375~383 (2002)
- Majumdar ID, "Biology of mammalian bombesin-like peptides and their receptors", Curr Opin Endocrinol Diabetes Obes, 18, pp.68~74 (2011)
- Ohki-Hamazaki H, "Neuromedin B", Prog Neurobiol, 62, pp.297~312 (2000)
- Park HJ, "Hypoxia Regulates the Expression of the Neuromedin B Receptor through a Mechanism Dependent on Hypoxia-Inducible Factor-1α", PLoS One, 8, pp.e82868~ (2013)
- Moody TW, "Neuromedin B receptors regulate EGF receptor tyrosine phosphorylation in lung cancer cells", Eur J Pharmacol, 637, pp.38~45 (2010)
- Cornelio DB, "Gastrin-releasing peptide receptor as a molecular target in experimental anticancer therapy", Ann Oncol, 18, pp.1457~1466 (2007)
- Moody TW, "Nonpeptide neuromedin B receptor antagonists inhibit the proliferation of C6 cells", Eur J Pharmacol, 409, pp.133~142 (2000)