Effect of Sambucus sieboldiana Extract on the Cell Growth and Extracellular Matrix Formation in Osteoblast Cells
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Sambucus sieboldiana (SS) is a member of the familyCaprifoliaceae and has been recommended as a functionalmaterial because of its several bioactivities. Althoughnumerous literatures are available on the pharmacological andbiological activities, the biological activity of SS in boneregeneration process has not yet been well-defined. Therefore,in this study, the effect of SS was investigated in the proliferationand differentiation of MC3T3-E1 osteoblastic cell line. Thetreatment of SS did not significantly affect the cell proliferationin MC3T3-E1 cells. SS significantly accelerated themineralization and significantly increased the expression ofalkaline phosphatase (ALP) and osteocalcin (OC) mRNAs,compared to the control, in the differentiation of MC3T3-E1cells. SS significantly accelerated the decrease of osteonectin(ON) mRNA expression as compared with the control in atime-dependent manner in the differentiation of MC3T3-E1cells. These results suggest that the SS facilitate the osteoblastdifferentiation and mineralization in MC3T3-E1 osteoblastic cells. Therefore, there may be potential properties fordevelopment and clinical application of bone regenerationmaterials.
Abstract
Sambucus sieboldiana (SS) is a member of the familyCaprifoliaceae and has been recommended as a functionalmaterial because of its several bioactivities. Althoughnumerous literatures are available on the pharmacological andbiological activities, the biological activity of SS in boneregeneration process has not yet been well-defined. Therefore,in this study, the effect of SS was investigated in the proliferationand differentiation of MC3T3-E1 osteoblastic cell line. Thetreatment of SS did not significantly affect the cell proliferationin MC3T3-E1 cells. SS significantly accelerated themineralization and significantly increased the expression ofalkaline phosphatase (ALP) and osteocalcin (OC) mRNAs,compared to the control, in the differentiation of MC3T3-E1cells. SS significantly accelerated the decrease of osteonectin(ON) mRNA expression as compared with the control in atime-dependent manner in the differentiation of MC3T3-E1cells. These results suggest that the SS facilitate the osteoblastdifferentiation and mineralization in MC3T3-E1 osteoblastic cells. Therefore, there may be potential properties fordevelopment and clinical application of bone regenerationmaterials.
참고문헌 (32)
- Berg C, "Teriparatide", Nat Rev Drug Discov 2, 160, pp.257~258 (2003)
- Ducy P, "The osteoblast : a sophisticated fibroblast under central surveillance", Science, 289, pp.1501~1504 (2000)
- Suda T, "Modulation of osteoclast differentiation", Endocr Rev, 13, pp.66~80 (1992)
- Duda RJ, "Concurnet assays of circulating bone-gla protein and alkaline phosphate : Effect of sex, age, and metabolic bone disease", J Clin Enfocr Meta, 66, pp.951~957 (1998)
- Mohan S, "Bone growth factors", Clin Orthop Relat Res, 263, pp.30~48 (1991)
- Harada S, "Control of osteoblast function and regulation of bone mass", Nature, 423, pp.349~355 (2003)
- Ae-Son Om, "Genistein May Prevent Cadmium-Induced Bone Loss in Ovariectomized Rats", Journal of Medicinal Food, 6(4), pp.337~343 (2003), 한국식품영양과학회
- Bowles SA, "Serum osteocalcin, total and bone-specific alkaline phosphatase following isolated tibial shaft fracture", Ann Clin Biochem, 33, pp.196~200 (1996)
- Anderson JJ, "Phytoestrogens and bone", Baillieres Clin Endocrinol Metab, 12, pp.543~557 (1998)
- Lee HK, "Effect of black bean and samryungbakchulsan on ovariectomy-induced postmenopausal osteoporotic rats", Ph.D., pp.1998
- Setchell KD, "Dietary isoflavones : biological effects and relevance to human health", J Nutr, 129, pp.758~767 (1999)
- Ho JY, "Cell proliferation effcets of Eucommiae and Acanthopanacs methanol extraxts on the osteoblastlike cell line, MG-63 and Saos-2", Ph.D., pp.2001
- 윤선, "가시오가피 추출물의 항산화활성 및 MG-63 조골세포 증식과 alkaline phosphatase 활성에 미치는 효과", 한국식품과학회지, 39(6), pp.694~700 (2007), 한국식품과학회
- 엄소정, "약콩 (Rhynchosia volubilis: 鼠目太) 및 대두 처리에 의한 MG-63 조골세포 증식 증가에서 ERα의 역할에 대한 연구", Journal of Nutrition and Health, 38(7), pp.512~520 (2005), 한국영양학회
- 박종희, "“접골목”의 생약학적 연구", 생약학회지, 37(2), pp.120~123 (2006), 한국생약학회
- Shi XC, "Chinese-English Terminology of Traditional Chinese Medicine", pp.71~78 (1983), Hunan Science Publishing Co
- 이동건, "接骨木추출물 및 약침액에 의한 MIF 활성 조절능이 생쥐의 제2형 Collagen 유발 관절염에 미치는 영향", 대한침구의학회지, 29(1), pp.103~113 (2012), 대한침구의학회
- Nakano Y, "ATP-mediated mineralization of MC3T3-E1 osteoblast cultures", Bone, 41, pp.549~561 (2007)
- Al-Jallad HF, "Transglutaminase activity regulates osteoblast differentiation and matrix mineralization in MC3T3-E1 osteoblast cultures", Matrix Biol, 25, pp.135~148 (2006)
- 국중기, "Antimicrobial Activity of Berberine against Oral Bacteria Related to Endodontic Infections", International Journal of Oral Biology, 38(4), pp.141~147 (2013), 대한구강생물학회[DOI]
- Verhaar HJ, "A comparison of the action of progestins and estrogen on the growth and differentiation of normal adult human osteoblast-like cells in vitro", Bone, 15, pp.307~311 (1994)
- Kassem M, "Growth hormon stimulates proliferation of normal human bone marrow stromal osteoblast precursor cells in vitro", Growth Regul, 4, pp.131~135 (1994)
- Choi EM, "Soybean ethanol extract increases the function of osteo-blastic MC3T3-E1 cells", Phytochemistry, 56, pp.733~739 (2001)
- Moseley JM, "Principles of Bone Biology", pp.363~376 (1996), Academic Press
- Broskey AL, "Non-collagen matrix proteins and their role in mineralization", Bone Miner, 6, pp.111~123 (1992)
- Bellow CG, "Initiation and progression of mineralization of bone nodules formed in vitro : The role of alkaline phosphate and organic phosphate", Bone Miner, 14, pp.27~40 (1991)
- Gilmour DT, "Mice deficient for the secreted glycoprotein SPARC", osteonectin, EMBO J, pp.1998, 17[DOI]
- Brekken RA, "Enhanced growth of tumors in SPARC null mice is associated with changes in the ECM", J Clin Invest, 111, pp.487~495 (2003)
- Kim YW, "Expression of osteopontin and osteonectin in breast cancer", J Korean Med Sci, 13, pp.652~657 (1998)
- Safadi FF, "Expression of connective tissue growth factor in bone : its role in osteoblast proliferation and differentiation in vitro and bone formation in vivo", J Cell Physiol, 196, pp.51~60 (2003)
- Hauschka PV, "Osteocalcin and matrix Gla protein : vitamin K-dependent proteins in bone", Physiol Rev, 69, pp.990~1047 (1989)
- 문연희, "Effects of CoCl2 on Osteogenic Differentiation of Human Mesenchymal Stem Cells", International Journal of Oral Biology, 38(3), pp.111~119 (2013), 대한구강생물학회