홍화씨 추출물이 MC3T3-E1 조골세포의 증식과 분화에 미치는 영향
Influence of Carthamus tinctorius seed extract on proliferation and differentiation of MC3T3-E1 osteoblast cells
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Purpose: The main aim of this study was to determine the effect of Carthamus tinctorius seed extract in the proliferation and differentiation of MC3T3-E1 osteoblastic cell line. Materials and Methods: To determine the characteristic alteration of MC3T3-E1 cells by C. tinctorius, it was examined by cell culture, MTT assay, Von Kossa staining, reverse transcriptase-polymerase chain reaction and quantitative real-time polymerase chain reaction analysis in MC3T3-E1 cells. Results: The treatment of C. tinctorius did not significantly affect the cell proliferation in MC3T3-E1 cells. C. tinctorius significantly accelerated the mineralization as compared with the control culture in the differentiation of MC3T3-E1 cells. C. tinctorius significantly increased the expression of alkaline phosphatase mRNA as compared with the control in the differentiation of MC3T3-E1 cells. C. tinctorius significantly accelerated the decrease of osteonectin mRNA expression as compared with the control in a time-dependent manner in the differentiation of MC3T3-E1 cells. Carthamus tinctorius significantly increased the expression of osteocalcin mRNA as compared with the control in the differentiation of MC3T3-E1 cells. Conclusion: These results suggest that the C. tinctorius facilitate the osteoblast differentiation and mineralization in the differentiation of MC3T3-E1 osteoblastic cells, and therefore, that it may have potential properties for development and its clinical application of bone regeneration materials.
Abstract
Purpose: The main aim of this study was to determine the effect of Carthamus tinctorius seed extract in the proliferation and differentiation of MC3T3-E1 osteoblastic cell line. Materials and Methods: To determine the characteristic alteration of MC3T3-E1 cells by C. tinctorius, it was examined by cell culture, MTT assay, Von Kossa staining, reverse transcriptase-polymerase chain reaction and quantitative real-time polymerase chain reaction analysis in MC3T3-E1 cells. Results: The treatment of C. tinctorius did not significantly affect the cell proliferation in MC3T3-E1 cells. C. tinctorius significantly accelerated the mineralization as compared with the control culture in the differentiation of MC3T3-E1 cells. C. tinctorius significantly increased the expression of alkaline phosphatase mRNA as compared with the control in the differentiation of MC3T3-E1 cells. C. tinctorius significantly accelerated the decrease of osteonectin mRNA expression as compared with the control in a time-dependent manner in the differentiation of MC3T3-E1 cells. Carthamus tinctorius significantly increased the expression of osteocalcin mRNA as compared with the control in the differentiation of MC3T3-E1 cells. Conclusion: These results suggest that the C. tinctorius facilitate the osteoblast differentiation and mineralization in the differentiation of MC3T3-E1 osteoblastic cells, and therefore, that it may have potential properties for development and its clinical application of bone regeneration materials.
참고문헌 (31)
- Berg C, "Teriparatide", Nat Rev Drug Discov, 2, pp.257~258 (2003)
- Ducy P, "The osteoblast: a sophisticated fibroblast under central surveillance", Science, 289, pp.1501~1504 (2000)
- Suda T, "Modulation of osteoclast differentiation", Endocr Rev, 13, pp.66~80 (1992)
- Duda RJ Jr, "Concurrent assays of circulating bone Gla-protein and bone alkaline phosphatase: effects of sex, age, and metabolic bone disease", J Clin Endocrinol Metab, 66, pp.951~957 (1988)
- Mohan S, "Bone growth factors", Clin Orthop Relat Res, 263, pp.30~48 (1991)
- Harada S, "Control of osteoblast function and regulation of bone mass", Nature, 423, pp.349~355 (2003)
- Ae-Son Om, "Genistein May Prevent Cadmium-Induced Bone Loss in Ovariectomized Rats", Journal of Medicinal Food, 6(4), pp.337~343 (2003), 한국식품영양과학회
- Bowles SA, "Serum osteocalcin, total and bone-specific alkaline phosphatase following isolated tibial shaft fracture", Ann Clin Biochem, 33, pp.196~200 (1996)
- Anderson JJ, "Phytoestrogens and bone", Baillieres Clin Endocrinol Metab, 12, pp.543~557 (1998)
- Tsai JN, "Teriparatide and denosumab, alone or combined, in women with post-menopausal osteoporosis: the DATA study randomised trial", Lancet, 382, pp.50~56 (2013)
- Setchell KD, "Dietary isoflavones: biological effects and relevance to human health", J Nutr, 129, pp.758S~767S (1999)
- Catalano A, "Zoledronic acid acutely increases sclerostin serum levels in women with postmenopausal osteoporosis", J Clin Endocrinol Metab, 98, pp.1911~1915 (2013)
- 윤선, "가시오가피 추출물의 항산화활성 및 MG-63 조골세포 증식과 alkaline phosphatase 활성에 미치는 효과", 한국식품과학회지, 39(6), pp.694~700 (2007), 한국식품과학회
- 엄소정, "약콩 (Rhynchosia volubilis: 鼠目太) 및 대두 처리에 의한 MG-63 조골세포 증식 증가에서 ERα의 역할에 대한 연구", Journal of Nutrition and Health, 38(7), pp.512~520 (2005), 한국영양학회
- Shin SY, "The biologic effects of magnoliae cortex extract and safflower seed (Carthamus tinctorius Linne ) extract mixture on PDL cells and osteoblasts", J Korean Acad Periodontol, 28, pp.545~559 (1998)
- Shuai H, "Chinese-English terminology of traditional Chinese medicine", pp.71~78 (1983), Hunan Science & Technology Press
- Koyama N, "Serotonin derivatives, major safflower (Carthamus tinctorius L.) seed antioxidants, inhibit low-density lipoprotein (LDL) oxidation and atherosclerosis in apolipoprotein E-deficient mice", J Agric Food Chem, 54, pp.4970~4976 (2006)
- Nakano Y, "ATP-mediated mineralization of MC3T3-E1 osteoblast cultures", Bone, 41, pp.549~561 (2007)
- Al-Jallad HF, "Transglutaminase activity regulates osteoblast differentiation and matrix mineralization in MC3T3-E1 osteoblast cultures. Matrix Biol 25:135-148, 2006", Matrix Biol, 25, pp.135~148 (2006)
- 김서윤, "Anticancer effects of quercetin on KB human oral cancer cells", 구강생물학연구, 36(2), pp.113~122 (2012), 구강생물학연구소
- Verhaar HJ, "A comparison of the action of progestins and estrogen on the growth and differentiation of normal adult human osteoblast-like cells in vitro", Bone, 15, pp.307~311 (1994)
- Kassem M, "Growth hormone stimulates proliferation of normal human bone marrow stromal osteoblast precursor cells in vitro", Growth Regul, 4, pp.131~135 (1994)
- Choi EM, "Soybean ethanol extract increases the function of osteoblastic MC3T3-E1 cells", Phytochemistry, 56, pp.733~739 (2001)
- Yang JH, "The effect of bone morphogenic protein-2-coated tri-calcium phosphate", hydroxyapatite on new bone formation in a rat model of femoral distraction osteogenesis, 2012, pp.14, 315~326
- Boskey AL, "Noncollagenous matrix proteins and their role in mineralization", Bone Miner, 6, pp.111~123 (1989)
- Bellows CG, "Initiation and progression of mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate", Bone Miner, 14, pp.27~40 (1991)
- Gilmour DT, "Mice deficient for the secreted glycoprotein SPARC", osteonectin, EMBO J, pp.1998, 17[DOI]
- Brekken RA, "Enhanced growth of tumors in SPARC null mice is associated with changes in the ECM", J Clin Invest, 111, pp.487~495 (2003)
- Kim YW, "Expression of osteopontin and osteonectin in breast cancer", J Korean Med Sci, 13, pp.652~657 (1998)
- Safadi FF, "Expression of connective tissue growth factor in bone: its role in osteoblast proliferation and differentiation in vitro and bone formation in vivo", J Cell Physiol, 196, pp.51~62 (2003)
- Hauschka PV, "Osteocalcin and matrix Gla protein: vitamin K-dependent proteins in bone", Physiol Rev, 69, pp.990~1047 (1989)