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Effects of Scytosiphon lomentaria on osteoblastic proliferation and differentiation of MC3T3-E1 cells

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BACKGROUND/OBJECTIVES: Bone formation and bone resorption continuously occur in bone tissue to prevent the accumulation of old bone, this being called bone remodeling. Osteoblasts especially play a crucial role in bone formation through the differentiation and proliferation. Therefore, in this study, we investigated the effects of Scytosiphon lomentaria extract (SLE) on osteoblastic proliferation and differentiation in MC3T3-E1 cells. MATERIALS/METHODS: A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining and protein expression analysis of osteoblastic genes were carried out to assess the osteoblastic proliferation and differentiation. RESULTS: The results indicated that treatment of SLE promoted the proliferation of MC3T3-E1 cells and improved ALP activity. And, SLE treatment significantly promoted mineralized nodule formation compared with control. In addition, cells treated with SLE significantly upregulated protein expression of ALP, type 1 collagen, bone morphogenetic protein 2, runt-related transcription factor 2, osterix, and osteoprotegerin. CONCLUSIONS: The results demonstrate that SLE promote differentiation inducement and proliferation of osteoblasts and, therefore may help to elucidate the transcriptional mechanism of bone formation and possibly lead to the development of bone-forming drugs.

Abstract

BACKGROUND/OBJECTIVES: Bone formation and bone resorption continuously occur in bone tissue to prevent the accumulation of old bone, this being called bone remodeling. Osteoblasts especially play a crucial role in bone formation through the differentiation and proliferation. Therefore, in this study, we investigated the effects of Scytosiphon lomentaria extract (SLE) on osteoblastic proliferation and differentiation in MC3T3-E1 cells. MATERIALS/METHODS: A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining and protein expression analysis of osteoblastic genes were carried out to assess the osteoblastic proliferation and differentiation. RESULTS: The results indicated that treatment of SLE promoted the proliferation of MC3T3-E1 cells and improved ALP activity. And, SLE treatment significantly promoted mineralized nodule formation compared with control. In addition, cells treated with SLE significantly upregulated protein expression of ALP, type 1 collagen, bone morphogenetic protein 2, runt-related transcription factor 2, osterix, and osteoprotegerin. CONCLUSIONS: The results demonstrate that SLE promote differentiation inducement and proliferation of osteoblasts and, therefore may help to elucidate the transcriptional mechanism of bone formation and possibly lead to the development of bone-forming drugs.

참고문헌 (35)

  1. Michaëlsson K, "Genetic liability to fractures in the elderly", Arch Intern Med, 165, pp.1825~1830 (2005)
  2. Slemenda CW, "Genetic determinants of bone mass in adult women: a reevaluation of the twin model and the potential importance of gene interaction on heritability estimates", J Bone Miner Res, 6, pp.561~567 (1991)
  3. Riggs BL, "Sex steroids and the construction and conservation of the adult skeleton", Endocr Rev, 23, pp.279~302 (2002)
  4. Isomura H, "Bone metabolism and oxidative stress in postmenopausal rats with iron overload", Toxicology, 197, pp.93~100 (2004)
  5. Yonezawa T, "Harmine, a β-carboline alkaloid, inhibits osteoclast differentiation and bone resorption in vitro and in vivo", Eur J Pharmacol, 650, pp.511~518 (2011)
  6. Lane NE, "A review of anabolic therapies for osteoporosis", Arthritis Res Ther, 5, pp.214~222 (2003)
  7. Yoon HJ, "Dichloromethane extracts of Sophora japonica L. stimulate osteoblast differentiation in mesenchymal stem cells", Nutr Res, 33, pp.1053~1062 (2013)
  8. Zhang R, "Wnt", β-catenin signaling activates bone morphogenetic protein 2 expression in osteoblasts, 2013, pp.52, 145~156
  9. Demirel Z, "Antimicrobial and antioxidant activity of brown algae from the Aegean sea", J Serb Chem Soc, 74, pp.619~628 (2009)
  10. Nakamura T, "High tocopherol content in a brown alga Ishige okamurae", Fish Sci, 60, pp.793~794 (1994)
  11. Hill PA, "Multiple extracellular signals promote osteoblast survival and apoptosis", Endocrinology, 138, pp.3849~3858 (1997)
  12. Tietz NW, "A reference method for measurement of alkaline phosphatase activity in human serum", Clin Chem, 29, pp.751~761 (1983)
  13. Whyte MP., "Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization", Endocr Rev, 15, pp.439~461 (1994)
  14. Niu YB, "The beneficial effect of Radix Dipsaci total saponins on bone metabolism in vitro and in vivo and the possible mechanisms of action", Osteoporos Int, 23, pp.2649~2660 (2012)
  15. Musiał K, "Osteopontin (OPN), PDGF-BB (platelet-derived growth factor) and BMP-7 (bone morphogenetic protein) as markers of atherogenesis in children with chronic kidney disease (CKD) treated conservatively--preliminary results", Pol Merkuriusz Lek, 24(Suppl 4), pp.25~27 (2008)
  16. W W., "Glial line-derived neurotrophic factor (GDNF): biological activities", Folia Morphol (Warsz), 58, pp.155~159 (1999)
  17. Li F, "Echinacoside promotes bone regeneration by increasing OPG", RANKL ratio in MC3T3-E1 cells, 2012, pp.83, 1443~1450
  18. Lee HS, "Stimulation of osteoblastic differentiation and mineralization in MC3T3-E1 cells by yeast hydrolysate", Phytother Res, 25, pp.716~723 (2011)
  19. Christiansen C., "Consensus development conference: diagnosis, prophylaxis, and treatment of osteoporosis", Am J Med, 94, pp.646~650 (1993)
  20. Rodan GA, "Therapeutic approaches to bone diseases", Science, 289, pp.1508~1514 (2000)
  21. Ducy P, "The osteoblast: a sophisticated fibroblast under central surveillance", Science, 289, pp.1501~1504 (2000)
  22. Hsieh TP, "Icariin isolated from Epimedium pubescens regulates osteoblasts anabolism through BMP-2, SMAD4, and Cbfa1 expression", Phytomedicine, 17, pp.414~423 (2010)
  23. Chandini SK, "In vitro antioxidant activities of three selected brown seaweeds of India", Food Chem, 107, pp.707~713 (2008)
  24. Bai XC, "Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB", Biochem Biophys Res Commun, 314, pp.197~207 (2004)
  25. Neve A, "Osteoblast physiology in normal and pathological conditions", Cell Tissue Res, 343, pp.289~302 (2011)
  26. Bellows CG, "Initiation and progression of mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate", Bone Miner, 14, pp.27~40 (1991)
  27. Horiguchi Y, "Effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of osteoblastic clone MC3T3-E1 cells", Infect Immun, 59, pp.1112~1116 (1991)
  28. Kühn MC, "Adipocyte-secreted factors increase osteoblast proliferation and the OPG", RANKL ratio to influence osteoclast formation, 2012, pp.349, 180~188
  29. Simonet WS, "Osteoprotegerin: a novel secreted protein involved in the regulation of bone density", Cell, 89, pp.309~319 (1997)
  30. Park KH, "Melatonin promotes osteoblastic differentiation through the BMP", ERK, J Pineal Res, pp.2011, 51[DOI]
  31. Huang W, "Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells", Exp Cell Res, 274, pp.226~234 (2002)
  32. Phimphilai M, "BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotype", J Bone Miner Res, 21, pp.637~646 (2006)
  33. Z.P. Liu, "Effects of trans-Resveratrol from Polygonum cuspidatum on Bone Loss Using the Ovariectomized Rat Model", Journal of Medicinal Food, 8(1), pp.14~19 (2005), 한국식품영양과학회
  34. Rassi CM, "Down-regulation of osteoclast differentiation by daidzein via caspase 3", J Bone Miner Res, 17, pp.630~638 (2002)
  35. O'Gorman DM, "The marinederived, multi-mineral formula, Aquamin, enhances mineralisation of osteoblast cells in vitro", Phytother Res, 26, pp.375~380 (2012)

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