인체 조골세포 MG-63 세포증식에 미치는 생약초 추출물의 효과
The Effect of Medicinal Plants Extracts on the Cell Growth in the MG-63 Human Osteoblast Cell
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In the present study we investigated the proliferative effect of human osteoblast MG-63 cell treated with 8 spp. medicinal plant extracts to screen the active compounds . Plant extracts were prepared by ethanol extraction method from Carthamus tinctorius seeds, Rhynchosia volubilis seeds, Camellia japonica leaves, Acanthopanax senticosus root and wood, Eucommia ulmoides bark, Chaenomeles sinensis flesh, Sambucus sieboldiana stem, Achyranthes japonica whole plant. A osteoblast proliferation test was conducted by MTT assay on 24 wellplate. Among 8 extracts, R. volubilis seeds and C. japonica leaves showed relatively strong stimulatory activity on osteoblast proliferation at the concentration of 12.5-200 /mL. Also Chaenomeles sinensis flesh, Sambucus sieboldiana stem, Achyranthes japonica whole plant showed cell viability of above 80 % at the concentration of 25 /mL. These results suggested that 3 extracts may have beneficial effects on alveolar bone health through the proliferation of osteoblast cells.
Abstract
In the present study we investigated the proliferative effect of human osteoblast MG-63 cell treated with 8 spp. medicinal plant extracts to screen the active compounds . Plant extracts were prepared by ethanol extraction method from Carthamus tinctorius seeds, Rhynchosia volubilis seeds, Camellia japonica leaves, Acanthopanax senticosus root and wood, Eucommia ulmoides bark, Chaenomeles sinensis flesh, Sambucus sieboldiana stem, Achyranthes japonica whole plant. A osteoblast proliferation test was conducted by MTT assay on 24 wellplate. Among 8 extracts, R. volubilis seeds and C. japonica leaves showed relatively strong stimulatory activity on osteoblast proliferation at the concentration of 12.5-200 /mL. Also Chaenomeles sinensis flesh, Sambucus sieboldiana stem, Achyranthes japonica whole plant showed cell viability of above 80 % at the concentration of 25 /mL. These results suggested that 3 extracts may have beneficial effects on alveolar bone health through the proliferation of osteoblast cells.
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