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강원도 삼마-태정 산성광산배수의 토착박테리아와 지구화학

The Indigenous Bacteria and the Geochemistry for Acid Mine Drainage in the Abandoned Sama-Taejeong Coal Mine, Gangwon-do

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초록

도계 삼마-태정 폐탄광에 형성된 산성광산배수의 지구화학적 특성과 광산배수 중에 서식하는 토착박테리아의 존재를 조사하고자하였다. 크기가 0.3×1.3 ㎛에서 0.4×3.4 ㎛ 범위인 막대 모양의 박테리아들이 산성광산배수에서 관찰되었으며 협막을 형성하는 박테리아는 황갈색 철수산화물에서 관찰되었다. 이들 막대 모양의 토착박테리아들이 원소 황과 Fe2+ 이온이 포함된 성장-배양액에서 성장되었다. 막대 모양의 박테리아들이 성장함에 따라 성장-배양액의 pH는 3.72에서 2.08로 감소하였고, Eh는 증가하였다. 성장-배양액과 계대배양에서 이들 막대 모양의 토착박테리아들이 배양됨에 따라 이들 박테리아들은 Fe2+ 이온과 원소 황을 활용하는 박테리아들임이 밝혀졌다. 성장-배양액과 계대배양에서 감소하는 pH 값과 증가하는 Eh 값은 막대 모양의 토착박테리아들이 Fe2+ 이온과 원소 황을 활용하고 있음이 확인되었다. 황갈색 철수산화물에 대한 제타전위를 측정한 결과 등전위가 4.1에서 7.1로 나타났다.

Abstract

This study was to investigate the geochemistry and the indigenous acidophilic bacteria in the acid mine drainage from the abandoned Sama-Taejeong coal mine, Gangwon-do. The size of the rod-shaped bacteria observed in the acid mine drainage ranged from 0.3×1.3 ㎛ to 0.4×3.4 ㎛, and sheathed-filament bacteria were observed in the yellow-colored iron hydroxide. These rod-shaped indigenous bacteria were grown in a growth- medium containing elemental sulfur and Fe2+ ion. As the rod-shaped bacteria were growing, the pH value was decreased from 3.72 to 2.08, whereas the Eh values were increased in the growth-medium. In decreasing the pH value and increasing the Eh value in the growth-medium and the subculture, it was identified that the rod-shaped indigenous bacteria were utilized the Fe2+ and elemental sulfur. The zero point of charge of the yellow-colored iron hydroxide were determined to range from 4.1 to 7.1 using zeta potential.

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