토착호산성박테리아의 황철석 표면 부착과 용출 특성
The Characteristics of Attachment on Pyrite Surface and Bioleaching by Indigenous Acidophilic Bacteria
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본 연구는 산성광산배수에 서식하고 있는 토착호산성박테리아를 이용하여 황철석의 용출특성을 조사하고자하였다. 토착호산성 박테리아들이 황철석을 산화시킬 때 세포외중합체물질이 황철석 표면에 그리고 황철석 입자를 둘러쌓고 있는 것이 관찰되었다. 세포외중합체물질은 토착박테리아들에 의하여 형성된 것으로 보여 지며 생물막 기능이 있는 것으로 해석된다, 세포외중합체를 EDS분석한 결과 C, O, P, S, K 및 Fe가 검출되었다. 크기가 1.19～1.42 ㎛인 막대모양의 토착호산성 박테리아들이 황철석 표면의 파단면에 집중적으로 부착하였다. 이는 에너지를 쉽게 얻을 수 있는 장소를 박테리아들이 선택하는 것으로 보인다. 황철석-용출 배양액의 pH와 Eh 변화에서 비교시료에서 보다 박테리아 시료에서 pH 값이 낮게 그리고 Eh는 높게 나타났다. 이는 토착호산성박테리아가 황철석을 용출하였기 때문이다. 황철석-용출 배양액의 광액농도가 증가하면 용출되는 Zn 함량이 감소하였다.
Abstract
This study is to investigate the bioleaching of pyrite using indigenous acidophilic bacteria from the acid mine drainage. When the indigenous bacteria oxidized the pyrite, the extracellular polymeric substance (EPS) was formed and coated surface of pyrite grains. The formation of the EPS was probably secreted by the indigenous bacteria, and the EPS contributed to the bio-film function. In the EDS analysis, the elements C, O, P, S, K and Fe were detected in the EPS. Numerous indigenous bacteria, of which size range between 1.19 to 1.42 ㎛ were attached along the cracks on the pyrite surface. These bacteria are able to actively select the sites from which is most ease to gain energy through oxidation. On the pyrite-leaching medium, the pH values were higher in the bacterial sample than the control sample, whereas the Eh values were lower in the bacteria sample than in the control sample, because the indigenous acidophilic bacteria were capable of bioleaching the pyrite. The Zn content in the pyrite-leaching medium decreased with an increase in the pulp density.
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