Acidophilic Bacterial Communities of Soil and Enrichment Cultures from Two Abandoned Mine Sites of the Korean Peninsula
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Bacterial diversity based on the denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA gene sequences was determined for soil samples from two abandoned mine sites and the corresponding enrichment cultures using soil sample as key inoculum. Sequencing analysis of DGGE bands obtained from both the soil samples matched mostly with sequences of uncultured and newly described organisms, or organisms recently associated with the acid mine drainage environment. However, the enrichment of soil samples in ferrous sulfate and elemental sulfur media yielded sequences that were consistent with well-known iron- and sulfur-oxidizing acidophilic bacteria. Analysis of enrichment cultures of soil samples from Dalsung mine revealed abundant γ-Proteobacteria, whereas that of Gubong mine sample displayed acidophilic groups of γ-Proteobacteria, α-Proteobacteria, Actinobacteria and Firmicutes. Chemical elemental analysis of the mine samples indicated that the Dalsung site contained more iron and sulfate along with other toxic components as compared with those of the Gubong site. Biogeochemistry was believed to be the primary control on the acidophilic bacterial group in the enrichment samples.
Abstract
Bacterial diversity based on the denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA gene sequences was determined for soil samples from two abandoned mine sites and the corresponding enrichment cultures using soil sample as key inoculum. Sequencing analysis of DGGE bands obtained from both the soil samples matched mostly with sequences of uncultured and newly described organisms, or organisms recently associated with the acid mine drainage environment. However, the enrichment of soil samples in ferrous sulfate and elemental sulfur media yielded sequences that were consistent with well-known iron- and sulfur-oxidizing acidophilic bacteria. Analysis of enrichment cultures of soil samples from Dalsung mine revealed abundant γ-Proteobacteria, whereas that of Gubong mine sample displayed acidophilic groups of γ-Proteobacteria, α-Proteobacteria, Actinobacteria and Firmicutes. Chemical elemental analysis of the mine samples indicated that the Dalsung site contained more iron and sulfate along with other toxic components as compared with those of the Gubong site. Biogeochemistry was believed to be the primary control on the acidophilic bacterial group in the enrichment samples.
참고문헌 (26)
- Denef VJ, "AMD biofilms: Using model communities to study microbial evolution and ecological complexity in nature", International Society for Microbial Ecology, 4, pp.599~610 (2010)
- Druschel GK, "TM Gihring and JF Banfield. 2004. Acid mine drainage biogeochemistry at Iron Mountain", California Geochemical Transactions, 5, pp.13~32 (2004)
- Dufresne S, "Sulfobacillus disulfidooxidans sp. nov., a new acidophilic, disulfide- oxidizing, gram-positive, spore-forming bacterium", International Journal of Systematic Bacteriology, 46, pp.1056~1064 (1996)
- Gonzalez-Toril E, "Microbial ecology of Rio Tinto, a natural extreme acidic environment of biohydrometallurgical interest", Hydromet, 104, pp.329~333 (2010)
- Gurung A, "The role of Acidithiobacillus ferrooxidans in alleviating the inhibitory effect of thiosulfate on the growth of acidophilic Acidiphilum species isolated from acid mine drainage samples from Garubathan, India", Canadian Journal of Microbiology, 55, pp.1040~1048 (2009)
- Jaffery GH, "Text book of quantitative chemical analysis (5th Edition)", pp.490~493 (1989), Longman Scientific and Technical
- Joe SJ, "Isolation and characterization of acidophilic heterotrophic iron-oxidizing bacterium from enrichment obtained from mine drainage treatment plant", Journal of Bioscience and Bioengineering, 104, pp.117~123 (2007)
- 정명채, "Heavy metal contamination of soils and waters in and around the Imcheon Au-Ag mine, Korea", APPLIED GEOCHEMISTRY, 16, pp.1369~1375 (2001), PERGAMON-ELSEVIER SCIENCE LTD
- 정명채, "Heavy metal concentrations in soils and factors affecting metal uptake by plants in the vicinity of a Korean Cu-W mine", SENSORS, 8, pp.2413~2423, MOLECULAR DIVERSITY PRESERVATION INT
- Kim JS, "Influence of acid mine drainage on microbial communities in stream and groundwater samples at Guryong Mine, South Korea", ENVIRONMENTAL GEOLOGY, 58, pp.1567~1574 (2009), SPRINGER
- Kulp TR, "Arsenic (III) fuels anoxygenic photosynthesis in hot spring biofilms from Mono Lake, California", Science, 321, pp.967~970 (2008)
- Lee SH, "Production of medium-chain-length polyhydroxyalkanoates by activated sludge enriched under periodic feeding with nonanoic acid", Bioresource Technology, 102, pp.6159~6166 (2011)
- Liang HC, "Minerals and mine drainage", Water Environment Research, 81, pp.1615~1663 (2009)
- Mishra D, "Bioleaching of spent hydro-processing catalyst using acidophilic bacteria and its kinetic aspects", Journal of Hazardous Materials, 152, pp.1082~1091 (2008)
- Debaraj Mishra, "Bioleaching: A Microbial Process of Metal Recovery; A Review", Metals and Materials International, 11(3), pp.249~256 (2005), 대한금속·재료학회
- Mishra D, "Current research trends of microbiological leaching for metal recovery from industrial wastes, In Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology Volume 2", pp.1289~1296 (2010), Formatex Research Center
- Mu-quing Q, "A comparison of bioleaching of chalcopyrite using pure culture or a mixed culture", Minerals Engineering, 18, pp.987~990 (2005)
- Muyzer G, "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S rRNA", Applied and Environmental Microbiology, 59, pp.695~700 (1993)
- Nicomrat D, "Assessment of the microbial community in a constructed wetland that receives acid coal mine drainage", Microbial Ecology, 51, pp.83~89 (2006)
- Rawlings DE, "Heavy metal mining using microbes", Annual Review of Microbiology, 56, pp.65~91 (2002)
- Rawlings DE, "microbiology of biomining: development and optimization of mineral-oxidizing microbial consortia", Microbiology, 153, pp.315~324 (2007)
- Sahl JW, "Subsurface microbial diversity in deep-granitic-fracture water in Colorado", Applied and Environmental Microbiology, 74, pp.143~152 (2008)
- Schippers A, "The biogeochemistry and microbiology of sulfidic mine waste and bioleaching dumps and heaps, and novel Fe (II)-oxidizing bacteria", Hydromet, 104, pp.342~350 (2010)
- Schippers A, "Microbiological pyrite oxidation in a mine tailings heap and its relevance to death of vegetation", Geomicrobiol Journal, 17, pp.151~162 (2000)
- Tamura K, "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0", Molecular Biology and Evolution, 24, pp.1596~1599 (2007)
- Tan G-L, "Culturable and molecular phylogenetic diversity of microorganisms in an open-dumped, extremely acidic Pb", Zn mine tailings, 2008, pp.12, 657~664