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화순 폐석탄 광산배수에 나타나는 미생물 기원의 협막과 나선형 구조물에 축적된 비소 특성

Bioaccumulation of Arsenic on the Sheathed-filament and Twisted-stalk Structures from the Microbial Origin in the Hwasun Abandoned Coal Mine Drainage, Korea

조강희|박천영
한국자원공학회 (The Korean Society of Mineral and Energy Resources Engineers)|2010.06|한국자원공학회지 (Journal of the Korean Society of Mineral and Energy Resources Engineers)|제47권 제3호|pp. 369-382

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참고문헌

키워드

광산배수비소흡착Mine drainage황갈색 철수산화물협막 및 나선형-줄기 구조물Yellow-colored iron-hydroxideSheathed-filament and twisted-stalk structureArsenic adsorption
주제분류자원공학

초록

화순 폐광산에서 생성되는 광산배수와 황갈색 철수산화물에 대한 지구화학적 특징을 조사하였다. 항도로부터 배출되는 광산배수의 pH는 7.19에서 8.03으로 변화하였고, 광산배수에 용해되어 있는 Fe 이온은 0.048mg/l에서 1.6 mg/l로 나타났다. 그리고 광산배수로부터 형성된 황갈색 철수산화물에는 Fe2O3 함량이 22.87 wt.%에서 55.07 wt.%로 나타났다. 광산배수에는 As 이온 함량이 76.94 ㎍/l에서 167.9 ㎍/l로 용해되어 있고 황갈색철수산화물에 As 함량이 8400～21300 mg/kg으로 매우 높게 나타났다. 광산배수 중에 용해되어 있는 As 이온이황갈색 철수산화물에 흡착 및 공침전으로 흡착된 것으로 사료된다. 이 비정질의 철수산화물은 광산배수에 용해되어 있는 As 이온은 물론 중금속이온도 제거할 수 있는 흡착제로 사용될 수 있을 것으로 사료된다. 비정질철수산화물에서 협막 구조물과 나선형-구조물이 관찰되었다. 이들 구조는 철수산화물을 생성하는 미생물체로사료되며, 협막 구조물과 나선형구조물에 부착된 철수산화물에서 Fe와 As가 EDS분석에서 검출되었다. 협막및 나선형 구조물이 미생물 기원임을 확인하기 위하여 광산배수와 철수산화물의 혼합물을 펩톤과 FeSO4･7H2O 가 포함된 배양용액에서 배양하였다. 협막 구조물과 나선형의 구조물이 배양용액에서 뚜렷하게 관찰되었다.

Abstract

The objective of this study was to investigate the geochemistry of mine drainage and the yellow-colored iron hydroxide formed in the abandoned Hwasun coal mine. The pH value of the acid mine drainage from the adit of the mine varied from 7.19 to 8.03. The mine drainage contained dissolved Fe ion in the range of 0.048mg/l to 1.6 mg/l, which formed yellow-colored iron-hydroxide. The iron-hydroxide contained Fe2O3 in the range of 22.87 wt.% to 55.07 wt.%. The As ion in the mine drainage was dissolved with As concentrations in the range of 76.94 ㎍/l to 167.9 ㎍/l, and the content of As in the yellow-colored iron-hydroxide was in the range of 8400 mg/kg to 21300 mg/kg. It was thought that the dissolved As ion in the mine drainage may be adsorbed by the yellow-colored iron-hydroxide due to adsorption and co-precipitation. This amorphous iron-hydroxide would be used as an absorbent to remove the As ion as well as heavy metal ions in the mine drainage. Sheathed-filament and twist-stalk structures were observed in the amorphous iron-hydroxide. It is suggested that this is a microorganism that produces the iron-hydroxide. The EDS analysis detected Fe and As ions on the iron-hydroxide which were attached to the sheathed-filament and the twisted-stalk were detected by EDS analysis. To identify the sheathed-filament and twisted-stalk as a micro-organism, the mixture of mine water and iron-hydroxide in the mine drainage were cultured in a medium which contained peptone with FeSO4･7H2O at 32℃ for 7 days. The sheathed-filament and twisted-stalk structures were observed clearly in the medium.

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