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Effects of treadmill exercise on hippocampal cell proliferation and memory in streptozotocin rat model for sporadic Alzheimer's disease

김창주|윤진환|정일규|이희혁
한국운동생리학회|2007.02|운동과학 (Exercise Science)|제16권 제1호|pp. 11-22|DOI 10.15857/ksep.2007.16.1.11

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키워드

기억력해마트레드밀 운동신경세포생성알쯔하이머병Seoul Olympic Sports Promotion Foundation. (KISS-06-A06006).

초록

정일규, 윤진환, 이희혁, 김창주. 트레드밀 운동이 알쯔하이머 흰쥐 모델에서 해마의 신경세포 생성과 기억력에 미치는 영향. 운동과학, 제16권 제1호. 11-22, 2007. 본 연구의 목적은 알쯔하이머 흰쥐 모델에서 트레드밀 운동이 해마의 신경세포생성과 인지기능에 미치는 효과를 검증하는데 있다. 9주령의 Sprague- Dawley계 흰쥐 수컷 36마리를 3집단으로 나누어 정상 통제군, 알쯔하이머군, 그리고 알쯔하이머 운동군으로 구분하였다. 알쯔하이머형 치매는 양쪽 대뇌 뇌실에 streptozotocin (1.5mg/kg)을 주입해 유도했다. 치매 유발 후, 운동군의 동물들은 2주간 매일 30분씩 실험동물용 트레드밀에서 달리도록 하였다. 기억력 행동검사는 passive avoidance와 elevated plus maze를 통해 각각 검사되었다. 모든 실험 처치 24시간 후 실험쥐들을 마취한 후 심장을 통한 관류를 실시하여 뇌를 고정한 후 꺼내어 40㎛로 절개하여 조직을 준비하였고, 신경세포 생성을 측정하기 위하여 5-bromo-2'-deoxyuridine (BrdU) 면역조직화학법을 실시하였다. 본 연구결과 알쯔하이머형 치매흰쥐에서 기억력이 정상흰쥐에 비해 유의하게 손상된 것으로 나타났다. 또한 해마의 신경세포생성도 치매흰쥐에서 유의하게 감소된 것으로 나타났다. 하지만 알쯔하이머형 치매흰쥐에서 트레드밀 운동은 기억력 손상 및 해마의 신경세포생성 감소를 통계적으로 유의하게 상쇄시키는 것으로 나타났다. 본 연구결과 STZ로 유발된 알쯔하이머형 치매흰쥐에서 운동이 신경세포생성을 증가시킴으로써 기억력을 향상시킬 수 있음을 보여주었다.

Abstract

Jeong, I.G., Yoon, J.H., Lee, H.H., Kim, C.J. Effects of treadmill exercise on hippocampal cell proliferation and memory in streptozotocin rat model for sporadic Alzheimer's disease. Exercise Science, 16(1): 11-22, 2007. In the present study, we examined the effects of treadmill exercise on memory ability and cell proliferation in the dentate gyrus in rat model for sporadic Alzheimer's disease. For the induction of sporadic Alzeimer's type dementia, adult male rats were injected with intracerebroventricular (ICV) streptozotocin (STZ) bilaterally. Rats in the exercise group were forced to run on a treadmill for 30 min daily for 14 days. The memory function was assessed using a passive avoidance task and elevated plus maze task, respectively. Cell proliferation was assessed by the 5-bromo- 2'-deoxyuridine (BrdU) immunohistochemistry. In the present study, ICV STZ-injected rats showed impaired memory function, and treadmill exercise in ICV STZ-injected rats alleviated the impairment of learning and memory. We also demonstrated that the number of proliferating cells in the dentate gyrus was markedly decreased in ICV STZ-injected rats. However, treadmill exercise significantly enhanced cell proliferation in the dentate gyrus of ICV- STZ-injected rats. The present results suggest that treadmill exercise may be useful in the alleviation of memory dysfunction in ICV STZ-injected rats.

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