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트레드밀 운동이 헌팅턴병 유발 쥐의 뇌신경세포생성과 사멸 및 신경가소성에 미치는 영향

The Effects of Treadmill Exercise on Neurogenesis, Apoptosis and Neuroplasticity in Huntington's Disease Rat Model

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초록

본 연구의 목적은 트레드밀 운동이 헌팅턴 유발 흰쥐의 신경보호에 미치는 영향을 규명하기 위하여 공간 기억력과 관련된 행동검사(Y-maze test)와 뇌신경세포생성(Ki-67) 정도 및 신경세포사멸 관련 단백질(Bid, Bcl-xl)의 발현을 확인함으로서 운동이 뇌의 신경가소성을 향상시킬 수 있는지를 과학적으로 규명하여, 실제 임상에서 헌팅턴 환자들에게 도움을 주는 데 있어 선행연구로서 기초 자료를 제공하는 데 궁극적인 목적이 있다. 6주령 Sprague-Dawley 계열 수컷 흰쥐(200±10 g) 40마리를 무작위 추출법에 의해서 대조 그룹(sham-operation group; n=10), 대조 운동그룹(sham-operation & treadmill exercise group; n=10), 헌팅턴 유발그룹(Huntington’s disease group; n=10), 헌팅턴 유발 후 운동그룹(Huntington’s disease & treadmill exercise group; n=10)으로 분류하였다. 헌팅턴 유발 후 운동그룹에게 트레드밀 운동(2주, 5일/주, 30분/일)을 시킨 결과, 헌팅턴 유발그룹에 비해 공간 기억력과 뇌신경세포생성 및 사멸의 모든 변인에서 통계적으로 유의하게 향상되었음을 확인하였다(p<0.05). 이상의 결과를 토대로, 트레드밀 운동은 헌팅턴 환자의 공간 기억력과 뇌신경세포에 긍정적 효과가 나타남으로써 헌팅턴병의 예방 및 개선에 도움을 줄 수 있을 것으로 생각된다.

Abstract

The aim of the present study was to investigate the effects of treadmill exercise on neurogenesis (Ki-67), apoptosis (Bid, Bcl-xl), and spatial memory (Y-maze test) in the huntington's disease rat model. Forty male rats were divided into sham-operation group (n=10), sham-operation & treadmill exercise group (n=10), Huntington's disease group (n=10), and Huntington's disease & treadmill exercise group (n=10) at the age of 6 weeks. Rats in the treadmill exercise group performed treadmill exercises (5 days/week, 30 min/day) for 2 weeks. The present results found Huntington's disease & treadmill exercise group significantly enhanced hippocampal neurogenesis, apoptosis, and spatial memory compared to the Huntington's disease group (p<0.05). These results indicate that treadmill exercise improves brain functions of rats with Huntington’s disease.

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Effects of treadmill exercise on hippocampal cell proliferation and memory in streptozotocin rat model for sporadic Alzheimer's disease김창주, 윤진환, 이희혁, 정일규한국운동생리학회 · 운동과학2007 · 제16권 제1호뇌 타박 손상 후 해마에서 신경세포 생성 및 신경가소성 조절 단백질 발현에 미치는 운동 기간에 따른 효과백일영, 서태범, 윤진환, 장문녀국민체육진흥공단 한국스포츠과학원 · 체육과학연구2011 · 제22권 제3호트레드밀 운동이 외상성 뇌손상 흰쥐 해마에서 신경세포생성 및 BDNF 발현에 미치는 영향고일규, 김보균, 김성은, 김종오, 김창주, 서진희, 신말순, 윤진환, 이희혁, 정일규한국운동생리학회 · 운동과학2008 · 제17권 제3호트레드밀 운동이 추위 스트레스에 의한 생쥐 해마의 신경세포 사멸에 미치는 영향김기정, 김창주, 백성수, 이신호, 지은상한국운동생리학회 · 운동과학2009 · 제18권 제4호규칙적인 운동이 성인 백서 해마 치상회에서 신경세포의 Dendritic Lengths 및 SynapsinⅠ 수준에 미치는 영향김현태, 이효철국민체육진흥공단 한국스포츠과학원 · 체육과학연구2012 · 제23권 제4호자발 운동 및 부유 환경 조성에 의한 선조체 내 신경세포 생성 유도김은상, 민경훈, 박은숙, 박창일, 배병우, 임상희, 조성래대한재활의학회 · Annals of Rehabilitation Medicine2008 · 제32권 제6호

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