트레드밀 운동이 헌팅턴병 유발 쥐의 뇌신경세포생성과 사멸 및 신경가소성에 미치는 영향
The Effects of Treadmill Exercise on Neurogenesis, Apoptosis and Neuroplasticity in Huntington's Disease Rat Model
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본 연구의 목적은 트레드밀 운동이 헌팅턴 유발 흰쥐의 신경보호에 미치는 영향을 규명하기 위하여 공간 기억력과 관련된 행동검사(Y-maze test)와 뇌신경세포생성(Ki-67) 정도 및 신경세포사멸 관련 단백질(Bid, Bcl-xl)의 발현을 확인함으로서 운동이 뇌의 신경가소성을 향상시킬 수 있는지를 과학적으로 규명하여, 실제 임상에서 헌팅턴 환자들에게 도움을 주는 데 있어 선행연구로서 기초 자료를 제공하는 데 궁극적인 목적이 있다. 6주령 Sprague-Dawley 계열 수컷 흰쥐(200±10 g) 40마리를 무작위 추출법에 의해서 대조 그룹(sham-operation group; n=10), 대조 운동그룹(sham-operation & treadmill exercise group; n=10), 헌팅턴 유발그룹(Huntington’s disease group; n=10), 헌팅턴 유발 후 운동그룹(Huntington’s disease & treadmill exercise group; n=10)으로 분류하였다. 헌팅턴 유발 후 운동그룹에게 트레드밀 운동(2주, 5일/주, 30분/일)을 시킨 결과, 헌팅턴 유발그룹에 비해 공간 기억력과 뇌신경세포생성 및 사멸의 모든 변인에서 통계적으로 유의하게 향상되었음을 확인하였다(p<0.05). 이상의 결과를 토대로, 트레드밀 운동은 헌팅턴 환자의 공간 기억력과 뇌신경세포에 긍정적 효과가 나타남으로써 헌팅턴병의 예방 및 개선에 도움을 줄 수 있을 것으로 생각된다.
Abstract
The aim of the present study was to investigate the effects of treadmill exercise on neurogenesis (Ki-67), apoptosis (Bid, Bcl-xl), and spatial memory (Y-maze test) in the huntington's disease rat model. Forty male rats were divided into sham-operation group (n=10), sham-operation & treadmill exercise group (n=10), Huntington's disease group (n=10), and Huntington's disease & treadmill exercise group (n=10) at the age of 6 weeks. Rats in the treadmill exercise group performed treadmill exercises (5 days/week, 30 min/day) for 2 weeks. The present results found Huntington's disease & treadmill exercise group significantly enhanced hippocampal neurogenesis, apoptosis, and spatial memory compared to the Huntington's disease group (p<0.05). These results indicate that treadmill exercise improves brain functions of rats with Huntington’s disease.
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