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단기간의 수영운동이 흰쥐 골격근 내 젖산, MCT1 발현 및 혈중 케톤체 농도에 미치는 영향

Effects of Short-term Swimming Exercise Training on Lactate Levels, MCT 1 Contents and Circulating Ketone Bodies in Rat Skeletal Muscle

이호성
대한운동학회 (Korean Acadaemy of Kinesiology)|2013.10|아시아 운동학 학술지 (The Asian Journal of Kinesiology)|제15권 제4호|pp. 33-40|DOI 10.15758/jkak.2013.15.4.33

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키워드

젖산Skeletal muscleLactate골격근수영운동MCT1케톤체Swimming exercise trainingMonocarboxylate transporter 1Ketone body

초록

[서론] 이 연구는 단기간의 수영운동이 흰쥐 골격근 내 젖산, MCT1 발현 및 혈중 케톤체 농도에 미치는 영향을 규명하는데 그 목적이 있다. [방법] 수영운동에서 실험동물은 8주령의 Sprague-Dawley (SD)수컷 흰쥐 총 32마리를 대상으로 하였으며, 수영운동은 1회 60분간, 주 7회, 3주간에 걸쳐서 실시하였다. 골격근내의 젖산은 운동직후(IA, n=8)에 측정하였으며, 골격근(가자미근, 장지신근), 심장근 및 간장의 MCT1 단백질양은 운동전(pre, n=8), 7일 후(7D, n=8) 및 21일 후(21D, n=8)에 각각 측정하였다. Glucocorticoid 투여실험에서 실험동물은 8주령의 Sprague-Dawley (SD)수컷 흰쥐 총 14마리를 투여군(administration group; n=7)과 대조군(control group; n=7)으로 분류하였으며, 투여군은 초산 프레드니솔론(prednisolone acetate 2mg/kg)을, 대조군은 생리식염수를 4일간 매일 피하 투여하였으며, 투여 4일 후에 혈액을 채취하여 MCT1 단백질양 및 혈중 케톤체 농도를 측정하였다. [결과] 가자미근 및 장지신근의 젖산농도는 운동전과 비교해서 운동직후에 유의하게 증가하는 것으로 나타났다(p<.005, p<.008 respectively). 가자미근 및 심장근의 MCT1 단백질양은 운동전과 비교해서 운동 7일 후 및 운동 21일 후에 각각 유의하게 증가하는 것으로 나타났다(p<.032, p<.005; p<.037, p<.028 respectively). 또한 간장의 MCT1 단백질양은 운동전과 비교해서 운동 21일 후에 유의하게 증가하는 것으로 나타났다(p<.037). 간장의 MCT1 단백질양 및 혈중 케톤체 농도는 대조군과 비교해서 투여군에 있어서 유의하게 증가하는 것으로 나타났다(p<.027, p<.021 respectively). [결론] 이상의 결과에 의하면, 이 연구에서는 단기간의 수영운동이 세포내에서의 내인성 monocarboxylic acid 및 양성자의 생성이 골격근과 간장의 MCT1을 유도할 가능성이 시사되었다.

Abstract

[INTRODUCTION] The purpose of this study was to examine the effect of short-term swimming exercise training on lactate levels, MCT 1 contents and circulating ketone bodies in rat skeletal muscle. [METHOD] Male Sprague-Dawley rats (N=32) were performed swimming exercise training for 60 minute/day and 7 days/week, for 3 weeks. Lactate in rat skeletal muscle was measured immediately after (IA, n=8), and MCT 1 protein in soleus muscle, extensor digitorum longus muscle and liver were measured before exercise (pre, n=8), after 7 days (7D, n=8) and 21 days (21D, n=8). In Glucocorticoid administration, Male Sprague-Dawley rats (N=14) were assigned to administration group (AG, n=7) and control group (CON, n=7). Administration group was subcutaneous injected prednisolone acetate (2mg/kg) and normal saline in control group, for 4 days, and than MCT 1 protein and blood ketone body were measured after injections. [RESULT] Lactate concentration in soleus muscle and extensor digitorum longus muscle were significantly increased immediately after of swimming exercise training compared with before exercise (p=.005, p=.008 respectively). MCT 1 protein in soleus muscle, extensor digitorum longus muscle were significantly increased after both 7 and 21 days of swimming exercise training compared with before exercise (p=.032, p=.005; p=.037, p=.028 respectively), and MCT 1 protein in liver was significantly increased after 21 days of swimming exercise training (p=.037). In Glucocorticoid administration, MCT 1 protein in liver and blood ketone body concentration were significantly increased in the administration group than in the control group (p=.027, p=.021 respectively). [CONCLUSION] These results suggest that product of intracellular endogenous monocarboxylic and proton induces MCT 1 in the skeletal muscle and liver during short-term swimming exercise training.

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