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L-arginine와 GPLC의 투여가 고교 축구선수의 혈중 산화질소, 에너지기질 및 피로 물질 변화에 미치는 영향

The Effects of L-arginine and GPLC supplementation on plasma nitric oxide, energy substrates and fatigue factors in high school football players

곽이섭|권훈겸|양승훈
한국운동생리학회|2015.08|운동과학 (Exercise Science)|제24권 제3호|pp. 233-242|DOI 10.15857/ksep.2015.24.3.233

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키워드

산화질소L-argininenitric oxide에르고제닉에이드GPLC에너지 기질피로 물질ergogenic aidsenergy substrates and fatigue factor

초록

본 연구의 목적은 엘리트 축구선수에게 있어 ergogenic aids(L-arginine, GPLC) 투여에 따른 산화질소, 에너지 기질 수준의 변화, 혈중 피로물질 발현의 차이를 규명하기 위해 실제 축구경기와 유사한 유 ·무산소성 복합운동 형태의 Yo-Yo intermittent recovery test(Level2)라는 테스트 방법을 이용하여 향후 엘리트 축구선수의 경기력 수행에 있어 ergogenic aids(L-arginine, GPLC) 투여 효과에 대한 자료를 제공하고자 한다. 이러한 본 연구의 목적을 달성하기 위하여 고등학교 엘리트축구 선수 16명을 대상으로 테스트 전 기본검사(신장, 체중, 체지방 등)를 실시하였으며, ergogenic aids(L-arginine, GPLC) 투여 전과 후에 실험에서 각각 3회(운동 전, 운동 직후, 회복기 30분)의 채혈을 실시하여 혈중 산화질소, 피로물질(Lactate, ammonia, phosphorus), 에너지기질(Glucose, FFA)를 각각 측정하여 분석하였다. 유·무산소 복합운동 수행 시에 엘리트 축구선수에게 있어 ergogenic aids의 투여(L-arginine, GPLC)시 NO 농도변화는 두 그룹 모두 투여 전에 비하여 투여 후 각 시기에서 증가한 경향을 보였고, L-arginine 투여 그룹에서의 회복기에서 유의한 차를 나타내었다. 에너지 기질 변화는 인체 혈청 glucose농도는 L-arginine그룹에서 매 시기 마다 통계적으로 유의하게 감소하였으며 GPLC 그룹에서는 운동직후, 회복기에서 유의하게 감소하였다. ergogenic aids(L-arginine, GPLC) 투여에 따른 혈청 유리지방산의 농도는 모든 시기에서 통계적으로 유의한 차이가 나타나지 않았다. 피로물질 중 혈장 phosphorus농도 변화는 L-arginine 그룹에서 매 시기마다 유의하게 감소하였고, GPLC그룹에서는 회복 시에 유의하게 감소한 결과가 나타났다. GPLC그룹에서는 회복 시에 유의한 차로 감소한 결과가 나타났다. Ammonia는 GPLC그룹에 비해 L-arginine그룹에서 투여 전에 비해 투여 후 매 시기 마다 유의한 차이로 ammonia농도가 감소하였고 GPLC 그룹은 운동전, 회복기에서 유의한 차이가 나타났다. Lactate는 L-arginine그룹에서 투여 후 매 시기 유의하게 감소하였고 GPLC그룹에서는 ammonia와 마찬가지로 운동 전과 운동 직후에 유의하게 감소하였다. 이상의 연구결과를 종합해 볼 때 ergogenic aids(L-arginine, GPLC)의 투여로 인해 혈중 산화질소의 농도가 증가하였으며, Lactate, Ammonia, Phosphorus와 같은 피로물질의 농도는 감소되었다. 에너지 기질 측면에서 혈중 FFA(Free Fatty acids)의 농도는 투여 전에 비해 투여 후 운동 중에 더 높은 수치를 보였고 혈중 glucose의 농도는 감소하였다.

Abstract

The purpose of this study was to investigate the effects of L-arginine and GPLC supplementation on plasma nitric oxide, energy substrates and fatigue factor to soccer players. 16 soccer players were recruited from the high school and they were divided into two groups(L-arginine group, n=8; GPLC group, n=8). It also aims to produce data on the effect of ergogenic aids(L-arginine, GPLC) administration in the performance of elite football players using Yo-Yo intermittent recovery test(Level 2) test method, which is aerobic-anaerobic mixed exercise similar to actual football game. For this purpose, basic examination (height, weight and body fat) was performed before the test to 16 high school elite football players. At the experiment of pre-administration and post-administration of ergogenic aids(L-arginine, GPLC), blood was collected 3 times (before exercise, after exercise and 30 minutes at recovery stage) to measure and analyze nitrogen oxide in the blood, fatigue substances (lactate, ammonia and phosphorus) and energy substrate (Glucose and FFA) respectively. During aerobic-anaerobic mixed exercise, when ergogenic aids (L-arginine and GPLC) was administered to the elite football players, NO concentration increased to two groups after administration compared to pre-administration at each stage. There was significant difference at the recovery stage to L-arginine administration group. As for change of energy substrate, human serum glucose concentration decreased significantly in statistics at each stage to L-arginine group, and decreased significantly right after exercise and at the recovery stage to GPLC group. The serum FFA (free fatty acids) concentration by the administration of ergogenic aids (L-arginine, GPLC) had no statistically significant difference in every stage. Among fatigue substances, serum phosphorus concentration decreased significantly at each stage to L-arginine, and decreased significantly at the recovery stage to GPLC group. To GPLC group, there was significant reduction at the recovery stage. Ammonia concentration decreased significantly to L-arginine group more than GPLC group at each stage after administration compared to pre-administration. And there were significant differences before exercise and at the recovery stage to GPLC group. Lactate decreased significantly at each stage after administration to L-arginine and it significantly decreased before exercise and right after exercise to GPLC like in ammonia. In conclusion, administration of ergogenic aids(L-arginine, GPLC) resulted in the increase of nitrogen oxide concentration in the blood and concentration of fatigue substances such as Lactate, Ammonia and Phosphorus decreased. In terms of energy substrate, concentration of FFA(Free Fatty acids) in the blood showed higher value at exercise after administration than before administration, and concentration of glucose in the blood decreased.

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