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단일 근섬유 분리법(single fiber dissection)에 의한 12주 저항성 훈련 후 근육세포 내 완충단백질(carnosine, beta alanine, histidine)의 농도 변화 검증

The effect of 12-week resistance training on muscle buffering capacity by single fiber dissection

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김효정, 김세미, 김창근. 단일 근섬유 분리법에 의한 12주 저항성 훈련 후 근육세포 내 완충단백질의 농도 변화 검증. 운동과학, 제15권 제4호. 283-289, 2006. 이 연구는 저항성 훈련에 의한 근섬유 유형별 완충단백질의 농도 변화를 검증하기 위하여 수행되었다. 피험자(26-30세, n=5)를 대상으로 대퇴근육을 강화시킬 수 있는 운동 프로그램(squart, leg press, leg extension; 2 sessions/wk, 4 sets/exercise, 8RM/set)을 12주 동안 시행하였으며 트레이닝 전,후의 근생검(muscle biopsy) 조직에서 단일 근섬유(single fiber)를 분리하였다. 근세포 유형별 완충단백질의 농도 변화를 측정하기위해 MHC (myosin heavy chain)의 유형을 분석하는 SDS 전기영동을 시행하였으며 완충단백질의 농도는 HPLC로 분석하였다. 실험 결과 대퇴근육 면적이 증가(11%)되는 12주 저항성 트레이닝에 의해 carnosine(p<.01)과 taurine (p<.05)의 농도가 증가 하였으며 carnosine 합성 전구체인 beta -alanine이나 histidine 농도에는 변화가 없었다. 근섬유 유형별로 트레이닝 전 carnosine 농도는 type IIa (25.54mmolkg-1 DW)에서 type I (19.48mmolkg-1 DW) 보다 높은 것으로 나타났으며(p<.01) 트레이닝에 의한 type I 과 IIa의 증가량(type I, 8.12 mmolkg-1 DW; type IIa, 10.91 mmolkg-1 DW)은 거의 유사한 것으로 나타났다. 이상의 연구 결과는 저항성 트레이닝이 근육 내 완충단백질인 carnosine의 농도를 증가 시키는 효과적인 훈련 형태가 될 수 있으며 저항성 트레이닝에 의한 carnosine 농도 변화는 type I 과 IIa에서 모두 나타남을 시사하고 있다.

Abstract

Kim, H.J., Kim, S.M., Kim, C.K. The effect of 12-week resistance training on muscle buffering capacity by single fiber dissection. Exercise Science. 5(4): 283-289, 2006. The present study was performed to investigate the change of muscle buffering capacity with 12-week of resistance training. Five healthy volunteers (26-30yrs) participated in the present study. Percutaneous muscle biopsies and computed tomogram were obtained from the same portion of vastus lateralis muscle at pre- and post-resistance training. Carnosine content was analysed to muscle fiber type by single fiber dissection, MHC analysis, and HPLC technique. Training regimen was composed of three kinds of weight lifting exercise (squart, leg press, leg extension; 2 sessions/wk, 4 sets/exercise, 8RM/set) for 12weeks. Extracts of fibers or mixed muscle samples of biopsies were analysed for the content of carnosine, beta alanine, histidine and taurine by HPLC. Cross sectional area of the thigh muscle was 11% increased with training. Both carnosine and taurine content were significantly increased by 31%(p<.01) and 22% (p<.05), respectively. Carnosine, initially higher in type IIa fibres, increased in both type I(8.12 mmolkg-1 DW) and IIa(10.91 mmolkg-1 DW) fibres. No alteration was occurred in histidine and β-alanine content after 12 week of resistance training. In summary, the present study demonstrated that resistance exercise training associated with 11% increase of muscle CSA induced a qualitative increase of carnosine content, which is suggesting an increase in intracellular buffering capacity. The prospective of the present study supported the notion that power style resistance training may be more preferable measures to induce an increase in muscle buffering capacity.

참고문헌 (31)

  1. "Carnosine 보충 섭취가 운동시 생성되는 피로물질 및 운동수행 능력에 미치는 영향", 44, pp.253~360 (2004)
  2. "장기간 L- Carnosine 투여와 운동이 지질과산화 및 운동수행능력에 미치는 영향", 8(1), pp.79~85 (2004)
  3. "전문가를위한 최신 운동처방론" (1999), 체육과학연구원
  4. "Role of histidine-related compounds as intracellular proton buffering constituents in vertebrate muscle", 65, pp.757~765 (2000)
  5. "Influence of muscle fiber type and pedal frequency on oxygen uptake kinetics of heavy exercise", 81(4), pp.1642~1650 (1996)
  6. "Histidine- containing dipeptides as endogenous regulators of the activity of sarcoplasmic reticulum Ca-release channels", 1324, pp.142~150 (1997)
  7. "Acid-base balance during repeated bouts of exercise influence of HCO3", pp.228~231 (1984)
  8. "Role of taurine in osmoregulation during endurance exercise", 876, pp.489~495 (2002)
  9. "The carnosine C-2 proton's chemical shift reports intracellular pH in oxidative and glycolitic muscle fibers", 49, pp.233~240 (2003)
  10. "The significance of carnosine and anserine in striated skeletal muscle", pp.303~308 (1960), 한국체육대학교
  11. "The effects of three different training modalities on the cross-sectional area of the paravertebral muscles", 11, pp.335~341 (2001)
  12. "High-Performance liquid chromatographic determination of imidazole dipeptides, Histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibers", 688, pp.47~55 (1997)
  13. "Influence of oral β-alanine supplementation on carnosine content of gluteus medius", 30, pp.499~504 (1999)
  14. "Carnosine and taurine contents in individual fibres of human vastus lateralis muscles", 16, pp.639~643 (1998)
  15. "The absorption of orally supplied beta- alanine and its effect on muscle carnosine synthesis in human vastus lateralis", 30(3), pp.279~289 (2006)
  16. "Influence of beta-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity" (2006)
  17. "Effect on muscle fibre morphology and carnosine content after 12 days training of Korean speed skaters", 37(5) (2005)
  18. "Carnosine and anserine concentrations in the quadriceps femoris muscle of healthy humans", pp.47~50 (1992)
  19. "Decreased taurine concentration in skeletal muscles after exercise for various durations", 34(5), pp.793~797 (2002)
  20. "Buffering capacity of deprotenized human vastus lateralis muscle", pp.14~17 (1985)
  21. "Response of the trunk muscles to training assessed by magnetic resonance imaging and muscle strength", pp.383~387 (1992)
  22. "Serum amino acid responses to three different exercise sessions in male power", 42(4), pp.472~480 (2002)
  23. "Mechanisms underying taurine-mediated alterations in membrane function", 8, pp.231~246 (1995)
  24. "Esatimation of the carnosine content of different fibre types in the middle gluteal muscle of the throughbred horse", pp.447~453 (1992)
  25. "The effects of taurine on Ca2+ uptake by the sarcoplasmic reticulum and Ca2+ sensitivity of chemically skinned rat heart", pp.499~511 (1990)
  26. "High levels of skeletal muscle carnosine contributes to the later half of exercise performance during maximal cycle ergometer sprinting", 52, pp.199~205 (2002)
  27. "Fluid and carbohydrate replacement during intermittent exercise", 25, pp.157~172 (1998)
  28. "Effect of frog muscle phosphofructokinase", pp.4110~4114 (1966)
  29. "Metabolic correlates of fatigue from different types of exercise in man", 384, pp.185~194 (1995)
  30. "Purification of carnosine synthetase from avian muscle by affinity chromatography and determination of its subunit structure", pp.138~144 (1981)
  31. "Purification of carnosine synthetase from avian muscle by affinity chromatography and determination of its subunit structure", pp.138~144 (1981)

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