Effects of Acute Exercise on Mitochondrial Function, Dynamics, and Mitophagy in Rat Cardiac and Skeletal Muscles
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Purpose: This study aimed to investigate the effects of single-bout exercise on mitochondrial function, dynamics (fusion, fission), and mitophagy in cardiac and skeletal muscles. Methods: Fischer 344 rats (4 months old) were randomly divided into the control (CON) or acute exercise (EX) group (n=10 each). The rats performed a single bout of treadmill exercise for 60 minutes. Mitochondrial function (e.g., O2 respiration, H2O2 emission, Ca2+ retention capacity), mitochondrial fusion (e.g., Mfn1, Mfn2, Opa1), mitochondrial fission (e.g., Drp1, Fis1), and mitophagy (e.g., Parkin, Pink1, LC3II, Bnip3) were measured in permeabilized cardiac (e.g., left ventricle) and skeletal (e.g., soleus, white gastrocnemius) muscles. Results: Mitochondrial O2 respiration and Ca2+ retention capacity were significantly increased in all tissues of the EX group compared with the CON group. Mitochondrial H2O2 emissions showed tissue-specific results; the emissions showed no significant differences in the left ventricle or soleus (type I fibers) but was significantly increased in the white gastrocnemius (type II fibers) after acute exercise. Mitochondrial fusion and fission were not altered in any tissues of the EX group. Mitophagy showed tissue-specific differences: It was not changed in the left ventricle or white gastrocnemius, whereas Parkin and LC3II were significantly elevated in the soleus muscle. Conclusions: A single bout of aerobic exercise may improve mitochondrial function (e.g., O2 respiration and Ca2+ retention capacity) in the heart and skeletal muscles without changes in mitochondrial dynamics or mitophagy.
Abstract
Purpose: This study aimed to investigate the effects of single-bout exercise on mitochondrial function, dynamics (fusion, fission), and mitophagy in cardiac and skeletal muscles. Methods: Fischer 344 rats (4 months old) were randomly divided into the control (CON) or acute exercise (EX) group (n=10 each). The rats performed a single bout of treadmill exercise for 60 minutes. Mitochondrial function (e.g., O2 respiration, H2O2 emission, Ca2+ retention capacity), mitochondrial fusion (e.g., Mfn1, Mfn2, Opa1), mitochondrial fission (e.g., Drp1, Fis1), and mitophagy (e.g., Parkin, Pink1, LC3II, Bnip3) were measured in permeabilized cardiac (e.g., left ventricle) and skeletal (e.g., soleus, white gastrocnemius) muscles. Results: Mitochondrial O2 respiration and Ca2+ retention capacity were significantly increased in all tissues of the EX group compared with the CON group. Mitochondrial H2O2 emissions showed tissue-specific results; the emissions showed no significant differences in the left ventricle or soleus (type I fibers) but was significantly increased in the white gastrocnemius (type II fibers) after acute exercise. Mitochondrial fusion and fission were not altered in any tissues of the EX group. Mitophagy showed tissue-specific differences: It was not changed in the left ventricle or white gastrocnemius, whereas Parkin and LC3II were significantly elevated in the soleus muscle. Conclusions: A single bout of aerobic exercise may improve mitochondrial function (e.g., O2 respiration and Ca2+ retention capacity) in the heart and skeletal muscles without changes in mitochondrial dynamics or mitophagy.
참고문헌 (32)
- Petersen KF, "Mitochondrial dysfunction in the elderly : possible role in insulin resistance", Science, 300, pp.1140~1142 (2003)
- Montaigne D, "Myocardial contractile dysfunction is associated with impaired mitochondrial function and dynamics in type 2 diabetic but not in obese patients", Circulation, 130, pp.554~564 (2014)
- Anderson EJ, "Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways", Am J Physiol Heart Circ Physiol, 300, pp.H118~24 (2011)
- 유수지, "Role of exercise in age-related sarcopenia", Journal of exercise rehabilitation, 14(4), pp.551~558 (2018), 한국운동재활학회
- Weng SW, "The relationship between mitochondrial dynamics and insulin resistance in diabetes susceptible cybrid cells", Diabetes Res Clin Pract, 120, pp.S140~ (2016)
- Greene NP, "Mitochondrial quality control, promoted by PGC-1α, is dysregulated by Western diet-induced obesity and partially restored by moderate physical activity in mice", Physiol Rep, 3(7), pp.e12470~ (2015)
- Gram M, "Skeletal muscle mitochondrial H2O2 emission increases with immobilization and decreases after aerobic training in young and older men", J Physiol, 593, pp.4011~4027 (2015)
- Konopka AR, "Markers of human skeletal muscle mitochondrial biogenesis and quality control : effects of age and aerobic exercise training", J Gerontol A Biol Sci Med Sci, 69, pp.371~378 (2014)
- Tarpey MD, "Skeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat meal", Mol Metab, 6, pp.1597~1609 (2017)
- Kavazis AN, "Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli", Am J Physiol Heart Circ Physiol, 294, pp.H928~35 (2008)
- Zafaranieh S, "The effect of 12 weeks of aerobic exercise on mitochondrial dynamics in cardiac myocytes of type 2 diabetic rats", Sport Sci Health, 14, pp.305~312 (2018)
- Schefer V, "Oxygen consumption in adult and AGED C57BL", 6J mice during acute treadmill exercise of different intensity, 1996, pp.31, 387~392
- Ascensão A, "Acute exercise protects against calcium-induced cardiac mitochondrial permeability transition pore opening in doxorubicin-treated rats", Clin Sci, 120, pp.37~49 (2011)
- Fernström M, "Effects of acute and chronic endurance exercise on mitochondrial uncoupling in human skeletal muscle", J Physiol, 554, pp.755~763 (2004)
- Lee S, "Mitochondrial H2O2 generated from electron transport chain complex I stimulates muscle differentiation", Cell Res, 21, pp.817~834 (2011)
- 노미현, "Effects of aging on mitochondrial hydrogen peroxide emission and calcium retention capacity in rat heart", Journal of exercise rehabilitation, 14(6), pp.920~926 (2018), 한국운동재활학회
- Shadel GS, "Mitochondrial ROS signaling in organismal homeostasis", Cell, 163, pp.560~569 (2015)
- Smenes BT, "Acute exercise is not cardioprotective and may induce apoptotic signalling in heart surgery : a randomized controlled trial", Interact Cardiovasc Thorac Surg, 27, pp.95~101 (2018)
- Marín-García J, "The mitochondrial organelle and the heart", Rev Esp Cardiol, 55, pp.1293~1210 (2002)
- Park SY, "Cardiac, skeletal, and smooth muscle mitochondrial respiration: are all mitochondria created equal?", Am J Physiol Heart Circ Physiol, 307, pp.H346~52 (2014)
- Glancy B, "Protein composition and function of red and white skeletal muscle mitochondria", Am J Physiol Cell Physiol, 300, pp.C1280~90 (2011)
- Williams GS, "Mitochondrial calcium uptake", Proc Natl Acad Sci, 110, pp.10479~10486 (2013)
- Dirksen RT, "Sarcoplasmic reticulum-mitochondrial through-space coupling in skeletal muscle", Appl Physiol Nutr Metab, 34, pp.389~395 (2009)
- Picard M, "Acute exercise remodels mitochondrial membrane interactions in mouse skeletal muscle", J Appl Physiol, 115, pp.1562~1571 (2013)
- Iqbal S, "Oxidative stress-induced mitochondrial fragmentation and movement in skeletal muscle myoblasts", Am J Physiol Cell Physiol, 306, pp.C1176~83 (2014)
- Perry CG, "Repeated transient mRNA bursts precede increases in transcriptional and mitochondrial proteins during training in human skeletal muscle", J Physiol, 588, pp.4795~4710 (2010)
- Davies KJ, "Free radicals and tissue damage produced by exercise", Biochem Biophys Res Commun, 107, pp.1198~1205 (1982)
- Fan X, "H2O2-induced mitochondrial fragmentation in C2C12 myocytes", Free Radic Biol Med, 49, pp.1646~1654 (2010)
- Ding H, "Response of mitochondrial fusion and fission protein gene expression to exercise in rat skeletal muscle", Biochim Biophys Acta Gen Subj, 1800, pp.250~256 (2010)
- Narendra DP, "Targeting mitochondrial dysfunction : role for PINK1 and Parkin in mitochondrial quality control", Antioxid Redox Signal, 14, pp.1929~1938 (2011)
- Lo Verso F, "Autophagy is not required to sustain exercise and PRKAA1", AMPK activity but is important to prevent mitochondrial damage during physical activity, 2014, pp.10, 1883~1894
- Vainshtein A, "Role of PGC-1α during acute exercise-induced autophagy and mitophagy in skeletal muscle", Am J Physiol Cell Physiol, 308, pp.C710~9 (2015)