트레드밀 운동이 고지방식으로 유도된 알츠하이머 치매 마우스에서 인슐린 수용체, 산화 스트레스 및 시냅스 가소성 장애에 미치는 효과
The Effect of Treadmill Running on Insulin Receptor, Oxidative Stress, and Impaired Synaptic Plasticity in the Brain of High Fat Diet-induced 3xTg-AD Mice
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PURPOSE: This study investigated the effect of treadmill running on obesity-related brain insulin receptor, oxidative stress, and impaired synaptic plasticity in 3xTg-AD mice (APPSWE, PS1M146V and tauP301L). METHODS: At 4 months of age, thirty 3xTg-AD mice were assigned to standard chow (SC, n=10) or high-fat-diet (HFD, n=10) or HFD plus exercise training (HFD+EX, n=10). HFD+EX mice were subjected to treadmill running at a moderate intensity with duration of 30 minutes per day and frequency of 5 days per week for 20 weeks. HFD mice were fed a 60% fat HFD for the same period. Mice were sacrificed and histological and western blot analysis was performed. RESULTS: Compared with the SC mice, the HFD mice had significantly lower levels of p-IRβ/IRβ (p<.05) in conjunction with APP (p<.05), Aβ oligomer (p<.05), HNE/NEUN (p<.05), PSD95 (p<.05), DCX/NeuN (p<.05) in the hippocampus. On the other hand, we found that treadmill running attenuated HFD-induced exacerbations of APP (p<.05), Aβ oligomer (p<.05), PSD95 (p<.05), DCX/ NeuN (p<.05) in the hippocampus. CONCLUSIONS: The current findings suggest that treadmill running protects against AD-like disease progression and impaired synaptic plasticity, cell proliferation caused by a HFD in the 3xTg-AD mice.
Abstract
PURPOSE: This study investigated the effect of treadmill running on obesity-related brain insulin receptor, oxidative stress, and impaired synaptic plasticity in 3xTg-AD mice (APPSWE, PS1M146V and tauP301L). METHODS: At 4 months of age, thirty 3xTg-AD mice were assigned to standard chow (SC, n=10) or high-fat-diet (HFD, n=10) or HFD plus exercise training (HFD+EX, n=10). HFD+EX mice were subjected to treadmill running at a moderate intensity with duration of 30 minutes per day and frequency of 5 days per week for 20 weeks. HFD mice were fed a 60% fat HFD for the same period. Mice were sacrificed and histological and western blot analysis was performed. RESULTS: Compared with the SC mice, the HFD mice had significantly lower levels of p-IRβ/IRβ (p<.05) in conjunction with APP (p<.05), Aβ oligomer (p<.05), HNE/NEUN (p<.05), PSD95 (p<.05), DCX/NeuN (p<.05) in the hippocampus. On the other hand, we found that treadmill running attenuated HFD-induced exacerbations of APP (p<.05), Aβ oligomer (p<.05), PSD95 (p<.05), DCX/ NeuN (p<.05) in the hippocampus. CONCLUSIONS: The current findings suggest that treadmill running protects against AD-like disease progression and impaired synaptic plasticity, cell proliferation caused by a HFD in the 3xTg-AD mice.
참고문헌 (49)
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