트레드밀 지구성 운동이 알츠하이머 형질전환 생쥐 뇌의 mitochondrial dynamic과 신경세포생존에 미치는 영향
The effect of treadmill exercise on mitochondrial dynamic function and neuronal cell survival in transgenic mice model of Alzheimer's disease.
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PURPOSE: The aim of this study is to investigate the effects of treadmill exercise on mitochondrial Aβprotein, mitochondrial dynamic function and neuronal cell survival of brain cortex mitochondria in transgenic mice model of Alzheimer's disease METHODS: Fifteen transgenic mice of Alzheimer's disease were divided into three groups: (1) Non Tg control(NTC, n=5), (2) Tg control(TC, n=5) and (3) Tg-exercise(TE, n=5). TE mice group was subjected to treadmill exercise on a treadmill for 12 weeks(15m/min, 60min/day, 5day/week). And then, brain cortex mitochondria were evaluated to determine whether any changes in the Aβ-42, neuronal cell survival-related proteins, Sirt-3, SOD-2, mitochondrial dynamic-related proteins. RESULTS: Our results demonstrated that treadmill exercise decreased Aβ-42 and mitochondiral fission proteins(Drp-1, Fis-1) in the brain cortex mitochondria. In addition, treadmill exercise increased Sirt-3, SOD-2 and mitochondrial fusion proteins(Mfn-1, Mfn-2, Opa-1) and neuronal cell survival factors(Bcl-2/Bax ratio, mito-cytochrome c/ cyto--cytochrome c ratio) in the brain cortex mitochondria. Moreover, treadmill exercise improved spatial learning and cognitive function in TE group. CONCLUSIONS: Taken together, these results suggest that treadmill exercise protect against Aβ-induced imbalance of mitochondrial dynamic function and neuronal cell death through decreasing Aβprotein and mitochondria fission and activating mitochondrial fusion, indicating that treadmill exercise may play a role in the preventive strategy for AD-related mitochondrial dysfunction.
Abstract
PURPOSE: The aim of this study is to investigate the effects of treadmill exercise on mitochondrial Aβprotein, mitochondrial dynamic function and neuronal cell survival of brain cortex mitochondria in transgenic mice model of Alzheimer's disease METHODS: Fifteen transgenic mice of Alzheimer's disease were divided into three groups: (1) Non Tg control(NTC, n=5), (2) Tg control(TC, n=5) and (3) Tg-exercise(TE, n=5). TE mice group was subjected to treadmill exercise on a treadmill for 12 weeks(15m/min, 60min/day, 5day/week). And then, brain cortex mitochondria were evaluated to determine whether any changes in the Aβ-42, neuronal cell survival-related proteins, Sirt-3, SOD-2, mitochondrial dynamic-related proteins. RESULTS: Our results demonstrated that treadmill exercise decreased Aβ-42 and mitochondiral fission proteins(Drp-1, Fis-1) in the brain cortex mitochondria. In addition, treadmill exercise increased Sirt-3, SOD-2 and mitochondrial fusion proteins(Mfn-1, Mfn-2, Opa-1) and neuronal cell survival factors(Bcl-2/Bax ratio, mito-cytochrome c/ cyto--cytochrome c ratio) in the brain cortex mitochondria. Moreover, treadmill exercise improved spatial learning and cognitive function in TE group. CONCLUSIONS: Taken together, these results suggest that treadmill exercise protect against Aβ-induced imbalance of mitochondrial dynamic function and neuronal cell death through decreasing Aβprotein and mitochondria fission and activating mitochondrial fusion, indicating that treadmill exercise may play a role in the preventive strategy for AD-related mitochondrial dysfunction.
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