목향 추출물의 항산화 및 항암활성
Antioxidative and Antitumor Activity of Extracts from Saussurea lappa
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초록
This study was carried out to investigate the antioxidative and antitumor activities of Saussurea lappa for the purpose of developing a functional food. The methanol extract of Saussurea lappa was fractionated with five solvents(hexane, chloroform, EtOAc, BuOH, water) and examined antioxidative activities and xanthine oxidase inhibitory activity in addition to growth inhibitory activity of human cancer cell(HT-29, SNU-1, HeLa). Total phenol compounds contents were higher in EtOAC fraction than other fractions. Also, electron donating ability was over 90% at 500㎍/㎖ (93.1%) and 1000㎍/㎖ (94.3%). Hexane fraction revealed stronger nitrite scavenging ability than positive control(ascorbic acid) and its abilities were 22.4% and 42.8% at 500㎍/㎖ and 1000㎍/㎖, respectively. Xanthine oxidase inhibitory activity had similar tendency to electron donating ability. EtOAc fraction showed high inhibition to xanthien oxidase activities at 500㎍/㎖ (81.9%) and 1000㎍/㎖ (90.4%). In antitumor activity test, hexane fraction exhibited potent growth inhibition activity against HT-29, SNU-1 and HeLa cells. Therefore, we believe that Saussurea lappa can be developed into functional food with antioxidant activity. Additional studies are required for the hexane and chloroform fractions of Saussurea lappa to develop them into therapeutic supplements for stomach cancer, colon cancer, and cervical cancer.
Abstract
This study was carried out to investigate the antioxidative and antitumor activities of Saussurea lappa for the purpose of developing a functional food. The methanol extract of Saussurea lappa was fractionated with five solvents(hexane, chloroform, EtOAc, BuOH, water) and examined antioxidative activities and xanthine oxidase inhibitory activity in addition to growth inhibitory activity of human cancer cell(HT-29, SNU-1, HeLa). Total phenol compounds contents were higher in EtOAC fraction than other fractions. Also, electron donating ability was over 90% at 500㎍/㎖ (93.1%) and 1000㎍/㎖ (94.3%). Hexane fraction revealed stronger nitrite scavenging ability than positive control(ascorbic acid) and its abilities were 22.4% and 42.8% at 500㎍/㎖ and 1000㎍/㎖, respectively. Xanthine oxidase inhibitory activity had similar tendency to electron donating ability. EtOAc fraction showed high inhibition to xanthien oxidase activities at 500㎍/㎖ (81.9%) and 1000㎍/㎖ (90.4%). In antitumor activity test, hexane fraction exhibited potent growth inhibition activity against HT-29, SNU-1 and HeLa cells. Therefore, we believe that Saussurea lappa can be developed into functional food with antioxidant activity. Additional studies are required for the hexane and chloroform fractions of Saussurea lappa to develop them into therapeutic supplements for stomach cancer, colon cancer, and cervical cancer.
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