참나물의 항균, 항산화 활성 및 대장암세포 성장억제 활성 평가
Antibacterial, Antioxidative and Anti-proliferative Activity against Human Colorectal Cell of Pimpinella brachycarpa
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초록
Pimpinella brachycarpa, called as cham-na-mul in Korea, is an edible popular herb. However, the study of biological activity of P. brachycarpa is still rudimentary in worldwide. In this study, from the cultivated P. brachycarpa, we prepared the methanol extract and its subsequent solvent fractions, and their antimicrobial, antioxidation, and anti-proliferative activities were evaluated. The fraction yields of n-hexane (H), methylene chloride (EC), ethylacetate (EA), butanol (B), and water residue (W) from the methanol extract were 18.71, 0.7, 0.56, 4.57, and 71.51%, respectively. Analysis of total flavonoid and total polyphenol showed that the EA fraction contained the highest contents (89.23 and 200 mg/g), and the W residue has the lowest contents (19.6 and 2.27 mg/g) among the factions. In antimicrobial activity assay, the EA fraction showed a broad-range antibacterial activity, while the H fraction is effective against gram positive bacteria. In antioxidation activity assay, EA and B fraction showed strong DPPH anion and ABTS cation scavenging activities including reducing power, and H and MC fraction showed effective nitrite scavenging activity (71.43∼83.82 μg/mL of IC50). In a while, only B fraction showed strong anti-proliferative activity against human colorectal cancer HCT-116 (166 μg/mL of IC50) as a dose-dependent manner up to 200 μg/mL. These results suggest that the EA and B fraction of P. brachycarpa could be developed as functional food ingredients.
Abstract
Pimpinella brachycarpa, called as cham-na-mul in Korea, is an edible popular herb. However, the study of biological activity of P. brachycarpa is still rudimentary in worldwide. In this study, from the cultivated P. brachycarpa, we prepared the methanol extract and its subsequent solvent fractions, and their antimicrobial, antioxidation, and anti-proliferative activities were evaluated. The fraction yields of n-hexane (H), methylene chloride (EC), ethylacetate (EA), butanol (B), and water residue (W) from the methanol extract were 18.71, 0.7, 0.56, 4.57, and 71.51%, respectively. Analysis of total flavonoid and total polyphenol showed that the EA fraction contained the highest contents (89.23 and 200 mg/g), and the W residue has the lowest contents (19.6 and 2.27 mg/g) among the factions. In antimicrobial activity assay, the EA fraction showed a broad-range antibacterial activity, while the H fraction is effective against gram positive bacteria. In antioxidation activity assay, EA and B fraction showed strong DPPH anion and ABTS cation scavenging activities including reducing power, and H and MC fraction showed effective nitrite scavenging activity (71.43∼83.82 μg/mL of IC50). In a while, only B fraction showed strong anti-proliferative activity against human colorectal cancer HCT-116 (166 μg/mL of IC50) as a dose-dependent manner up to 200 μg/mL. These results suggest that the EA and B fraction of P. brachycarpa could be developed as functional food ingredients.
참고문헌 (25)
- 장경미, "참나물 첨가 기능성식품 개발을 위한 조리과학적 연구", 한국식생활문화학회지, 22(2), pp.274~282 (2007), 한국식생활문화학회
- 최미희, "숙성 온도를 달리한 참나물 김치의 품질특성 조사", 한국식품영양과학회지, 31(1), pp.45~49 (2002), 한국식품영양과학회
- Choi NS, "Change of biologically functional compounds of Pimpinella brachycarpa (Chamnamul) by blanching conditions", Kor J Dietary Culture, 16, pp.388~397 (2001)
- 장경미, "ICP-AES와 SDE, HS-SPME-GC", MS를 이용한 참나물의 무기성분과 향기성분, 2007, pp.22(2), 246~253[DOI]
- 이명렬, "참나물의 이화학적 성분", 한국식품영양과학회지, 36(3), pp.327~331 (2007), 한국식품영양과학회
- 김준철, "Isolation of Callus-specific mRNAs from Differentiating Embryogenic Somatic Calli of Pimpinella brachycarpa by cDNA-AFLP", Molecules and Cells, 17(1), pp.39~44 (2004), 한국분자세포생물학회
- Son SI, "Cloning and characterization of homeodomain-zip gene, Phc5 in embryogenic callus derived from Pimpinella brachycarpa suspension cultured cells", Kor J Plant Tissue Culture, 26, pp.121~126 (1999)
- Cha YR, "Cloning of a cDNA encoding phospholipase D from Pimpinella brachycarpa", MOLECULES AND CELLS, 8(1), pp.19~26, SPRINGER-VERLAG SINGAPORE PTE LTD
- Lee MK, "The effect of far infrared ray-vacuum drying on the quality changes of Pimpinella brachycarpa", J Kor Soc Food Sci Nutr, 29, pp.561~567 (2000)
- Song HS, "Usefulness of Pimpinella brachycarpa as natural spice by sensory analysis", Kor J Food Sci, 13, pp.669~673 (1997)
- 이명렬, "참나물 에탄올 추출물의 항산화 효과", 생명과학회지, 18(4), pp.467~473 (2008), 한국생명과학회
- 이명렬, "참나물이 만성적으로 알코올 유도된 흰쥐의 간손상에 미치는 영향", 생명과학회지, 17(10), pp.1406~1413 (2007), 한국생명과학회
- 이명렬, "참나물이 고콜레스테롤식이를 섭취한 흰쥐의 지질대사에 미치는 영향", 한국식품영양과학회지, 35(9), pp.1151~1158 (2006), 한국식품영양과학회
- 권정숙, "식용 및 약용 산채류로부터 트롬빈 저해물질의 탐색", 생명과학회지, 14(3), pp.509~513 (2004), 한국생명과학회
- 김지인, "마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가", 한국미생물·생명공학회지, 37(2), pp.133~139 (2009), 한국미생물·생명공학회
- 안선미, "체리(Prunus avium L.)의 항균 및 항산화 활성", 한국미생물·생명공학회지, 37(4), pp.371~376 (2009), 한국미생물·생명공학회
- 손건호, "대파(Allium fistulosum L)로부터 fistulosides의 분리와 분리 물질의 항진균 활성", 생명과학회지, 16(2), pp.310~314 (2006), 한국생명과학회
- 손호용, "재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성", 생명과학회지, 16(4), pp.647~652 (2006), 한국생명과학회
- 손호용, "Candidiosis 치료제 개발을 위한 약용 및 야생 식물의 항진균 활성의 검색", 생명과학회지, 13(5), pp.604~617 (2003), 한국생명과학회
- 류희영, "한약재의 항균 활성 및 인간 적혈구 용혈 활성", 한국미생물·생명공학회지, 38(2), pp.190~197 (2010), 한국미생물·생명공학회
- 안선미, "해조류 추출물의 항산화 및 nitrite 소거활성 평가", 생명과학회지, 21(4), pp.576~583 (2011), 한국생명과학회
- 손호용, "장수상황버섯 균사체를 이용한 고체 발효한약재의 대장암 세포성장 억제 활성", 생명과학회지, 20(8), pp.1268~1275 (2010), 한국생명과학회
- Sohn HY, "Induction of oxidative stress by endosulfan and protective effect of lipid-soluble antioxidants against endosulfan- induced oxidative damage", Toxicol Lett, 151, pp.357~365 (2004)
- 류희영, "항생제 다제내성 Pseudomonas aeruginosa 및 Candida균주에 대한 산사자의 항균 활성", 한국미생물·생명공학회지, 38(1), pp.77~83 (2010), 한국미생물·생명공학회
- Sohn HY, "Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai", PHYTOMEDICINE, 11, pp.666~672, ELSEVIER GMBH