애엽(艾葉) 에탄올 및 메탄올 추출물과 용매별 분획의 세포독성과 항산화활성
Cytotoxicity and Antioxidative Activity of Artemisiae Argi Folium Alcoholic Extracts and Their Fractions
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This experiment was carried out to obtain the cytotoxicity and antioxidative activity of Artemisiae Argi Folium. The total polyphenol contents in the ethyl acetate fraction of the ethanol extract and the methanol extract were 430.27㎎/g and 427.84㎎/g, respectively. In DPPH radical scavenging ability, SC_50 values of the ethyl acetate fraction of the ethanol extract and the methanol extract were 32.64 ㎍/㎖ and 27.70 ㎍/㎖ as the same level of statistical with ascorbic acid. In the cytotoxicity measurement by MTT assay, the chloroform and hexane fraction, and each extract were exhibited higher cytotoxicity than the other fractions. In particular, the ethyl acetate fractions appeared high activity in DPPH radical scavenging ability were began to show cytotoxicity in 125 ㎍/㎖. As a result, the ethyl acetate fraction of Artemisiae Argi Folium extract was the most highly active fraction in antioxidative activity. However, for the use of extracts and fractions from Artemisiae Argi Folium to related fields, the setting of appropriate concentration is required.
Abstract
This experiment was carried out to obtain the cytotoxicity and antioxidative activity of Artemisiae Argi Folium. The total polyphenol contents in the ethyl acetate fraction of the ethanol extract and the methanol extract were 430.27㎎/g and 427.84㎎/g, respectively. In DPPH radical scavenging ability, SC_50 values of the ethyl acetate fraction of the ethanol extract and the methanol extract were 32.64 ㎍/㎖ and 27.70 ㎍/㎖ as the same level of statistical with ascorbic acid. In the cytotoxicity measurement by MTT assay, the chloroform and hexane fraction, and each extract were exhibited higher cytotoxicity than the other fractions. In particular, the ethyl acetate fractions appeared high activity in DPPH radical scavenging ability were began to show cytotoxicity in 125 ㎍/㎖. As a result, the ethyl acetate fraction of Artemisiae Argi Folium extract was the most highly active fraction in antioxidative activity. However, for the use of extracts and fractions from Artemisiae Argi Folium to related fields, the setting of appropriate concentration is required.
참고문헌 (25)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Branen AL, "Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene", Journal of the American Oil Chemists' Society, 52, pp.59~65 (1975)
- 최소라, "사철쑥의 항산화성과 항균성", 한국약용작물학회지, 16(2), pp.112~117 (2008), 한국약용작물학회
- 최소라, "이용부위 및 채취시기에 따른 꾸지뽕나무 메탄올 추출물의 항산화성", 한국약용작물학회지, 17(2), pp.115~120 (2009), 한국약용작물학회
- 엄효진, "건조온도에 따른 비파 잎의 생리활성 변화", 생약학회지, 40(3), pp.178~183 (2009), 한국생약학회
- Farag RS, "Influence of thyme and clove essential oils in cotton seed oil oxidation", Journal of the American Oil Chemists' Society, 66, pp.800~806 (1989)
- Fridovich I, "Superoxide dismutase an adaption to paramagnetic gas", Journal of Biological Chemistry, 264, pp.7761~7762 (1989)
- 임도연, "흰민들레 에탄올 추출물 및 분획물의 항산화 및 항균활성과 Tyrosinase 저해활성", 한국약용작물학회지, 19(4), pp.238~245 (2011), 한국약용작물학회
- 김홍태, "인진쑥 추출물의 세포독성 효과", 한국임상수의학회지, 24(3), pp.367~371 (2007), 한국임상수의학회
- 이현화, "흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교", 한국약용작물학회지, 16(2), pp.79~85 (2008), 한국약용작물학회
- 이경인, "산벚나무 수피 추출물 및 용매 분획물의 여드름 원인균에 대한 항균활성과 Tyrosinase 저해 활성", 생약학회지, 42(2), pp.155~160 (2011), 한국생약학회
- 이미경, "사철쑥 추출물의 면역세포의 생육증진 및 세포독성", 한국약용작물학회지, 12(1), pp.36~42 (2004), 한국약용작물학회
- 이승민, "애엽 추출물이 골 대사에 미치는 영향", 한국식품영양과학회지, 39(3), pp.363~368 (2010), 한국식품영양과학회
- Liu RH, "Potential synergy of phytochemicals in cancer prevention : mechanism of action", The Journal of Nutrition, 134, pp.3479S~3485S (2004)
- Manach C, "Bioavailability and bioefficacy of polyphenols in humans", The American Journal of Clinical Nutrition, 81, pp.230S~242S (2005)
- Miller Y, "Toxicity of some essential plant oils", Clinical Toxicology, 8, pp.1485~1487 (1981)
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several region of Argentina", Journal of Ethnopharmacology, 71, pp.109~114 (2000)
- 오영지, "천궁(Cnidium officinale Makino) 지상부 추출물의 항산화 활성 평가", 한국약용작물학회지, 18(6), pp.373~378 (2010), 한국약용작물학회
- 박완수, "마우스 대식세포(Raw 264.7)에 대한 艾葉 물추출물의 생리활성 연구", 동의생리병리학회지, 22(4), pp.815~820 (2008), 대한동의병리학회
- 박완수, "Lipopolysaccharide로 활성화된 마우스 대식세포에서 艾葉 물추출물의 면역활성 연구", 대한본초학회지, 24(1), pp.151~157 (2009), 대한본초학회
- Qureshi S, "Preliminary toxicity studies on ethanol extracts of the aerial parts of Artemisia abyssinica and A", inculta in mice. Journal of Ethnopharmacology, 28, pp.157~162 (1990)
- 유민정, "6종의 약용식물 추출물의 비듬균(Malassezia furfur)에 대한 증식억제 및 항산화 효과", 한국미용학회지, 16(1), pp.120~128 (2010), 한국미용학회
- 신경민, "Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과", 생약학회지, 34(3), pp.223~227 (2003), 한국생약학회
- Videla LA, "Biochemical aspects of cellular oxidative stress", Archivos de biología y medicina experimentales, 21, pp.85~92 (1988)
- 박수영, "큰비쑥(Artemisia fukudo) 추출물의 murine macrophage RAW", 한국식품과학회지, 39(4), pp.464~469 (2007), 한국식품과학회