FI-HG-AAS를 이용한 전처리 과정에서 사용되는 예비환원제의 종류에 따른 요중 비소 분석결과 비교
Comparisons of Urinary Arsenic Analysis by Pre-reductant for Preconditioning via the FI-HG-AAS Method
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Objectives: The method of analyzing urinary arsenic by flow injection hydride generation atomicabsorption spectrometry (FI-HG-AAS) is generally used because it shows relatively greater sensitivity, lowdetection limits, low blocking action, and is simple to operate. In this study, the results of analysisaccording to three pre-reductants commonly used in the FI-HG-AAS method were compared with eachother. Methods: To analyze urinary arsenic, nineteen urine samples were collected from adults aged 43-79 years oldwithout occupational arsenic exposure. Analysis equipment was FI-HG-AAS (AAnalyst 800/FIAS 400, Perkin-Elmer Inc., USA). The three pre-reductants were potassium iodide (KI/AA), C3H7NO2S (L-cysteine), and amixture of KI/AA and L-cysteine (KI/AA&L-cysteine). Results: In the results of the analysis, the recovery rate of the method using KI/AA was 82.3%, 95.7% for Lcysteine,and 123.5% for KI/AA and L-cysteine combined. When compared with the results by use of highperformance liquid chromatography inductively-coupled plasma mass spectrometry (HPLC-ICP-MS), themethod using L-cysteine was the closest to those using HPLC-ICP-MS (98.57 μg/L for HPLC-ICP-MS; 74.96μg/L for L-cysteine; 69.23 μg/L for KI/AA and L-cysteine; 13.06 μg/L for KI/AA) and were significantlycorrelated (R2=0.882). In addition, they showed the lowest coefficient of variation in the results between twolaboratories that applied the same method. Conclusion: The efficiency of hydride generation is considered highly important to the analysis of urinaryarsenic via FI-HG-AAS. This study suggests that using L-cysteine as a pre-reductant may be suitable and themost rational among the FI-Hg-AAS methods using pre-reductants.
Abstract
Objectives: The method of analyzing urinary arsenic by flow injection hydride generation atomicabsorption spectrometry (FI-HG-AAS) is generally used because it shows relatively greater sensitivity, lowdetection limits, low blocking action, and is simple to operate. In this study, the results of analysisaccording to three pre-reductants commonly used in the FI-HG-AAS method were compared with eachother. Methods: To analyze urinary arsenic, nineteen urine samples were collected from adults aged 43-79 years oldwithout occupational arsenic exposure. Analysis equipment was FI-HG-AAS (AAnalyst 800/FIAS 400, Perkin-Elmer Inc., USA). The three pre-reductants were potassium iodide (KI/AA), C3H7NO2S (L-cysteine), and amixture of KI/AA and L-cysteine (KI/AA&L-cysteine). Results: In the results of the analysis, the recovery rate of the method using KI/AA was 82.3%, 95.7% for Lcysteine,and 123.5% for KI/AA and L-cysteine combined. When compared with the results by use of highperformance liquid chromatography inductively-coupled plasma mass spectrometry (HPLC-ICP-MS), themethod using L-cysteine was the closest to those using HPLC-ICP-MS (98.57 μg/L for HPLC-ICP-MS; 74.96μg/L for L-cysteine; 69.23 μg/L for KI/AA and L-cysteine; 13.06 μg/L for KI/AA) and were significantlycorrelated (R2=0.882). In addition, they showed the lowest coefficient of variation in the results between twolaboratories that applied the same method. Conclusion: The efficiency of hydride generation is considered highly important to the analysis of urinaryarsenic via FI-HG-AAS. This study suggests that using L-cysteine as a pre-reductant may be suitable and themost rational among the FI-Hg-AAS methods using pre-reductants.
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