임플란트 적용을 위한 하이드록시아파타이트·이산화티탄 표면의 생체적합성 평가
A Biocompatibility Evaluation of Hydroxyapaite·Titania Surface for Dental Implant
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The objective of this study was to fabricate hydroxyapatite (HA) containing titania layer by HA blasting and anodization method to obtain advantagesof both methods and evaluated biocompatibility. To fabricate the HA containing titania layer on titanium, HA blasting treatment was performedfollowed by microarc oxidation (MAO) using the electrolyte solution of 0.04 M -glycerol phosphate disodium salt n-hydrate and 0.4 M calciumacetate n-hydrate on the condition of various applied voltages (100, 150, 200, 250 V) for 3 minutes. The experimental group was divided accordingto the surface treatment procedure: SM (simple machined polishing treatment), HA, MAO, HA＋MAO 100, HA＋MAO 150, HA＋MAO 200, HA＋MAO 250. The wettability of surface was observed by contact angle measurement. Biocompatibility was evaluated by cell adhesion, and celldifferentiation including alkaline phosphatase activity and calcium concentration with MC3T3-E1 cells. The porous titanium oxide containing HA wasformed at 150 and 200 V. These surfaces had a more hydrophilic characteristic. Biocompatibility was demonstrated that HA·titania compositelayer on titanium showed enhanced cell adhesion, and cell differentiation. Therefore, these results suggested that HA containing titania layer ontitanium was improved biological properties that could be applied as material for dental implant system.
Abstract
The objective of this study was to fabricate hydroxyapatite (HA) containing titania layer by HA blasting and anodization method to obtain advantagesof both methods and evaluated biocompatibility. To fabricate the HA containing titania layer on titanium, HA blasting treatment was performedfollowed by microarc oxidation (MAO) using the electrolyte solution of 0.04 M -glycerol phosphate disodium salt n-hydrate and 0.4 M calciumacetate n-hydrate on the condition of various applied voltages (100, 150, 200, 250 V) for 3 minutes. The experimental group was divided accordingto the surface treatment procedure: SM (simple machined polishing treatment), HA, MAO, HA＋MAO 100, HA＋MAO 150, HA＋MAO 200, HA＋MAO 250. The wettability of surface was observed by contact angle measurement. Biocompatibility was evaluated by cell adhesion, and celldifferentiation including alkaline phosphatase activity and calcium concentration with MC3T3-E1 cells. The porous titanium oxide containing HA wasformed at 150 and 200 V. These surfaces had a more hydrophilic characteristic. Biocompatibility was demonstrated that HA·titania compositelayer on titanium showed enhanced cell adhesion, and cell differentiation. Therefore, these results suggested that HA containing titania layer ontitanium was improved biological properties that could be applied as material for dental implant system.
참고문헌 (27)
- 한지형, "임플란트 시술환자 시술 전·후의 기대와 만족도 비교", 치위생과학회지, 11(2), pp.121~127 (2011), 한국치위생과학회
- Liu X, "Surface modification of titanium, titanium alloys, and related materials for biomedical applications", Mater Sci Eng R-Rep, 47, pp.49~121 (2004)
- Coelho PG, "Basic research methods and current trends of dental implant surfaces", J Biomed Mater Res B Appl Biomater, 88, pp.579~596 (2009)
- Le Guéhennec L, "Surface treatments of titanium dental implants for rapid osseointegration", Dent Mater, 23, pp.844~854 (2007)
- Ishikawa K, "Blast coating method: new method of coating titanium surface with hydroxyapatite at room temperature", J Biomed Mater Res, 38, pp.129~134 (1997)
- 강민경, "치과용 임플란트 적용을 위한 항균력을 가진 티타늄 표면의 평가", 치위생과학회지, 11(5), pp.405~410 (2011), 한국치위생과학회
- O'Hare P, "Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique", Biomaterials, 31, pp.515~522 (2010)
- Ishizawa H, "Formation and characterization of anodic titanium oxide films containing Ca and P", J Biomed Mater Res, 29, pp.65~72 (1995)
- Yang B, "Preparation of bioactive titanium metal via anodic oxidation treatment", Biomaterials, 25, pp.1003~1010 (2004)
- Ishizawa H, "Characterization of thin hydroxyapatite layers formed on anodic titanium oxide films containing Ca and P by hydrothermal treatment", J Biomed Mater Res, 29, pp.1071~1079 (1995)
- Kang MK, "Characterization of hydroxyapatite containing a titania layer formed by anodization coupled with blasting", Acta Odontol Scand, 72, pp.989~998 (2014)
- Elias CN, "Relationship between surface properties (roughness, wettability and morphology)of titanium and dental implant removal torque", J Mech Behav Biomed Mater, 1, pp.234~242 (2008)
- Bico J, "Wetting of textured surfaces", Colloids Surf A, 206, pp.41~46 (2002)
- Zhu X, "Effects of topography and composition of titanium surface oxides on osteoblast responses", Biomaterials, 25, pp.4087~4103 (2004)
- Park JW, "Osteoconductivity of hydrophilic microstructured titanium implants with phosphate ion chemistry", Acta Biomater, 5, pp.2311~2321 (2009)
- Macak JM, "TiO2 nanotubes:self-organized electrochemical formation, properties and applications", Curr Opin Solid State Mat Sci, 11, pp.3~18 (2007)
- Anselme K, "Osteoblast adhesion on biomaterials", Biomaterials, 21, pp.667~681 (2000)
- Beck GR, "Inorganic phosphate as a signaling molecule in osteoblast differentiation", J Cell Biochem, 90, pp.234~243 (2003)
- Kieswetter K, "Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells", J Biomed Mater Res, 32, pp.55~63 (1996)
- Boyan BD, "Titanium surface roughness alters responsiveness of MG63 osteoblast-like cells to 1α,25-(OH)2D3", J Biomed Mater Res, 39, pp.77~85 (1998)
- 이상임, "The Role of NFATc1 on Osteoblastic Differentiation in Human Periodontal Ligament Cells", 치위생과학회지, 15(4), pp.488~494 (2015), 한국치위생과학회
- Giordano C, "A new chemical etching process to improve endosseous implant osseointegration:In vitro evaluation on human osteoblast-like cells", Int J Artif Organs, 29, pp.772~780 (2006)
- Le Guehennec L, "Osteoblastic cell behaviour on different titanium implant surfaces", Acta Biomater, 4, pp.535~543 (2008)
- Lian JB, "Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation", Crit Rev Oral Biol Med, 3, pp.269~305 (1992)
- Beck GR, "Inorganic phosphate as a signaling molecule in osteoblast differentiation", J Cell Biochem, 90, pp.234~243 (2003)
- Anderson H, "Matrix vesicles and calcification", Curr Rheumatol Rep, 5, pp.222~226 (2003)
- Chehroudi B, "The effects of micromachined surfaces on formation of bonelike tissue on subcutaneous implants as assessed by radiography and computer image processing", J Biomed Mater Res, 34, pp.279~290 (1997)