티타늄 표면처리가 골모세포의 반응에 미치는 영향
Effect of titanium surface treatment on reaction of osteoblast cells
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Purpose: This study was carried out to evaluate the effects of several surface modifications with titanium on cell responses. Materials and Methods: Titanium discs (15 mm in diameter and 2 mm in thickness) were divided into five groups; control group, acid-treated group, alkali-heat-treated group, sandblasting with large grit and acid etching group, and resorbable blasted media group. The control group had a non-treated machined titanium surface. Changes in surface morphology were observed by scanning electron microscope (SEM) and atomic force microscope, substances were analyzed by X-ray diffraction, and hydrophilic properties and surface tension were measured by the contact angle test. In order to examine biological responses, SEM observation, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and measurement of alkaline phosphatase (ALP) activity were carried out. Results: The results are as follows. Each group showed a different surface morphology. Roughness test showed a statistically significant difference in Ra values. In the SEM analysis, spindle shaped cells with well-developed projections were detected, and cells were well attached. The alkali-heat-treated group showed the greatest number of attached cells, which had connecting projections. In the MTT analysis, the alkali-heat-treated group showed better cell viability than the other groups. Control and alkali-heat-treated groups showed similar cell viabilities. In the ALP activity test, every surface-treated group showed significantly higher values than the control group. Especially, alkali-heat-treated group had the highest ALP activity. Conclusion: The results suggest that titanium surface modification could show better surface properties and biological characteristics, especially in the alkali-heat-treated surface.
Abstract
Purpose: This study was carried out to evaluate the effects of several surface modifications with titanium on cell responses. Materials and Methods: Titanium discs (15 mm in diameter and 2 mm in thickness) were divided into five groups; control group, acid-treated group, alkali-heat-treated group, sandblasting with large grit and acid etching group, and resorbable blasted media group. The control group had a non-treated machined titanium surface. Changes in surface morphology were observed by scanning electron microscope (SEM) and atomic force microscope, substances were analyzed by X-ray diffraction, and hydrophilic properties and surface tension were measured by the contact angle test. In order to examine biological responses, SEM observation, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and measurement of alkaline phosphatase (ALP) activity were carried out. Results: The results are as follows. Each group showed a different surface morphology. Roughness test showed a statistically significant difference in Ra values. In the SEM analysis, spindle shaped cells with well-developed projections were detected, and cells were well attached. The alkali-heat-treated group showed the greatest number of attached cells, which had connecting projections. In the MTT analysis, the alkali-heat-treated group showed better cell viability than the other groups. Control and alkali-heat-treated groups showed similar cell viabilities. In the ALP activity test, every surface-treated group showed significantly higher values than the control group. Especially, alkali-heat-treated group had the highest ALP activity. Conclusion: The results suggest that titanium surface modification could show better surface properties and biological characteristics, especially in the alkali-heat-treated surface.
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