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In vitro anti-skin-aging effects of dried pomegranate concentrated powder

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Purpose : In this study, we intended to observe the anti-wrinkle and moisturizing effects of dried pomegranate juice concentration powder (PCP) using in vitro test. Materials and methods : Antioxidant effects of PCP were determined by free radical scavenging capacity (DPPH assay) and the cytotoxicity of PCP was examined in human keratinocyte (HaCaT) and human primary dermal fibroblast-neonatal (HDF) cells. To investigate the moisturizing effect of PCP, hyaluronan synthesis was examined in HaCaT cells. Activity of procollagen production were assessed in HDF cells and elastase inhibition properties of PCP were evaluated in cell free condition, to determine their anti-wrinkle effects. Metalloproteinase 1 (MMP-1) activity was also assessed following UVB irradiation, in the current in vitro experiment. Results : No PCP treatment related significant cytotoxic effects were demonstrated against to the both HDF and HaCaT cells. PCP showed favorable free radical scavenging activities in dose-dependent manner. In PCP-treated HaCaT cells, hyaluronan synthesis was non-significantly but markedly increased, and pro-collagen productions were significantly increased in HDF cells, at all three different concentrations (0.25, 0.75 and 1 mg/ml), and elastase inhibitory activities were observed by PCP treatment. A significant decrease in UVB-induced MMP-1 activity was also observed in 1 mg/ml PCP-treated HDF cells as compared to those of UVB-exposed cells. Conclusions : Taken together, these results suggest that PCP has favorable antioxidant, anti-wrinkle and moisturizing effects without meaningful cytotoxicity on HDF and HaCaT cell lines.

Abstract

Purpose : In this study, we intended to observe the anti-wrinkle and moisturizing effects of dried pomegranate juice concentration powder (PCP) using in vitro test. Materials and methods : Antioxidant effects of PCP were determined by free radical scavenging capacity (DPPH assay) and the cytotoxicity of PCP was examined in human keratinocyte (HaCaT) and human primary dermal fibroblast-neonatal (HDF) cells. To investigate the moisturizing effect of PCP, hyaluronan synthesis was examined in HaCaT cells. Activity of procollagen production were assessed in HDF cells and elastase inhibition properties of PCP were evaluated in cell free condition, to determine their anti-wrinkle effects. Metalloproteinase 1 (MMP-1) activity was also assessed following UVB irradiation, in the current in vitro experiment. Results : No PCP treatment related significant cytotoxic effects were demonstrated against to the both HDF and HaCaT cells. PCP showed favorable free radical scavenging activities in dose-dependent manner. In PCP-treated HaCaT cells, hyaluronan synthesis was non-significantly but markedly increased, and pro-collagen productions were significantly increased in HDF cells, at all three different concentrations (0.25, 0.75 and 1 mg/ml), and elastase inhibitory activities were observed by PCP treatment. A significant decrease in UVB-induced MMP-1 activity was also observed in 1 mg/ml PCP-treated HDF cells as compared to those of UVB-exposed cells. Conclusions : Taken together, these results suggest that PCP has favorable antioxidant, anti-wrinkle and moisturizing effects without meaningful cytotoxicity on HDF and HaCaT cell lines.

참고문헌 (73)

  1. Gilchrest BA, "Skin aging and photoaging", Dermatology nursing, 2(2), pp.79~82 (1990)
  2. Cevenini E, "Human models of aging and longevity", Expert opinion on biological therapy, 8(9), pp.1393~1405 (2008)[DOI]
  3. Jurkiewicz BA, "Ultraviolet light-induced free radical formation in skin: an electron paramagnetic resonance study", Photochemistry and photobiology, 59(1), pp.1~4 (1994)[DOI]
  4. Messarah M, "Influence of thyroid dysfunction on liver lipid peroxidation and antioxidant status in experimental rats", Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie, 62(3), pp.301~310 (2010)[DOI]
  5. Rodriguez KJ, "A purified feverfew extract protects from oxidative damage by inducing DNA repair in skin cells via a PI3-kinase-dependent Nrf2", ARE pathway, 2013, pp.72(3), 304~310
  6. Campanini MZ, "Efficacy of topical formulations containing Pimenta pseudocaryophyllus extract against UVB-induced oxidative stress and inflammation in hairless mice", Journal of photochemistry and photobiology B, Biology, 127, pp.153~160 (2013)[DOI]
  7. Kim SR, "Anti-wrinkle and anti-inflammatory effects of active garlic components and the inhibition of MMPs via NF-kB signaling", PloS one, 8(9), pp.e73877~ (2013)[DOI]
  8. Oh J, "Radical scavenging activity-based and AP-1-targeted anti-inflammatory effects of lutein in macrophage-like and skin keratinocytic cells", Mediators of inflammation, 2013, pp.787042~ (2013)
  9. Poswig A, "Adaptive antioxidant response of manganese-superoxide dismutase following repetitive UVA irradiation", The Journal of investigative dermatology, 112(1), pp.13~18 (1999)[DOI]
  10. Fuchs J, "Modulation of UV-light-induced skin inflammation by D-alpha-tocopherol and L-ascorbic acid: a clinical study using solar simulated radiation", Free radical biology & medicine, 25(9), pp.1006~1012 (1998)[DOI]
  11. Kim YH, "Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient", Bioscience, biotechnology, and biochemistry, 72(2), pp.303~311 (2008)[DOI]
  12. Hunt G, "Eumelanin and phaeomelanin contents of human epidermis and cultured melanocytes", Pigment cell research, 8(4), pp.202~208 (1995)[DOI]
  13. Vincensi MR, "Phaeomelanin versus eumelanin as a chemical indicator of ultraviolet sensitivity in fair-skinned subjects at high risk for melanoma: a pilot study", Melanoma research, 8(1), pp.53~58 (1998)[DOI]
  14. Kvam E, "Pigmented melanocytes are protected against ultraviolet-A-induced membrane damage", The Journal of investigative dermatology, 121(3), pp.564~569 (2003)[DOI]
  15. Yamazaki F, "XPA gene-deficient, SCF-transgenic mice with epidermal melanin are resistant to UV-induced carcinogenesis", The Journal of investigative dermatology, 123(1), pp.220~228 (2004)[DOI]
  16. Wagner JK, "Skin responses to ultraviolet radiation: effects of constitutive pigmentation, sex, and ancestry", Pigment cell research, 15(5), pp.385~390 (2002)[DOI]
  17. Ortonne JP, "Photoprotective properties of skin melanin", The British journal of dermatology, 146(Suppl 61), pp.7~10 (2002)
  18. Yamaguchi Y, "Human skin responses to UV radiation: pigment in the upper epidermis protects against DNA damage in the lower epidermis and facilitates apoptosis", FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(9), pp.1486~1488 (2006)[DOI]
  19. Tada M, "Scavenging or quenching effect of melanin on superoxide anion and singlet oxygen", Journal of clinical biochemistry and nutrition, 46(3), pp.224~228 (2010)[DOI]
  20. Ito S, "Chemistry of mixed melanogenesis--pivotal roles of dopaquinone", Photochemistry and photobiology, 84(3), pp.582~592 (2008)[DOI]
  21. Khazaeli P, "An evaluation of extracts of five traditional medicinal plants from Iran on the inhibition of mushroom tyrosinase activity and scavenging of free radicals", International journal of cosmetic science, 31(5), pp.375~381 (2009)[DOI]
  22. Momtaz S, "Tyrosinase inhibition by extracts and constituents of Sideroxylon inerme L. stem bark, used in South Africa for skin lightening", Journal of ethnopharmacology, 119(3), pp.507~512 (2008)[DOI]
  23. Ng LT, "Potential antioxidants and tyrosinase inhibitors from synthetic polyphenolic deoxybenzoins", Bioorganic & medicinal chemistry, 17(13), pp.4360~4366 (2009)[DOI]
  24. Nugroho A, "Two new flavonol glycosides from Lamium amplexicaule L. and their in vitro free radical scavenging and tyrosinase inhibitory activities", Planta medica, 75(4), pp.364~366 (2009)[DOI]
  25. Wang KH, "Cosmetic applications of selected traditional Chinese herbal medicines", Journal of ethnopharmacology, 106(3), pp.353~359 (2006)[DOI]
  26. Solomon EI, "Oxygen Binding, Activation, and Reduction to Water by Copper Proteins", Angewandte Chemie (International ed in English), 40(24), pp.4570~4590 (2001)[DOI]
  27. Chen P, "<TEX>$O_2$<", TEX> activation by binuclear Cu sites: noncoupled versus exchange coupled reaction mechanisms, 2004, pp.101(36), 13105~13110
  28. Inoue T, "Quantum chemical approach to the mechanism for the biological conversion of tyrosine to dopaquinone", Journal of the American Chemical Society, 130(50), pp.16890~16897 (2008)[DOI]
  29. Rob D, "Copper Proteins and Copper Enzymes", pp.207~240 (1984), CRC Press
  30. 조은아, "생약재 추출물로부터 주름 개선 소재의 발굴 및 이를 이용한 화장품의 안정성 시험", 한국약용작물학회지, 19(2), pp.126~135 (2011), 한국약용작물학회
  31. 곽윤진, "각종 약용 식물로부터 피부노화 억제 관련 elastase 저해물질의탐색 및 추출조건", 한국약용작물학회지, 13(6), pp.213~216 (2005), 한국약용작물학회
  32. Wiedow O, "Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence", The Journal of biological chemistry, 265(25), pp.14791~14795 (1990)
  33. Gil MI, "Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing", Journal of agricultural and food chemistry, 48(10), pp.4581~4589 (2000)[DOI]
  34. Noda Y, "Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin", Journal of agricultural and food chemistry, 50(1), pp.166~171 (2002)[DOI]
  35. Singh RP, "Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models", Journal of agricultural and food chemistry, 50(1), pp.81~86 (2002)[DOI]
  36. Kim ND, "Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer", Breast cancer research and treatment, 71(3), pp.203~217 (2002)[DOI]
  37. Maity N, "Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity", Journal of ethnopharmacology, 137(3), pp.1300~1305 (2011)[DOI]
  38. Chan YY, "Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells", Journal of ethnopharmacology, 137(3), pp.1183~1188 (2011)[DOI]
  39. Imokawa G, "Recent advances in characterizing biological mechanisms underlying UV-induced wrinkles: a pivotal role of fibrobrast-derived elastase", Arch dermatol Res, 300(1), pp.7~20 (2008)[DOI]
  40. 김은주, "Skin Aging and Photoaging Alter Fatty Acids Composition, Including 11,14,17-eicosatrienoic Acid, in the Epidermis of Human Skin", Journal of Korean Medical Science, 25(6), pp.980~983 (2010), 대한의학회
  41. Kang SJ, "Inhibitory effects of pomegranate concentrated solution on the activities of hyaluronidase, tyrosinase, and metalloproteinase", Journal of cosmetic science, 66(3), pp.145~159 (2015)
  42. 강수진, "석류농축액의 광노화에 의한 주름 개선 효과", 대한예방한의학회지, 19(1), pp.109~116 (2015), 대한예방한의학회
  43. Kang SJ, "Inhibitory effect of dried pomegranate concentration powder on melanogenesis in B16F10 melanoma cells; involvement of p38 and PKA signaling pathways", International journal of molecular sciences, 16(10), pp.24219~24242 (2015)[DOI]
  44. Kang SJ, "Beneficial effects of dried pomegranate juice concentrated powder on ultraviolet B-induced skin photoaging in hairless mice", Experimental and therapeutic medicine, 14(2), pp.1023~1036 (2017)[DOI]
  45. Jin-Ryul Hu, "Skin tissue homogenate analysis for ceramide and TGF-β1 contents with TGF-β1 mRNA expressions after treatment of pomegranate concentrated solution and dried pomegranate concentrate powder in mice", 대한한의학회지, 37(4), pp.1~9 (2016), 대한한의학회
  46. 강수진, "Evaluation of the in vivo skin moisturizing effects and underlying mechanisms of pomegranate concentrate solution and dried pomegranate concentrate powder", 대한한의학회지, 37(2), pp.12~22 (2016), 대한한의학회
  47. Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181(4617), pp.1199~ (1958)[DOI]
  48. Jiang P, "Effective targeting of the tumor microenvironment for cancer therapy", Anticancer research, 32(4), pp.1203~1212 (2012)
  49. 허진철, "천마추출물의 항산화 및 항암 활성", Food Science and Preservation, 13(1), pp.83~87 (2006), 한국식품저장유통학회
  50. Losso JN, "Inhibition of matrix metalloproteinase-1 activity by the soybean Bowman-Birk inhibitor", Biotechnology letters, 26(11), pp.901~905 (2004)[DOI]
  51. Chen AY, "Entacapone is an Antioxidant More Potent than Vitamin C and Vitamin E for Scavenging of Hypochlorous Acid and Peroxynitrite, and the Inhibition of Oxidative Stress-Induced Cell Death", Medical science monitor : international medical journal of experimental and clinical research, 22, pp.687~696 (2016)
  52. Lijnen P, "Transforming growth factor-beta 1-induced collagen production in cultures of cardiac fibroblasts is the result of the appearance of myofibroblasts", Methods and findings in experimental and clinical pharmacology, 24(6), pp.333~344 (2002)[DOI]
  53. Morihara K, "Phosphoramidon as an inhibitor of elastase from Pseudomonas aeruginosa", The Japanese journal of experimental medicine, 48(1), pp.81~84 (1978)
  54. 김동욱, "Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates", 한국축산식품학회지, 34(6), pp.844~851 (2014), 한국축산식품학회
  55. Kikuchi K, "Oral N-acetylglucosamine supplementation improves skin conditions of female volunteers: Clinical evaluation by a microscopic three-dimensional skin surface analyzer", Journal of applied cosmetology, 20(2), pp.143~152 (2002)
  56. Uitterlinden EJ, "Glucosamine increases hyaluronic acid production in human osteoarthritic synovium explants", BMC musculoskeletal disorders, 9, pp.120~ (2008)[DOI]
  57. Lateef H, "All-transretinoic acid suppresses matrix metalloproteinase activity and increases collagen synthesis in diabetic human skin in organ culture", The American journal of pathology, 165(1), pp.167~174 (2004)[DOI]
  58. Jurzak M, "Influence of retinoids on skin fibroblasts metabolism in vitro", Acta poloniae pharmaceutica, 65(1), pp.85~91 (2008)
  59. Afaq F, "Anthocyanin- and hydrolyzable tannin-rich pomegranate fruit extract modulates MAPK and NF-kB pathways and inhibits skin tumorigenesis in CD-1 mice", International journal of cancer, 113(3), pp.423~433 (2005)[DOI]
  60. Wang RF, "Bioactive compounds from the seeds of Punica granatum (pomegranate)", Journal of natural products, 67(12), pp.2096~2098 (2004)[DOI]
  61. Yoshimura M, "Inhibitory effect of an ellagic acid-rich pomegranate extract on tyrosinase activity and ultraviolet-induced pigmentation", Bioscience, biotechnology, and biochemistry, 69(12), pp.2368~2373 (2005)[DOI]
  62. Ross JA, "Dietary flavonoids: bioavailability, metabolic effects, and safety", Annual review of nutrition, 22, pp.19~34 (2002)[DOI]
  63. Soobrattee MA, "Chemopreventive actions of polyphenolic compounds in cancer", BioFactors (Oxford, England), 27(1-4), pp.19~35 (2006)[DOI]
  64. Maas JL, "Ellagic acid, an anticarcinogen in fruits, especially in strawberries: a review", HortScience, 26(1), pp.10~14 (1991)
  65. Thresiamma KC, "Inhibition of liver fibrosis by ellagic acid", Indian journal of physiology and pharmacology, 40(4), pp.363~366 (1996)
  66. Osawa T, "Inhibition of in vitro lipid peroxidation by ellagic acid", Journal of agricultural and food chemistry, 35(5), pp.808~812 (1987)[DOI]
  67. Shimogaki H, "In vitro and in vivo evaluation of ellagic acid on melanogenesis inhibition", International journal of cosmetic science, 22(4), pp.291~303 (2000)[DOI]
  68. Yamane T, "Hydrocellular foam dressing promotes wound healing along with increases in hyaluronan synthase 3 and PPARa gene expression in epidermis", PloS one, 8(8), pp.e73988~ (2013)[DOI]
  69. Frenkel JS, "The role of hyaluronan in wound healing", International wound journal, 11(2), pp.159~163 (2014)[DOI]
  70. Liao PL, "Anti-ageing effects of alpha-naphthoflavone on normal and UVB-irradiated human skin fibroblasts", Experimental dermatology, 21(7), pp.546~548 (2012)[DOI]
  71. Hyeong-Dong Kim, "Effects of β-Glucan from Aureobasidium pullulans on Acute Inflammation in Mice", Archives of Pharmacal Research, 30(3), pp.323~328 (2007), 대한약학회
  72. Adams LS, "Pomegranate juice, total pomegranate ellagitannins, and punicalagin suppress inflammatory cell signaling in colon cancer cells", Journal of agricultural and food chemistry, 54(3), pp.980~985 (2006)[DOI]
  73. Orgil O, "The antioxidative and anti-proliferative potential of non-edible organs of the pomegranate fruit and tree", LWT-Food Science and Technology, 58(2), pp.571~577 (2014)[DOI]

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