Inhibitory Effects of a Combination of Grapefruit and Rosemary Extracts for Alleviating UV-Induced Skin Ageing
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초록
Ultraviolet (UV) radiation is associated with the development of extrinsic skin aging. We performed in vivo assays in order to investigate the protective effect of a combination of grapefruit and rosemary extracts (cG&Re) on UVB-induced skin aging. The results indicated that cG&Re displayed elastase inhibitory activity in a dose-dependent manner. Topical application of cG&Re mitigated photo-aging related lesions such as skin erythema and thickening in photo-aged BALB/c mice dorsal skin, by preventing UVB-induced collagen degradation. Immunohistochemical analyses revealed that cG&Re stimulated SIRT-1 expression, and suppressed MMP-1 and IL-1β expression. It was observed that expression of MMP-1 and -13 mRNA was downregulated in the cG&Re-treated group. Furthermore, cG&Re treatment drastically suppressed protein expression of MMP-1 and regulated the phosphorylation of p-38 kinase. As expected, oral administration of cG&Re resulted in the same SIRT-1, MMP-1, and IL-1β expression patterns observed upon topical application of cG&Re in the UV-induced mice model. Overall, the current results demonstrated that cG&Re attenuated both the downregulation of MMP-1 expression and up-regulation of SIRT-1 expression, as well as decreased phosphorylation of MAPK in UVB-induced skin ageing mice model, suggesting that cG&Re might be used as an internal food ingredient for beauty-purposes as well as a functional food material.
Abstract
Ultraviolet (UV) radiation is associated with the development of extrinsic skin aging. We performed in vivo assays in order to investigate the protective effect of a combination of grapefruit and rosemary extracts (cG&Re) on UVB-induced skin aging. The results indicated that cG&Re displayed elastase inhibitory activity in a dose-dependent manner. Topical application of cG&Re mitigated photo-aging related lesions such as skin erythema and thickening in photo-aged BALB/c mice dorsal skin, by preventing UVB-induced collagen degradation. Immunohistochemical analyses revealed that cG&Re stimulated SIRT-1 expression, and suppressed MMP-1 and IL-1β expression. It was observed that expression of MMP-1 and -13 mRNA was downregulated in the cG&Re-treated group. Furthermore, cG&Re treatment drastically suppressed protein expression of MMP-1 and regulated the phosphorylation of p-38 kinase. As expected, oral administration of cG&Re resulted in the same SIRT-1, MMP-1, and IL-1β expression patterns observed upon topical application of cG&Re in the UV-induced mice model. Overall, the current results demonstrated that cG&Re attenuated both the downregulation of MMP-1 expression and up-regulation of SIRT-1 expression, as well as decreased phosphorylation of MAPK in UVB-induced skin ageing mice model, suggesting that cG&Re might be used as an internal food ingredient for beauty-purposes as well as a functional food material.
참고문헌 (43)
- Bissett, D., "Photoprotective effect of superoxide-scavenging antioxidants against ultraviolet radiation-induced chronic skin damage in the hairless mouse", Photodermatol Photo, 7(2), pp.56~62 (1990)
- Cao, C., "SIRT1 confers protection against UVB‐and H2O2‐induced cell death via modulation of p53 and JNK in cultured skin keratinocytes", J Cell Mol Med, 13(9b), pp.3632~3643 (2009)[DOI]
- Chanda, S., "Combination therapy:Synergism between natural plant extracts and antibiotics against infectious diseases, Microbiol Book Series", pp.520~529 (2011)
- Cheung, S., "Anti-proliferative and antioxidant properties of rosemary Rosmarinus officinalis", Oncol Rep., 17(6), pp.1525~1531 (2007)
- Choi, S., "Apigenin inhibits UVA-induced cytotoxicity in vitro and prevents signs of skin aging in vivo", Int J Mol Med, 38(2), pp.627~634 (2016)
- Cristóbal-Luna, J. M., "Grapefruit and its biomedical, antigenotoxic and chemopreventive properties", Food Chem Toxicol, 112, pp.224~234 (2018)
- Cuvelier, M. E., "Antioxidative activity and phenolic composition of pilot‐plant and commercial extracts of sage and rosemary", J Am Oil Chem Soc, 73(5), pp.645~652 (1996)
- D'Orazio, J., "UV radiation and the skin", Int J Mol Sci, 14(6), pp.12222~12248 (2013)
- Erenmemisoglu, A., "Effect of a Rosmarinus officinalis leave extract on plasma glucose levels in normoglycaemic and diabetic mice", Die Pharmazie, 52(8), pp.645~ (1997)
- Fisher, G. J., "c-Jun–dependent inhibition of cutaneous procollagen transcription following ultraviolet irradiation is reversed by all-trans retinoic acid", J Clin Invest, 106(5), pp.663~670 (2000)
- Fisher, G. J., "Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin", Am J Pathol, 174(1), pp.101~114 (2009)
- Fisher, G. J., "Pathophysiology of premature skin aging induced by ultraviolet light", New Engl J Med, 337(20), pp.1419~1429 (1997)
- García‐Martínez, E., "Antioxidant and anti‐inflammatory activities of freeze‐dried grapefruit phenolics as affected by gum arabic and bamboo fibre addition and microwave pretreatment", J Sci Food Agr, 98(8), pp.3076~3083 (2018)
- Haigis, M. C., "Handbook of the biology of aging", pp.243~274 (2011), Elsevier
- Hubbard, B. P., "Small molecule SIRT1activators for the treatment of aging and age-related diseases", Trends Pharmacol Sci, 35(3), pp.146~154 (2014)
- Inomata, S., "Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse", J Invest Dermatol, 120(1), pp.128~134 (2003)
- Kang, M., "Oral intake of collagen peptide attenuates ultraviolet B irradiation-induced skin dehydration in vivo by regulating hyaluronic acid synthesis", Int J Mol Sci, 19(11), pp.3551~ (2018)
- Kaurinovic, B., "Antioxidants" (2019), Intech-Open
- Khan, I. A., "Leung's Encyclopedia of Common Natural Ingredients: used in food, drugs and cosmetics" (2011), John Wiley & Sons
- Kim, J. -K., "Gingerol prevents UVB-induced ROS production and COX-2 expression in vitro and in vivo", Free Radical Research, 41(5), pp.603~614 (2007)
- 구현정, "Inhibitory Effects of Ethanol Extract from Angelica tenuissima Root on Oxidative Stress and Melanogenesis", 한국자원식물학회지, 31(4), pp.312~321 (2018), 한국자원식물학회[DOI]
- Kraunsoe, J. A., "Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor", Biochemistry, 35(28), pp.9090~9096 (1996)
- Kruger, N. J, "The Protein Protocols Handbook", pp.17~24 (2009), Springer
- Liu, Y., "Protective effects of Euphrasia officinalis extract against ultraviolet B-induced photoaging in normal human dermal fibroblasts", Int J Mol Sci, 19(11), pp.3327~ (2018)
- McCullough, J. L., "Prevention and treatment of skin aging", Ann Ny Acad Sci, 1067(1), pp.323~331 (2006)
- Orjalo, A. V., "Cell surface-bound IL-1α is an upstream regulator of the senescence-associated IL-6", IL-8 cytokine network, 2009, pp.106(40), 17031~17036
- Park, J. E., "The protective effect of K aempferia parviflora extract on UVB‐induced skin photoaging in hairless mice", Photodermatol Photo, 30(5), pp.237~245 (2014)
- Pérez-Sánchez, A., "Protective effects of citrus and rosemary extracts on UVinduced damage in skin cell model and human volunteers", J Photochem Photobiol B, 136, pp.12~18 (2014)
- Quan, T., "Matrix-degrading metalloproteinases in photoaging", J Invest Derm Symp p (2009), Elsevier
- Rittié, L., "UV-light-induced signal cascades and skin aging", Ageing Res Rev, 1(4), pp.705~720 (2002)
- Rittié, L., "Natural and sun-induced aging of human skin", Csh Perspect Med, 5(1), pp.a015370~ (2015)
- Rosenthal, F. S., "The effect of prescription eyewear on ocular exposure to ultraviolet radiation", Am J Public Health, 76(10), pp.1216~1220 (1986)
- Sekhon-Loodu, S., "Evaluation of antioxidant, antidiabetic and antiobesity potential of selected traditional medicinal plants", Frontiers in Nutrition, 6, pp.53~ (2019)
- Son, Y., "Reactive oxygen species in the activation of MAP kinases", Method Enzymol, 528, pp.27~48 (2013), Elsevier
- Sun, Y., "Signaling pathway of MAPK", ERK in cell proliferation, differentiation, migration, senescence and apoptosis, 2015, pp.35(6), 600~604
- Varani, J., "Vitamin a antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin1", J Invest Dermatol, 114(3), pp.480~486 (2000)
- Vincenti, M. P., "Transcriptional regulation of collagenase(MMP-1, MMP-13)genes in arthritis : integration of complex signaling pathways for the recruitment of gene-specific transcription factors", Arthritis Res Ther, 4(3), pp.157~ (2002)
- Vogt, T. M., "RNA fingerprinting displays UVB-specific disruption of transcriptional control in human melanocytes", Cancer Res, 57(16), pp.3554~3561 (1997)
- Wang, X. -y., "UVB-irradiated human keratinocytes and interleukin-1α indirectly increase MAP kinase", AP-1 activation and MMP-1 production in UVA-irradiated dermal fibroblasts, 2006, pp.119(10), 827~831
- Wen, W., "Astragaloside exerts anti-photoaging effects in UVB-induced premature senescence of rat dermal fibroblasts through enhanced autophagy", Arch Biochem Biophys, 657, pp.31~40 (2018)
- Xie, M., "A pivotal role for endogenous TGF-β-activated kinase-1 in the LKB1", AMP-activated protein kinase energy-sensor pathway, 2006, pp.103(46), 17378~17383
- Guang-Hua Xu, "Free Radical Scavenging and Antielastase Activities of Flavonoids from the Fruits of Thuja orientalis", Archives of Pharmacal Research, 32(2), pp.275~282 (2009), 대한약학회
- Zhou, X., "Synergistic effects of Chinese herbal medicine : a comprehensive review of methodology and current research", Front Pharmacol, 7, pp.201~ (2016)
