호두 껍데기 에탄올 추출물의 항산화 및 각질형성세포에서 Tumor Necrosis Factor Alpha에 의해 유도된 Metalloproteinase-1 발현 저해 효과
Walnut Husk Ethanol Extract Possess Antioxidant Activity and Inhibitory Effect of Matrix Metalloproteinase-1 Expression Induced by Tumor Necrosis Factor Alpha in Human Keratinocyte
1
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
This study was evaluated antioxidant activity of ethanol extracts of Juglans regia (walnut) husk (WHE) using 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Also, cell viability of WHE on human keratinocyte (HaCaT) was evaluated by sulforhodamine B (SRB) assay. Inhibitory effect of WHE on tumor necrosis factor alpha (TNF-α)induced Matrix Metalloproteinase-1 (MMP-1) expression in HaCaT was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). As the results, WHE was showed similar antioxidant activity to vitamin C at concentration of 6.25, 12.5, 25, 50 μg/mL. WHE of low concentration (6.25, 12.5 and 25 μg/mL) didn’t exhibited cytotoxicity in HaCaT. However, treatment of 50 and 100 μg/mL WHE were showed cell viability of about 98.4% and 91.1%, respectively, in same cells. Matrix Metalloproteinase-1 (MMP-1) mRNA were induced by tumor necrosis factor alpha (TNF-α) in HaCaT cells and WHE treatment of 10, 20, 50 and 100 μg/mL were decreased MMP-1 mRNA with dose-dependent manner. Exhibiting excellent antioxidant activity with high cell viability, and potent inhibitory effect on MMP-1 mRNA, ethanol extracts of walnut husk extracts can be applied in cosmetics as a MMP-1 inhibitor.
Abstract
This study was evaluated antioxidant activity of ethanol extracts of Juglans regia (walnut) husk (WHE) using 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Also, cell viability of WHE on human keratinocyte (HaCaT) was evaluated by sulforhodamine B (SRB) assay. Inhibitory effect of WHE on tumor necrosis factor alpha (TNF-α)induced Matrix Metalloproteinase-1 (MMP-1) expression in HaCaT was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). As the results, WHE was showed similar antioxidant activity to vitamin C at concentration of 6.25, 12.5, 25, 50 μg/mL. WHE of low concentration (6.25, 12.5 and 25 μg/mL) didn’t exhibited cytotoxicity in HaCaT. However, treatment of 50 and 100 μg/mL WHE were showed cell viability of about 98.4% and 91.1%, respectively, in same cells. Matrix Metalloproteinase-1 (MMP-1) mRNA were induced by tumor necrosis factor alpha (TNF-α) in HaCaT cells and WHE treatment of 10, 20, 50 and 100 μg/mL were decreased MMP-1 mRNA with dose-dependent manner. Exhibiting excellent antioxidant activity with high cell viability, and potent inhibitory effect on MMP-1 mRNA, ethanol extracts of walnut husk extracts can be applied in cosmetics as a MMP-1 inhibitor.
참고문헌 (20)
- Ahmad H, "Antiglycation and antioxidation properties of Juglans regia and Calendula officinalis: possible role in reducing diabetic complications and slowing down ageing", J. Tradit. Chin. Med, 32, pp.411~414 (2012)
- Akbari V, "Antiradica l activity of different parts of Walnut (Juglans regia L.) fruit as a function of genotype", Food Chem, 135, pp.2404~2410 (2012)
- An BJ, "Inhibition of enzyme activities and the antiwrinkle effect of polyphenol isolated from the persimmon leaf (Diospyros kaki folium) on human skin", Dermatol. Surg, 31, pp.848~854 (2005)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Chaieb K, "Antioxidant properties of the essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species", Mycoses, 50, pp.403~406 (2007)
- Choi HJ, "In vitro antienterovirus 71 activity of gallic acid from Woodfordia fruticosa flowers", Lett. Appl. Microbiol, 50, pp.438~440 (2010)
- Clark AM, "Antimicrobial activity of juglone", Phytother. Res, 4, pp.11~14 (1990)
- Comstock SS, "Effect of walnut (Juglans regia ) polyphenolic compounds on ovalbumin-specific IgE induction in female BALB", c mice, 2010, pp.1190, 58~69
- Darout IA, "entification and quantification of some potentially antimicrobial anionic components in miswak extract", Ind. J. Pharmacol, 32, pp.11~14 (2000)
- Hajlaoui H, "Comparison of chemical composition and antimicrobial activities of Mentha longifolia L. ssp. longifolia essential oil from two Tunisian localities (Gabes and Sidi Bouzid)", Ann. Microbiol, 58, pp.513~520 (2008)
- Cheolmin Kim, "Low-dose UVB irradiation stimulates matrix metalloproteinase-1 expression via a BLT2-linked pathway in HaCaT cells", Experimental and Molecular Medicine, 42(12), pp.833~841 (2010), 생화학분자생물학회
- Kim HS, "Inhibition of UVBinduced wrinkle formation and MMP-9 expression by mangiferin isolated from Anemarrhena asphodeloides", Eur. J. Pharmacol, 689, pp.38~44 (2012)
- Maqsood S, "Comparative studies of four different phenolic compounds on in vitro antioxidative activity and the preventive effect on lipid oxidation of fish oil emulsion and fish mince", Food Chem, 119, pp.123~132 (2010)
- 박주아, "소리쟁이(Rumex crispus L.) 잎 추출물의 항산화 효과 및 각질세포 보호효과", 한국인체미용예술학회지, 12(2), pp.189~198 (2011), 한국인체미용예술학회
- Quan T, "Matrix-degrading metalloproteinases in photoaging", J. Investig. Dermatol. Symp. Proc, 14, pp.20~24 (2009)
- 유민정, "상백피 추출물의 피부상재균에 대한 항균활성과 항산화 효과", 한국인체미용예술학회지, 11(2), pp.87~98 (2010), 한국인체미용예술학회
- 유민정, "질경이 추출물의 항산화 효과 및 각질세포 보호효과", 한국인체미용예술학회지, 11(3), pp.15~25 (2010), 한국인체미용예술학회
- Runyoro DK, "Screening of Tanzanian medicinal plants for anti-Candida activity", BMC Complement Altern. Med, 6, pp.1~10 (2006)
- Stampar F, "Traditional walnut liqueur–Cocktail of phenolics", Food Chem, 95, pp.627~631 (2006)
- Yuan H, "Synthesis of derivatives of methyl rosmarinate and their inhibitory activities against matrix metalloproteinase-1 (MMP-1)", Eur. J. Med. Chem, 62, pp.1748~2157 (2013)