세균 Pseudomonas sp. YK-32 균주에 의한 Formaldehyde 분해 최적조건
Optimum Conditions of Formaldehyde Degradation by the Bacterium Pseudomonas sp. YK-32
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Formaldehyde, an indoor volatile organic compound, is considered toxic due to its carcinogenic risk. Recently, we isolated a formaldehyde-degrading bacterium Pseudomonas sp. YK-32. A crude enzyme prepared from YK-32 also degraded formaldehyde, suggesting that YK-32 cells have formaldehyde hydrogenase activity which is one of the important factors in formaldehyde degradation. The formaldehyde hydrogenase activity was increased 1.25 fold by adding 0.1% glucose and formaldehyde to the culture medium. In addition, treatment with 1mM EDTA as a permeabilizer promoted the degradation of formaldehyde and increased the enzymatic activity.
Abstract
Formaldehyde, an indoor volatile organic compound, is considered toxic due to its carcinogenic risk. Recently, we isolated a formaldehyde-degrading bacterium Pseudomonas sp. YK-32. A crude enzyme prepared from YK-32 also degraded formaldehyde, suggesting that YK-32 cells have formaldehyde hydrogenase activity which is one of the important factors in formaldehyde degradation. The formaldehyde hydrogenase activity was increased 1.25 fold by adding 0.1% glucose and formaldehyde to the culture medium. In addition, treatment with 1mM EDTA as a permeabilizer promoted the degradation of formaldehyde and increased the enzymatic activity.
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