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하천 저질에서 분리한 Formaldehyde 분해 미생물의 특징

Characterization of Formaldehyde-degrading Bacteria Isolated from River Sediment

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주제분류수산학

초록

A bacterium growing on formaldehyde as a sole carbon source was isolated by the dilution method from an enrichment culture containing formaldehyde. The isolated strain, YK-32, was identified as Pseudomonas sp. by morphological, biochemical, and genetic analyses. Pseudomonas sp. YK-32 completely degraded 0.05% formaldehyde within 24hrs. The isolated strain had a high level of formaldehyde dehydrogenase activity, which is thought to be one of the important factors for formaldehyde degradation, when cells were cultivated in the presence of formaldehyde.

Abstract

A bacterium growing on formaldehyde as a sole carbon source was isolated by the dilution method from an enrichment culture containing formaldehyde. The isolated strain, YK-32, was identified as Pseudomonas sp. by morphological, biochemical, and genetic analyses. Pseudomonas sp. YK-32 completely degraded 0.05% formaldehyde within 24hrs. The isolated strain had a high level of formaldehyde dehydrogenase activity, which is thought to be one of the important factors for formaldehyde degradation, when cells were cultivated in the presence of formaldehyde.

참고문헌 (16)

  1. Berns, K.I, "Isolation of the high molecular DNA from Haemophilus influenzae", J. Mol. Biol, 11, pp.476~490 (1965)
  2. Bradford, M.M, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
  3. Choi, J.H., "Cloning and characterization of a gene cluster for cyclohexanoneol oxidation in Rhodococcus sp", TK6. J. Microbiol. Biotechnol, 16, pp.511~518 (2006)
  4. Dorsev, C.W, "Analysis of pVU3695, a plasmid encoding glutathione-dependent formalde- hyde dehydrogenase activity and formaldehyde resistance in the Escherichia coli VU3695 clinical strain", Plasmid, 51, pp.116~126 (2003)
  5. Dunbar, J., "Assess- ment of microbial diversity in four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis", Appl. Environ. Micro- biol, 66, pp.2943~2950 (2000)
  6. Echenique, J.R., "Acinetobacter baumannii has two genes encoding glutathione-dependent formaldehyde dehydrogenase:evidence for differential regulation in response to iron.Micro-biology,147,2805-2815", Micro- biology, 147, pp.2805~2815 (2001)
  7. Ito, K., "Cloning and high-level expression of the glutathione- independent formaldehyde dehydrogenase gene from Pseudomonas putida", J. Bacteriol, 176, pp.2983~2941 (1994)
  8. Karlsson, E, "Influence of desorption on the indoor concentration of toxic gases", J. Hazard. Mater, 49, pp.15~27 (1996)
  9. Kaulfers, P.M, "Demonstration of formaldehyde dehydrogenase activity in formalde- hyde-resistant Enterobacteriaceae", FEMS Microbiol. Lett, 63, pp.335~338 (1991)
  10. Kim, Y.M., "Glutathione-dependent biotrans- formation of the fungicide chlorothalonil", J. Agric. Food Chem, 52, pp.4192~4196 (2004)
  11. Kim, Y.M., "Cloning and characterization of a catechol-degrading gene cluster from 3,4-dichloro- aniline degrading bacterium Pseudomonas sp.", KB35B.J. Agric. Food Chem, 55, pp.4722~4727 (2007)
  12. Lee, S.I, "A study on airborne concentration of formaldehyde in underground environments in Taegu city", J. Kor. Soc. Environ. Admin, 3, pp.49~66 (1997)
  13. Na, K., "Cloning and phylogenetic analysis of two diferent bphC genes and bphD gene from PCB-degrading bacterium, Pseudomonas sp", strain SY5. J. Microbiol. Bio- technol, 11, pp.668~676 (2001)
  14. Ryu, B.H., "Continuous degradation of formaldehyde by Pseudo- monas putida H-5 using rotating disc contactor", Kor. J. Biotechnol. Bioeng, 8, pp.42~48 (1993)
  15. Schoenberg, J.B, "Airway disease caused by phenolics (phenol-formaldehyde) resin exposure, Arch", Environ. Health, 30, pp.572~577 (1975)
  16. Shim, S.H, "Characterization and assessment of indoor air quality in newly constructed apartments-volatile organic compounds and formalde- hyde", Kor. J. Environ. Health, 32, pp.275~281 (2006)

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