산림 토착 미생물 군집에 미치는 유류 첨가제 노출 농도 및 시간의 영향
Effect of Exposure Concentration and Time of Fuel Additives on Indigenous Microbial Community in Forest
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The toxicity effects of methyl tert-butyl ether (MTBE), tert-butyl alcohol (TBA) and formaldehyde (FA) on indigenous microbial community in forest soil were studied. MTBE, TBA and FA with different concentrations were added into microcosms containing forest soil samples. After 10 and 30 days, total viable cell number and dehydrogenase activity in the microcosms were evaluated. Bacterial communities in the microcosms were also analyzed using a denaturing gradient gel electrophoresis (DGGE). Dehydrogenase activity and total viable cell number were decreased according to the increase of MTBE, TBA and FA concentrations (P<0.05). FA toxicity was the the highest, and TBA toxicity was the lowest. The results of principal component analysis using DGGE fingerprints showed that the microbial communities contaminated with MTBE, TBA and FA were grouped by exposure time, but not by exposure concentration. Dominant species in the microcosms were as follows: gamma-proteobacteria, Chloroflexi, Actinobacteria, Clostridia and Bacilli f or M TBE, TBA a nd FA.
Abstract
The toxicity effects of methyl tert-butyl ether (MTBE), tert-butyl alcohol (TBA) and formaldehyde (FA) on indigenous microbial community in forest soil were studied. MTBE, TBA and FA with different concentrations were added into microcosms containing forest soil samples. After 10 and 30 days, total viable cell number and dehydrogenase activity in the microcosms were evaluated. Bacterial communities in the microcosms were also analyzed using a denaturing gradient gel electrophoresis (DGGE). Dehydrogenase activity and total viable cell number were decreased according to the increase of MTBE, TBA and FA concentrations (P<0.05). FA toxicity was the the highest, and TBA toxicity was the lowest. The results of principal component analysis using DGGE fingerprints showed that the microbial communities contaminated with MTBE, TBA and FA were grouped by exposure time, but not by exposure concentration. Dominant species in the microcosms were as follows: gamma-proteobacteria, Chloroflexi, Actinobacteria, Clostridia and Bacilli f or M TBE, TBA a nd FA.
참고문헌 (25)
- An, Y. J., "Assessing soil ecotoxicity of Methyl tertbutyl ether using earthworm bioassay; closed soil microcosm test for volatile organic compounds", Environmental Pollution, 134, pp.181~186 (2005)
- 변상혁, "국내 하천들의 수질, 하천 퇴적물, 주변 토양 및 별늑대거미 체내 중금속 축적량을 통한 환경오염도 평가", 한국환경과학회지, 17(3), pp.287~296 (2008), 한국환경과학회
- Cho, K., "A study on transport characteristics of MTBE(Methyl tert-butyl ether) in soil. Environmental Engineering", Research, 13, pp.51~65 (2008)
- CHO, WONSIL, "Bacterial Communities of Biofilms Sampled from Seepage Groundwater Contaminated with Petroleum Oil", Journal of Microbiology and Biotechnology, 15(5), pp.952~964 (2005), 한국미생물·생명공학회
- Cho, W. S., "Effect of Methyl tertbutyl ether and its metabolites on the microbial activity and diversity in tidal mud sediment", Korea Journal of Microbiology and Biotechnology
- Davis, L. C., "A review of bioremediation and natural attenuation of MTBE", Environmental Process, 23, pp.243~252 (2004)
- Deeb, R. A., "Substrate interactions in BTEX and MTBE mixtures by an MTBE-degrading isolate", Environmental Science & Technology, 35, pp.312~317 (2001)
- Felske, A, "Spatial homogeneity of abundant bacterial 16S rRNA molecules in in glassland soils", Microbial Ecology, 36, pp.31~36 (1998)
- Feris, K. P., "A shallow BTEX and MTBE contaminated aquifer supports a diverse microbial community", Microbial Ecology, 48, pp.589~600 (2004)
- Germion, F. A, "Comparative 16S rDNA and 16s rRNA sequence analysis indicated tha Actinobacteria might be a dominant part of the metabolically active bacteria in heavy metalcontaminated bulk and rhizosphere soil", Environmental Microbiology, 10, pp.869~907 (2003)
- 홍선화, "유류 분해 근권세균 Rhodococcus sp. 412와 옥수수를 활용한 유류 오염 토양의 정화", 한국미생물·생명공학회지, 35(3), pp.150~157 (2007), 한국미생물·생명공학회
- Hyun, B. K., "Relationship Between Soil Water- Stable Aggregates and Physico-chemical Soil Properties", Korea Journal of Soil Science Society, 40, pp.57~63 (2007)
- Jung, S. S. Y., "(A) Study on adsorption and diffusion t ransport o f MTBE(Methyl t ertiary b utyl e ther) into the soil", 석사, pp.2004
- Kim, J. G., "A Survey and Some Heavy Metal Contents of Water and Rice in the Joenbuk Area of Korea", Korea Journal of Environmental Health, 32, pp.556~559 (2006)
- Kim, Y. A., "Comparison of Venting Modes for Bench Scale Treatment of Diesel Contaminated Soil", Korea Journal of Environmental Health, 32, pp.499~505 (2006)
- Kong, H. Y., "Physicochemical Characteristics and Microbial Activity in the Various Urban Soils)", Korea Journal of Ecology, 23, pp.269~375 (2000)
- Lee, H. M., "Crude oil Degradation and Activity of Microorganism Isolated from Soil", Journal of Environmental Research, 1, pp.129~138 (2001)
- Lin, K., "Metal toxicity to Chlorella pyrenoidosa assessed by a short-term continuous test", Journal of Hazardous Materials, 142, pp.236~241 (2007)
- Na, Y. E., "Toxicity o f Organic Waste- Contaminated Soil on Earthworm (Eisenia fetida)", Korean Society of Soil Science and Fertilizer, 40, pp.51~56 (2007)
- Perez-de-Mora, A., "Microbial community structure and function in a soil contaminated by heavy metals; effects of plant growth and different amendments", Soil Biology & Biochemistry, 38, pp.327~341 (2006)
- Savolainen, H., "Biochemical effects of MTBE in extended vapor exposure in rats", Archives of Toxicology, 57 (1995)
- Shen, G., "Interaction of polycyclic aromatic hydrocarbons and heavy metals on soil enzyme", Chemospere, 61, pp.1175~1182 (2005)
- Squillace, P. J., "Review of the environmental behavior and fate of MTBE", Environmental Toxicology and Chemistry, 16, pp.1836~1844 (1997)
- Yoon, B. J., "Evaluating the Impacts of Long-Term Use of Agricultural Chemicals on a Soil Ecosystem by Structural Analysis of Bacterial Community", Korea Journal of Microbiology and Biotechnology, 39, pp.260~266 (2003)
- 윤정기, "유류오염부지에서 자연저감기기법 적용 사례연구 Ⅱ. 지하수모니터링에 의한 저연저감 평가", 지하수토양환경, 9(3), pp.38~48 (2004), 한국지하수토양환경학회