다양한 토양 환경에서 Methyl tert-Butyl Ether와 그의 대사산물이 노출되었을 때 미생물 군집에 미치는 영향: 논, 밭, 갯벌 시료 비교
Effect of Methyl tert-butyl Ether and Its Metabolites on the Microbial Population: Comparison of Soil Samples from Rice Field, Leek Patch and Tidal Mud Flat
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Toxic effect of methyl tert-butyl ether (MTBE), tert-butyl alcohol (TBA) and formaldehyde (FA) on microbial activity and diversity was compared in rice field, leek patch, and tidal mud flat soil samples. MTBE, TBA and FA with different concentrations were added into microcosms containing these soil samples, and placed at room temperature for 30 days. Then the microbial activities such as dehydrogenase and viable cell numbers and microbial community using a DGGE (Denaturing gradient gel electrophoresis) fingerprinting method were measured. Among the samples, dehydrogenase activity in rice field was inhibited the most by MTBE, TBA and FA. The toxic effect was higher according to the following orders: FA > MTBE > TBA. Dominant species in the microcosms contaminated with MTBE, TBA and FA were Chloroflex, Bacilli, gamma-proteobacteria in the rice field sample, Sphingobacteria, Flavobacteria, Actinobacteria, Bacilli, gamma-proteobacteria in the leek patch sample, and Sphingobacteria, Flavobacteria, delta-proteobacteria, gamma-proteobacteria in the tidal mud flat sample.
Abstract
Toxic effect of methyl tert-butyl ether (MTBE), tert-butyl alcohol (TBA) and formaldehyde (FA) on microbial activity and diversity was compared in rice field, leek patch, and tidal mud flat soil samples. MTBE, TBA and FA with different concentrations were added into microcosms containing these soil samples, and placed at room temperature for 30 days. Then the microbial activities such as dehydrogenase and viable cell numbers and microbial community using a DGGE (Denaturing gradient gel electrophoresis) fingerprinting method were measured. Among the samples, dehydrogenase activity in rice field was inhibited the most by MTBE, TBA and FA. The toxic effect was higher according to the following orders: FA > MTBE > TBA. Dominant species in the microcosms contaminated with MTBE, TBA and FA were Chloroflex, Bacilli, gamma-proteobacteria in the rice field sample, Sphingobacteria, Flavobacteria, Actinobacteria, Bacilli, gamma-proteobacteria in the leek patch sample, and Sphingobacteria, Flavobacteria, delta-proteobacteria, gamma-proteobacteria in the tidal mud flat sample.
참고문헌 (17)
- Barbara, M., "Habitat function of agricultural soils as affected by heavy metals and polycyclic aromatic hydrocarbons contamination", Environmental Science and Pollution Research International, 28, pp.719~728 (2003)
- Bark, C., "Environmental considerations on the function and value of tidal wetland", Environmental Impact Assessment Review, 13, pp.87~101 (2004)
- Baruah, M., "Effect of herbicides butachlor, 2,4-D and oxyfluorfen on enzyme activities and CO2 evolution in submerged paddy", Plant and Soil, 96, pp.287~291 (1986)
- Bradley, P. M., "Methyl t-butyl ether mineralization in surfacewater sediment microcosms under denitrifying conditions", Applied and Environmental Microbiology, 67, pp.1975~1978 (2001)
- 조원실, "갯벌 미생물 활성 및 다양성에 미치는 Methyl tert-Butyl Ether(MTBE)와 MTBE 대사산물의 영향", 한국미생물·생명공학회지, 36(4), pp.336~342 (2008), 한국미생물·생명공학회
- CHO, WONSIL, "Bacterial Communities of Biofilms Sampled from Seepage Groundwater Contaminated with Petroleum Oil", Journal of Microbiology and Biotechnology, 15(5), pp.952~964 (2005), 한국미생물·생명공학회
- Koren, O, "Bacteria associated with mucus and tissues of the coral oculina patagonica in summer and winter", Applied and Environmental Microbiology, 72, pp.5254~5259 (2006)
- Lyew, D., "Comparison of different support materials for their capacity to immobilize Mycobacterium austroafricanum IFP 2012 and adsorb MtBE", Enzyme and Microbial Technology, 40, pp.1524~1530 (2007)
- Manusadzianas, L., "Ecotoxicological study of Lithuanian and Estonian wastewaters: selection of the biotests, and correspondence between toxicity and chemical based-based indices", Aquatic Toxicology, 63, pp.27~41 (2006)
- Natalya, I., "Effect of heavy metals and PAH on soil assessed via dehydrogenase assay", Environmental Science and Pollution Research International, 28, pp.779~782 (2003)
- Rosell, M., "Stimultaneous determination of methyl tert-butyl ether, its degradation products and other gasoline additives in soil samples by closed-system purge-and-trap gas chromatography-mass spectrometry", Journal of Chromatography A, 1132, pp.28~38 (2006)
- Rossner, A, "MTBE adsorption on alternative adsorbents and packed bed adsorb performance", Water Research, 42, pp.2287~2299 (2008)
- Steenwerth, K. L., "Soil microbial community composition as affected by restoration practices in California grassland", Soil Biology and Biochemistry, 37, pp.2249~2262 (2005)
- Torsten, C. S., "Microbial degradation of methyl tertbutyl ether and tert-butyl alcohol in the subsurface", Journal of Contaminant Hydrology, 70, pp.173~203 (2004)
- Vieira, P. A., "Biodegradation of effluent contaminated with diesel fuel and gasoline", Journal of Hazardous Materials, 140, pp.52~59 (2007)
- Wobus, A., "Microbial diversity and functional characterization of sediments from reservoirs of different trophic state", FEMS Microbiology Ecology, 46, pp.331~347 (2001)
- Zhang, Q., "Ecological effects of crude oil residues on the functional diversity of soil microorganisms in three weed rhizospheres", Journal of Environmental Sciences, 18, pp.1101~1106 (2006)