미역의 급여 수준이 타고난 면역반응이 활성화한 육계병아리의 혈액 항산화 균형에 미치는 영향
Effect of Dietary Brown Seaweed Levels on the Antioxidant System in Broiler Chicks Activated Innate Immune Response
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사료 중 미역제품 수준이 타고난 면역 반응이 활성화한 육계 병아리의 혈액내 항산화 효소계에 미치는 영향을 조사하였다. 갓 부화한 숫 병아리(Ross)에 미역제품이 0.0%(기초사료), 1.0, 2.0 및 4.0% 함유한 실험사료를 4주간 급여하였다. 타고난 면역반응은 Salmonella typhymurium lipopolysaccharide(LPS)를 8, 10 및 12 일령에 복강내에 주입하여 활성화 하고 혈액중의 과산화물, 과산화물 분해효소, 적혈구 중의 Manganese superoxide dismutase(MnSOD) 및 Copper/zinc SOD(CuZnSOD)의 활성을 측정하였다. 타고난 면역반응의 활성화는 적혈구 세포액의 SOD 활성을 유의하게(p<0.01) 높였고, 미역제품 1.0 및 2.0% 사료는 SOD 활성을 유의하게(p<0.05) 낮추었다. 타고난 면역반응이나 미역제품 사료는 혈장 과산화물 농도와 과산화물 분해효소의 활성을 높였고(p<0.01), 과산화물 농도는 미역제품의 농도(p=0.009)에 따라 증가하였다. 사료 중 미역제품 수준이 높아지면, 4 주령 육계 병아리의 혈장 과산화물 함량은 점차 타고난 면역반응 활성화 경험은 낮추고 정상 병아리는 높였다. 한편 미역제품 사료는 혈장 과산화물 분해효소 활성을 기초사료보다 타고난 면역반응 활성화 경험이 있는 것에서는 높이고(p<0.05) 정상 병아리에서는 낮추었다(p<0.05). 본 성적은 미역제품 사료와 타고난 면역 활성화는 항산화계에 영향을 미치며, 항산화계에 미치는 미역제품 2.0% 사료의 영향은 다른 사료와 다르다는 것을 나타내었다.
Abstract
Effect of dietary brown seaweed(Undaria pinnatifida) levels on the anti-oxidant enzyme system was evaluated in blood of broiler chicks activated innate immune response. Day-old broiler chicks were fed diets containing 0.0(basal), 1.0, 2.0 and 4.0% of brown seaweed for 4 weeks. The innate immune response was activated by injecting Salmonella typhymurium lipopolysaccharide(LPS) i.p. at 8, 10 and 12 day of age. The activation of innate immune response enhanced(p<0.01) and the brown seaweed 1.0 and 2.0% diets reduced(P<0.05) the superoxide dismutase(SOD) activity in erythrocyte cytosol significantly. The activation of innate immune response elevated significantly the levels of peroxide and the activity of peroxidase in plasma, while the levels of dietary brown seaweed resulted in a significant linear increase in peroxidase activity in plasma of normal bird. Experience of the innate immune response in 4 week-old chicks reduced linearly the plasma level of peroxide with the level of brown seaweed and enhanced the plasma peroxidase activity. Also, the plasma of normal birds fed the brown seaweed showed higher level of peroxide and lower activity of peroxidase. The results indicated that dietary brown seaweed affected SOD and peroxidase activities in blood of broiler chicks during activation of innate immune response.
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