본문 바로가기
KERD
논문

사료 중 미역은 복강내 LPS 주입 육계 병아리에서 영양소 대사, 항산화계 및 면역 반응을 조정한다

Dietary Brown Seaweed Modulates Nutrient Metabolism, Anti-oxidant System and Immune Response in Broiler Chicks Injected LPS i.p.

최도열|임진택|고태송|박인경
한국축산학회 (Korean Society of Animal Sciences and Technology)|2008.04|한국축산학회지 (Journal of Animal Science and Technology)|제50권 제2호|pp. 185-198

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

Lipopolysaccharide(LPS)Inflammatory cytokinesBrown seaweedProtein and energy metabolismAnti-oxidant system
주제분류축산학

초록

Influences of dietary brown seaweed(BSW) on the nutrient metabolism, anti-oxidant enzyme activity and cell-mediated immune response were studied in broiler chicks activated acute phase response. 72 Hatched male broiler chicks(Ross) were divided into 12 pens, 6 heads per pen, and fed the BSW 0.0% (Basal) or 2.0% diet, respectively, and injected with the Salmonella typhimurium lipopoly saccharide(LPS) for activation of the acute phase response three times at 8, 10 and 12 d of age. During 4 wks of experimental feeding, growth performance of broiler chicks was not affected by dietary BSW and the acute phase response. Compared with control birds, the acute phase response did not affect the daily weight gain in birds fed BSW 2.0% diet, decreased nitrogen balance(NB) or metabolizable energy(ME) utilization per metabolic body size(kg0.75), and enhanced activities of peroxidase or extracellular SOD(EcSOD), tumor necrosis factor-alpha and ovotransferrin in plasma and MnSOD and CuZnSOD in erythrocyte cytosol. Compared to BSW 0.0% diet, 2.0% diet enhanced protein retention(NB) per kg0.75 regardless the acute phase response, did not affect uric acid nitrogen excretion(UAN) per kg0.75 in birds during the acute phase response, decreased(p<0.05) the UAN excretion per kg0.75 in control birds. And BSW 2.0% diet also decreased(p<0.05) plasma peroxide level and erythrocyte peroxidase or MnSOD activity but increased plasma peroxidase and EcSOD activity and interleukin-1 activity secreted from LPS-stimulated PBMC in 4 week broiler chicks.

Abstract

Influences of dietary brown seaweed(BSW) on the nutrient metabolism, anti-oxidant enzyme activity and cell-mediated immune response were studied in broiler chicks activated acute phase response. 72 Hatched male broiler chicks(Ross) were divided into 12 pens, 6 heads per pen, and fed the BSW 0.0% (Basal) or 2.0% diet, respectively, and injected with the Salmonella typhimurium lipopoly saccharide(LPS) for activation of the acute phase response three times at 8, 10 and 12 d of age. During 4 wks of experimental feeding, growth performance of broiler chicks was not affected by dietary BSW and the acute phase response. Compared with control birds, the acute phase response did not affect the daily weight gain in birds fed BSW 2.0% diet, decreased nitrogen balance(NB) or metabolizable energy(ME) utilization per metabolic body size(kg0.75), and enhanced activities of peroxidase or extracellular SOD(EcSOD), tumor necrosis factor-alpha and ovotransferrin in plasma and MnSOD and CuZnSOD in erythrocyte cytosol. Compared to BSW 0.0% diet, 2.0% diet enhanced protein retention(NB) per kg0.75 regardless the acute phase response, did not affect uric acid nitrogen excretion(UAN) per kg0.75 in birds during the acute phase response, decreased(p<0.05) the UAN excretion per kg0.75 in control birds. And BSW 2.0% diet also decreased(p<0.05) plasma peroxide level and erythrocyte peroxidase or MnSOD activity but increased plasma peroxidase and EcSOD activity and interleukin-1 activity secreted from LPS-stimulated PBMC in 4 week broiler chicks.

참고문헌 (42)

  1. Benson, B. N., "Dietary energy source and desity modulate the expression of immunologic stress in chicks", J. Nutr, 123(10), pp.1714~1723 (1993)
  2. Bradford, MM, "A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding", Analytical Biochemistry, 72, pp.248~254 (1976)
  3. Dascombe, M. J., "Pyropgenic and ther- mogenic effects of interleukin-1β in the rat", Am. J. hysiol, 256, pp.E7~E11 (1989)
  4. Florer, E. A., "Infusion of tumor necrosis factor", cachectin promotes muscle catabolism in the rat, 1989, pp.83, 1614~1622
  5. Flick, D. A., "Comparison of In vitro cell cytotoxic assays for tumor nrcrosis factor", J. Immuno. Meth, 68, pp.167~175 (1984)
  6. Gaby, C., "Acute phase proteins and other systemic responses to inflam- mation", N. Engl. J. Med, 340, pp.448~454 (1999)
  7. Gauldie, J., "Cytokines and acute phase protein synthesis, In Cytokines and inflammation", pp.275~305 (1991), CRC Press
  8. Halliwell, B., "Exercise: an oxidative stress. In ‘Free Radicals in Biology and Medicine Third Ed" (2000), Oxford University Press
  9. Hallquist, N. A., "Serotransferrin, ovotransferrin, and metallothionein levels during an immune response in chicks", Comp. Biochem. Physiol. B-Biochem. Mol Biol, 108, pp.375~384 (1994)
  10. Hill, F. W., "Comparison of metabolizable energy and productive energy determinations with growing chicks", J. Nutr, 64, pp.587~604 (1958)
  11. Humphrey, B. D., "Modulation of nutrient metabolism and homeostasis by the immune system", World’s poultry science journal, 60 (2004)
  12. 임진택, "살모넬라 를 주입한 육계 병아리의 생산성과 질소밸런스 및 대사에너지 이용성에 미치는 사료 중 크릴 밀의 영향", 한국동물자원과학회지, 45(6), pp.957~966 (2003), 한국동물자원과학회
  13. 임진택, "살모넬라 LPS를 주입한 육계병아리에 있어서 사료 중 크릴 밀 수준이 생산성과 면역반응에 미치는 영향", 한국동물자원과학회지, 49(2), pp.225~238 (2007), 한국동물자원과학회
  14. Klasing, K. C., "Changes in protein degradation in chickens due to immunologic stress", Proc. Soc. Exp. Biol. Med, 176, pp.292~296 (1984)
  15. Klasing, K. C., "Influence of cell sources, stimulating agents, and incubation conditions on release of interleukin-1 from chicken macrophages", Develop. And Comp. Immunol., 11, pp.385~394 (1987)
  16. Klasing, K. C., "Nutritional aspects of leukocytic cytokines", Journal of Nutrition, 118, pp.1436~1446 (1988)
  17. Klasing, K. C., "dietary fish oil alters specific and inflammatory immune responses in chicks", J. Nutr, 127(10), pp.2039~2046 (1997)
  18. Klasing, K. C., "Avian macrophage: Regulators of local and systemic immune response", Poult. Sci, 77, pp.983~989 (1998)
  19. Kleiber, M., "Body size and metabolic rate", Physiol. Rev., 27, pp.511~541 (1947)
  20. Koh, T. S., "Concurrent bioassay of energy and protein utilization of protein sources in layer", K. J. Poultry. Sci, 21(2), pp.133~138 (1994)
  21. Koh, T. S., "Dietary copper level affects copper metabolism during lipopolysaccharide-induced immunological stress in chicks", Poult. Sci, 75, pp.867~872 (1996)
  22. 고태송, "급성기 반응중인 육계 병아리의 생산성에 미치는 사료중 크릴 밀의 영향", 한국동물자원과학회지, 46(2), pp.173~182 (2004), 한국동물자원과학회
  23. 고태송, "급성기 반응을 활성화한 육계 병아리에서 사료중 미역 제품 수준이 단백질과 에너지 대사에 미치는 영향", 한국동물자원과학회지, 47(3), pp.379~390 (2005), 한국동물자원과학회
  24. Koutsos, E. A., "Carotenoids from in ovo or dietary sources blunt systemic indices of the inflammatory response in growing chicks", J. Nutr, 136, pp.1027~1031 (2006)
  25. Kushner, I., "Acute phase response. Inflammation: Basic principles and Clinical Correlates", pp.317~330 (1999), Lippincot-Williams & Wilkins
  26. Lam, K. M., "Myeloperoxidase activity in chicken heterophils and adherent cells", Vererinary Immunology and Immunopathology, 3-4, pp.327~335 (1997)
  27. Lee, D. S., "Uronate compositions of alginates from the edible brown algaer", Han`guk Susan Hakhoechi, 31(1), pp.1~7 (1998)
  28. 이혜정, "미역의 급여 수준이 타고난 면역반응이 활성화한 육계병아리의 혈액 항산화 균형에 미치는 영향", 한국동물자원과학회지, 47(1), pp.29~28 (2005), 한국동물자원과학회
  29. Leshchinsky, T. V., "Divergence of the inflammatory response in two types of chickens", Developmental & Comparative Immunology, 25, pp.629~638 (2001)
  30. Marklund, S., "Involvement of the superoxide anion radical in the auto- oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J. Biochem, 47, pp.469~474 (1974)
  31. Marquardt, R. R., "A simple spectro- photometric method for the direct determination of uric acid in avian excreta", Poult. Sci, 62, pp.2106~2108 (1983)
  32. McCord, J. M., "Superoxide dismutase. An enzyme function for erythrocuprein(hemocuperin)", J. Biol. Chem, 244, pp.6049~6055 (1969)
  33. Otterlei, M., "Induction of cytokine production from human monocytes stimulated with alginate", J. Immunother, 10, pp.286~291 (1991)
  34. 박인경, "사료중 크릴 밀을 급여한 육계의 생산성과 SOD활성에 미치는 급성기반응의 영향", 한국동물자원과학회지, 46(2), pp.183~192 (2004), 한국동물자원과학회
  35. Roura, E., "Prevention of immunologic stress contributes to the growth-permitting ability of dietary antibiotics in chicks", J. Nutr, 122, pp.2383~2390 (1992)
  36. SAS. Institute Inc., "SAS user’s Guide, statistics version 5 ed", SAS Institute Inc., ~ (1988)
  37. Suffredini, A. F., "New insights into the biology of the acute phase response", J. Clin. Immunol, 19, pp.203~214 (1999)
  38. Tatzber, F., "Dual method for the deter- mination of peroxidase activity and total peroxides-iodide leads to a significant increase of peroxidase activity in human sera", Analytical Biochemistry, 316, pp.147~153 (2003)
  39. Tian, S., "Effect of Escherichia coli infection on growth and protein metabolism in broiler chicks(Gallus domesticus)", Comparative Biochemistry & Physiology part A, 94, pp.323~331 (1989)
  40. Tian, S., "Prostaglandin- dependent muscle wasting during infection in the broiler chick(Gallus domesticus) and the laboratory rat(Rattus norvegicus)", Biochem Journal, 263, pp.485~490 (1989)
  41. 동물용 발열량 자동 측정 장치의 시험 제작에 관한 연구, "고태송", 한국 과학 재단, 1~53, pp.KOSEF 901-1505-074-1 (1993)
  42. 최철림, "육계 병아리에서 사료 중 대두유는 면역 스트레스를 완화한다", ’95 축산분야 종합 학술 대회, pp.283~ (1995)

이 간행물의 다른 논문 (14건)

Estimation of Genetic Parameters of Body Weights in Hanwoo Steers(Korean Cattle), Bos Taurus Coreanae Using Random Regression ModelAgapita J. Salces, 서강석, 최태정, 윤두학, 이홍구, 김상훈pp. 151-156한우 거세우 도체중 및 체형형질의 유전모수 추정에 관한 연구신재영, 김병우, 이정규, 이종헌, 송치은, 조병대, 구양모, 이재윤, 김정일pp. 157-166Microsatellite loci 분석을 통한 한우 지역 브랜드간 유전적 다양성의 비교서강석, 양대용, 상병찬, 이기환, 최태정, 윤두학pp. 167-176미생물에서 돼지 150-kDa Insulin-Like Growth Factor Complex의 Acid-Labile Subunit 발현강혜경, 문양수, 이철영pp. 177-184보호아미노산의 추가 공급이 반추위 발효성상 및 비유중기 착유우의 유량 및 유성분에 미치는 영향이종민, 남인식, 안종호pp. 199-208
원문 보기