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元氣生脈散의 추출물별 항산화작용과 쥐의 운동피로 회복효과

Effect of Wongisaengmaek-san Extract on Antioxidative And Anti-Fatigue Activity

류봉하|김진성|김유승
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2009.03|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제30권 제1호|pp. 94-106

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키워드

AntioxidantWongisaengmaek-sanAnti-Fatigue
주제분류한의학

초록

Objectives : This study was aimed to evaluate the effect of Wongisaengmaek-San which was extracted from water or 50% ethanol on antioxidative activity and recovery of fatigue induced by weight-loaded forced swimming exercise. Methods : Antioxidant activity of Wongisaengmaek-San was evaluated in terms of total amount of polyphenol, 1,1-diphenyl-2-picrylhydroxyl (DPPH) radical scavenging activity and xanthine oxidase inhibition. The anti-fatigue effect of Wongisaengmaek-San was investigated using an acute weight-loaded foreced swimming test by monitoring swimming test times, blood biochemical parameters as creatinine, BUN, lactic acid dehydrogenase(LDH), free fatty acid(FFA) and Lactic acid(LA). Results : 1. 50% EE (ethanol extract) had 2.3 times higher amount of tatal polyphenol compared to water extract. 2. It has been identified that 50% ethanol extract showed enhancement of xanthine oxidase inhibition and DPPH scavenging effect in vitro. 3. According to anti-fatigue effect in the scale of 5% weight loaded mouse swimming test, both water and 50% ethanol extract was approved to show significant improvement of swimming time elongation respectively, and 50% ethanol induced positive result. 4. After swimming for 1% weight-loaded mouse, water extract and 50% ethanol showed significantly anti-fatigue effect with manifestation of blood sampling among two of the intervention, 50% ethanol showed positive result. Conclusions : 50% ethanol extract of Wongisaengmaek-San has more effective antioxidant activity and anti-fatigue than water extract.

Abstract

Objectives : This study was aimed to evaluate the effect of Wongisaengmaek-San which was extracted from water or 50% ethanol on antioxidative activity and recovery of fatigue induced by weight-loaded forced swimming exercise. Methods : Antioxidant activity of Wongisaengmaek-San was evaluated in terms of total amount of polyphenol, 1,1-diphenyl-2-picrylhydroxyl (DPPH) radical scavenging activity and xanthine oxidase inhibition. The anti-fatigue effect of Wongisaengmaek-San was investigated using an acute weight-loaded foreced swimming test by monitoring swimming test times, blood biochemical parameters as creatinine, BUN, lactic acid dehydrogenase(LDH), free fatty acid(FFA) and Lactic acid(LA). Results : 1. 50% EE (ethanol extract) had 2.3 times higher amount of tatal polyphenol compared to water extract. 2. It has been identified that 50% ethanol extract showed enhancement of xanthine oxidase inhibition and DPPH scavenging effect in vitro. 3. According to anti-fatigue effect in the scale of 5% weight loaded mouse swimming test, both water and 50% ethanol extract was approved to show significant improvement of swimming time elongation respectively, and 50% ethanol induced positive result. 4. After swimming for 1% weight-loaded mouse, water extract and 50% ethanol showed significantly anti-fatigue effect with manifestation of blood sampling among two of the intervention, 50% ethanol showed positive result. Conclusions : 50% ethanol extract of Wongisaengmaek-San has more effective antioxidant activity and anti-fatigue than water extract.

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