牧丹皮가 손상된 성상신경세포의 CD81 및 GFAP의 발현에 미치는 영향
The Effect of The Moutan Radicis Cortex on Expression of CD81 and GFAP in Injured Astrocyte
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Object : In condition of brain infarction, irreversible axon damage occurs in central nerve system (CNS), because gliosis makes physical and mechanical barrier. If gliosis formation could be suppressed, irreversible axon damage would be reduced. This could mean that injured CNS could be regenerated. CD81 and GFAP has close relation to gliosis. The increase in glial cells at injured CNS gives rise to the expression of CD81 and GFAP. CD81 was postulated to play a central role in the process of CNS scar formation. Method : So in this study, the author investigated the effect of the water extract of the Moutan Radicis Cortex on regulation of CD81 and GFAP expression in injured CNS cells. MTT assay was used to examine cell viability, and RT-PCR and ELISA methods were carried out to measure the expression of CD81 and GFAP in the astrocyte. Results : We observed that water extract of the Moutan Radicis Cortex increased cell viability under hypoxia induced by CoCl2 and suppressed the expression of CD81 and GFAP up-regulated by hypoxia. Conclusion : In conclusion, these results suggest that the Moutan Radicis Cortex could promote neural regeneration as a consequence of protecting CNS cells from hypoxia and suppressing the reactive gliosis following CNS injury
Abstract
Object : In condition of brain infarction, irreversible axon damage occurs in central nerve system (CNS), because gliosis makes physical and mechanical barrier. If gliosis formation could be suppressed, irreversible axon damage would be reduced. This could mean that injured CNS could be regenerated. CD81 and GFAP has close relation to gliosis. The increase in glial cells at injured CNS gives rise to the expression of CD81 and GFAP. CD81 was postulated to play a central role in the process of CNS scar formation. Method : So in this study, the author investigated the effect of the water extract of the Moutan Radicis Cortex on regulation of CD81 and GFAP expression in injured CNS cells. MTT assay was used to examine cell viability, and RT-PCR and ELISA methods were carried out to measure the expression of CD81 and GFAP in the astrocyte. Results : We observed that water extract of the Moutan Radicis Cortex increased cell viability under hypoxia induced by CoCl2 and suppressed the expression of CD81 and GFAP up-regulated by hypoxia. Conclusion : In conclusion, these results suggest that the Moutan Radicis Cortex could promote neural regeneration as a consequence of protecting CNS cells from hypoxia and suppressing the reactive gliosis following CNS injury
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