콜라비가 돼지 지방전구세포와 3T3-L1 cell의 증식과 분화에 미치는 영향
Effects of Kohlrabi(Brassica oleracea var. Gongylodes) on Proliferation and Differentiation of Pig Preadipocytes and 3T3-L1 Cells
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본 연구는 콜라비가 돼지 지방전구세포와 3T3-L1 세포의 증식과 분화에 미치는 영향을 구명하기 위해 수행하였다. 돼지 지방전구세포는 신생자돈의 등지방에서 분리했다. 세포를 접종한 1일 후에 세척했고(day 0), 세포증식에 미치는 영향을 구명하기 위해서 2일 동안(day 0∼day 2) 25ng/ml과 100ng/ml의 콜라비 알코올 추출 물(과피와 과육)를 처리했다. 세포분화를 구명하기 위해서는 DMEM/F- 12 배지에 6일 동안(day 0∼day 6) 배양하고 배양초기 2일 동안(day 0~day 2) 콜라비를 처리하고 day 6에 세포 분화를 측정했다. 콜라비 과피 25ng/ml와 100ng/ml은 돼지 지방전구세포의 증식을 각각 4.59%, 17.7% 억제했고, 콜라비 과육은 각각 11.4%, 19.2% 억제했다. 반면 돼지 지방전구세포의 분화는 억제하지 않았다. 콜라비가 3T3-L1 cell의 증식과 분화에 미치는 작용을 구명하기 위해, 돼지 지방전구세포처럼, 세포 배양초기 2일간 콜라비를 처리했는데 콜라비 과피와 과육 둘 다 세포의 증식과 분화에 영향을 미치지 않았다. 본 연구의 결과를 요약하면, 콜라비는 돼지 지방전구세포의 증식을 억제했으나 분화는 억제하지 않았고, 한편 3T3-L1 cell의 증식과 분화 모두 영향을 미치지 않았다.
Abstract
The current study was carried out to determine the effects of Kohlrabi(Brassica oleracea var. gongylodes) on proliferation and differentiation of pig preadipocytes and 3T3-L1 cells. Pig preadipocytes were isolated from the backfat of the new-born pigs. Twenty-four hours after seeding, the cells were washed with DMEM/F-12(designated day 0). To measure the cell proliferation, the cells were treated with 25ng/ml and 100 ng/ml ethanol extracts of Kohlrabi(peel and flesh) for two days(day 0~2). To measure differentiation, the cells were treated with Kohlrabi for two days(day 0~2) and cell differentiation was measured on day 6. Twenty-five ng/ml and 100ng/ml of Kohlrabi peel decreased proliferation of pig preadipocytes by 4.59% and 17.7%, respectively, compared with the control and Kohlrabi flesh by 11.4% and 19.2%, respectively. However, Kohlrabi did not inhibit cell differentiation. To measure the effects of Kohlrabi on proliferation and differentiation of 3T3-L1 cells, the cells were treated with Kohlrabi for two days in culture, like pig preadipocytes. Kohlrabi(both peel and flesh) did not show any effects on cell proliferation and differentiation. In summary, the results of the current study showed that Kohlrabi decreased proliferation of pig preadipocytes, but no inhibitory effects on differentiation of the cells. Kohlrabi had no effects on proliferation and differentiation of 3T3-L1 cells.
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