어류 병원성 세균의 MIC 결정을 위한 MIC Panel의 최적화 배양 조건 확립
Establishing of Optimal Culture Conditions for MIC Panels for MIC Determination of Fish Bacterial Pathogens
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초록
No established method can be used to select effective antibiotics in antibiotic susceptibility tests for fish bacterialpathogens quickly and accurately. Here, we established the optimal conditions for determining the minimal inhibitoryconcentration (MIC) of major fish bacterial pathogens (Streptococcus spp., Edwardsiella tarda, Vibrio spp., Aeromonasspp., and Pseudomonas spp.) using the KRAQ1 and CAMPY2 panels. The MIC panel used 18 antibiotics of twotypes and we conducted experiments to establish the optimal culture medium and temperature for each species. Theoptimal conditions for incubating Streptococcus spp. were in cation-adjusted Mueller-Hinton broth with TES buffer(CAMHBT) at 28°C, using 5% lysed horse blood (LHB) as recommended by the Clinical Laboratory StandardsInstitute. For Vibrio spp., the optimal culture conditions were 28°C in CAMHBT supplemented with 1% NaCl. Theoptimal conditions for culturing E. tarda, Aeromonas spp., and Pseudomonas spp. were in CAMHBT at 28°C
Abstract
No established method can be used to select effective antibiotics in antibiotic susceptibility tests for fish bacterialpathogens quickly and accurately. Here, we established the optimal conditions for determining the minimal inhibitoryconcentration (MIC) of major fish bacterial pathogens (Streptococcus spp., Edwardsiella tarda, Vibrio spp., Aeromonasspp., and Pseudomonas spp.) using the KRAQ1 and CAMPY2 panels. The MIC panel used 18 antibiotics of twotypes and we conducted experiments to establish the optimal culture medium and temperature for each species. Theoptimal conditions for incubating Streptococcus spp. were in cation-adjusted Mueller-Hinton broth with TES buffer(CAMHBT) at 28°C, using 5% lysed horse blood (LHB) as recommended by the Clinical Laboratory StandardsInstitute. For Vibrio spp., the optimal culture conditions were 28°C in CAMHBT supplemented with 1% NaCl. Theoptimal conditions for culturing E. tarda, Aeromonas spp., and Pseudomonas spp. were in CAMHBT at 28°C
참고문헌 (19)
- 천원경, "Streptococcus parauberis의 디스크 확산법 결과에 대한 Epidemiological Cut-off Value의 설정", 한국수산과학회지, 52(4), pp.382~388 (2019), 한국수산과학회[DOI]
- Method for antimicrobial disk susceptibility testing of bacteria isolated from aquatic animals, "CLSI", CLSI, ~, pp.CLSI document M42-A (2006)
- Methods for broth dilution susceptibility testing of bacteria isolated from aquatic animals, "CLSI", CLSI, ~, pp.CLSI document VET04-A2 (2014)
- Performance standards for antimicrobial susceptibility testing of bacteria isolated from aquatic animals; Second informational supplement, "CLSI", CLSI, ~, pp.CLSI document VET03 (2014), VET04-S2
- Performance standards for antimicrobial susceptibility testing; thirtieth informational supplement, "CLSI", CLSI, ~, pp.CLSI document M100-ED30 (2020)
- Demircan D, "Identification of Vibrio anguilliarum by PCR (rpoN Gene) associated with Vibriosis in marine fish in Turkey", Turk J Vet Anim Sci, 30, pp.305~310 (2006)
- "Antimicrobial susceptibility testing EUCAST disk diffusion method, Version 8.0" (2020)[URL]
- Guide to drug, vaccine, and pesticide use in aquaculture, "JSA", Texas A&M University, ~, pp.Texas agricultural extension service, B-5085 (1994)
- Hyun-Joong Kim, "Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics", BMC Microbiology, 15(1), pp.239~ (2015), Springer Science and Business Media LLC[DOI]
- Myoung Sug Kim, "Identification of Vibrio harveyi, Vibrio ichthyoenteri, and Photobacterium damselae isolated from olive flounder Paralichthys olivaceus in Korea by multiplex PCR developed using the rpoB gene", Fisheries Science, 80(2), pp.333~339 (2014), Springer Science and Business Media LLC[DOI]
- 김영재, "국내 수산용의약품의 관리 현황 모니터링", 한국어병학회지, 32(1), pp.37~43 (2019), 한국어병학회[DOI]
- 이다원, "2016년도 제주지역 양식 넙치(Paralichthys olivaceus)에서 분리된 어병세균의 tetracycline 내성유전자 분포", 수산해양교육연구, 29(3), pp.834~846 (2017), 한국수산해양교육학회[DOI]
- Antibiotic resistant bacteria inspection manual, "NIFS", GMK communication, 34~49, pp.11-1192266-000195-01 (2017)
- NIFS, "Aquatic medicine catalog", pp.12~101 (2018), Aquatic Disease Control Division
- Noga EJ, "Fish disease", pp.19~215 (1996), Mosby-Year Book, Inc.
- Takamitsu Sakai, "Detection of Type 1 Fimbrial Genes in Fish Pathogenic and Non-pathogenic Edwardsiella tarda Strains by PCR", Fish Pathology, 42(2), pp.115~117 (2007), The Japanese Society of Fish Pathology[DOI]
- 손광태, "양식 넙치(Paralichthys olivaceus )에 경구투여한 Ampicillin 및 Amoxicillin의 어류체내 잔류량의 변화", 한국수산과학회지, 44(5), pp.464~469 (2011), 한국수산과학회
- 우승호, "해수 양식 어류에서 분리된 연쇄상구균의 종류와 병원성", 한국어병학회지, 19(1), pp.17~33 (2006), 한국어병학회
- Woodward KN, "The regulation of fish medicines-UK and European Union aspects", Aquac Res, 27, pp.725~734 (1996)[DOI]