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Streptococcus parauberis의 디스크 확산법 결과에 대한 Epidemiological Cut-off Value의 설정

Epidemiological Cut-off Values Generated for Disc Diffusion Data from Streptococcus parauberis

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주제분류수산학

초록

Streptococcosis caused by Streptococcus parauberis is a very important disease in farmed olive flounder Paralichthys olivaceus. For most fish pathogens, including S. parauberis, there are no analytical criteria to distinguish antibioticsusceptible strains from antibiotic-resistant strains. In this study, epidemiological cut-off (COWT) values were generated to classify 75 strains of S. parauberis isolated from 1999 to 2018 as wild type (WT) and non-wild type (NWT) using disc diffusion data and normalized resistance interpretation (NRI) analysis. The susceptibility of the isolates to 16 antibiotics was evaluated using CLSI guideline M42-A. The wild-type cut-off values for amoxicillin, erythromycin, oxytetracycline, and florfenicol for S. parauberis were ≥ 35, 31, 28, and 27 mm, respectively. The NWT ratios of S. parauberis strains to treatment with GEN, FFC, ENR, SXT, EFT, VAN, and CHL were 17% or less, indicating that these antibiotics may be used to treat streptococcosis caused by S. parauberis. For recent S. parauberis isolates, the NWT ratios for AMX, ERY, OTC and FFC are much higher than for strains isolated from 1999-2007. The COWT data from this study will assist aquatic animal disease professionals in prescribing appropriate antibiotics for the treatment of streptococcosis caused by S. parauberis, which will help reduce the misuse and abuse of antibiotics in the aquaculture sector.

Abstract

Streptococcosis caused by Streptococcus parauberis is a very important disease in farmed olive flounder Paralichthys olivaceus. For most fish pathogens, including S. parauberis, there are no analytical criteria to distinguish antibioticsusceptible strains from antibiotic-resistant strains. In this study, epidemiological cut-off (COWT) values were generated to classify 75 strains of S. parauberis isolated from 1999 to 2018 as wild type (WT) and non-wild type (NWT) using disc diffusion data and normalized resistance interpretation (NRI) analysis. The susceptibility of the isolates to 16 antibiotics was evaluated using CLSI guideline M42-A. The wild-type cut-off values for amoxicillin, erythromycin, oxytetracycline, and florfenicol for S. parauberis were ≥ 35, 31, 28, and 27 mm, respectively. The NWT ratios of S. parauberis strains to treatment with GEN, FFC, ENR, SXT, EFT, VAN, and CHL were 17% or less, indicating that these antibiotics may be used to treat streptococcosis caused by S. parauberis. For recent S. parauberis isolates, the NWT ratios for AMX, ERY, OTC and FFC are much higher than for strains isolated from 1999-2007. The COWT data from this study will assist aquatic animal disease professionals in prescribing appropriate antibiotics for the treatment of streptococcosis caused by S. parauberis, which will help reduce the misuse and abuse of antibiotics in the aquaculture sector.

참고문헌 (34)

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Photobacterium damselae의 디스크 확산법 결과에 대한 Epidemiological Cut-off Value의 결정권문경, 김도형, 김명석, 서정수, 임윤진한국수산과학회 · 한국수산과학회지2016 · 제49권 제6호Application of tylosin antibiotics to olive flounder (Paralichthys olivaceus) infected with Streptococcus parauberis권문경, 김윤재, 박찬일, 서정수, 이지훈, 정지민, 주민수, 황성돈, 황지연, Hee-Chung Lee, Kwang-Min Choi한국수산과학회 · Fisheries and Aquatic Sciences2020 · 제23권 제3호제주지역 넙치(Paralichthys olivaceus) 연쇄구균병 원인체의 분리특성과 Streptococcus parauberis의 혈청형 변화강봉조, 김경욱, 양혜영, 유은호수산생명의학회 · 한국어병학회지2020 · 제33권 제2호제주지역 양식 넙치(Paralichthys olivaceus)에서 분리한 어병세균 내Erythromycin 내성 유전자 분석김승민, 이다원, 전려진, 정준범한국수산과학회 · 한국수산과학회지2018 · 제51권 제4호양식 넙치에서 분리한 Streptococcus parauberis의 동정방법에 따른 지역적 비교김재훈, 박명애, 오윤경, 이덕찬, 조미영수산생명의학회 · 한국어병학회지2007 · 제20권 제1호양식 넙치, Paralichthys olivaceus에서 분리된 Edwardsiella tarda, Vibrio spp., Streptococcus spp.의 항균제 내성 경향권문경, 김명석, 박명애, 서정수, 정승희, 조지영, 황지연수산생명의학회 · 한국어병학회지2010 · 제23권 제1호

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