3,4-Dichloroaniline 분해 미생물의 분리 및 특성
Isolation and Characterization of 3,4-Dichloroaniline Degrading Bacteria
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
토양 시료를 대상으로 3.4-dichloroaniline (DCA)를 함유한 최소배지에서의 집식배양과 배양 후 HPLC에 의한 잔류분석을 통해 3,4-DCA의 분해 능력이 우수한 균주 Pseudomonas sp. KB35B를 분리하였다. 분리균 KB35B는 1/10 LB배지에 함유된 50 ppm의 3,4-DCA를 12시간만에 완전히 제거하였다. 이외에도 분리균 KB35B는 3-chloroaniline (CA), 4-CA 및 2,4-DCA의 분해 활성을 나타내었으나 2,5-DCA와 3,5-DCA에 대한 분해활성을 가지고 있지는 않았다. 또한, 분리균 KB35B에서 3,4-DCA의 유도에 의한 catechol 2,3-dioxygenase 활성의 증가가 관찰되었다. 이상의 결과로부터 catechol 2,3-dioxygenase이 3,4-DCA 분해에 관여하는 중요한 효소군중의 하나로 생각된다.
Abstract
Chloroanilines are widely used in the production of dyes, drugs and herbicides. Chloroanilines, however, are considered potential pollutants due to their toxic and recalcitrant properties to humans and other species. With the increase of necessity of bioremediation, this study was conducted to isolate the chloroanilines-degrading bacteria. A bacterium capable of growth on 3,4-dichloroaniline (DCA) was isolated by the 3,4-DCA-containing enrichment culture. The strain KB35B was identified as Pseudomonas sp. and also able to degrade several chloroanilines. The isolated strain showed high level of catechol 2,3-dioxygenase activity in the presence of 3,4-DCA. The activity of catecho1 2,3-dioxygenase was supposed to be ones of the important factors for 3,4-DCA degradation. The activity toward 4-methykatechol was 60.6% of that of catechol, while the activity toward 3-methylcatechol and 4-chlorocatechol were 27.0 and 13.5%, respectively.
참고문헌 (17)
- Aoki, "Purification and characterization of catechol 1 2-dioxygenase from aniline-assimilating Rhodococcus erythropolis AN-13", ko(hana), pp.2087~2095 (1984)
- Dunbar J, "Assessment of microbial diversity in four Southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis", 66, pp.2943~2950 (2000)
- Gheewala S. H., "Biodegradation of aniline", 36, pp.53~63 (1997)
- Hanahan, "Studies on transformation of Escherichia coli with plasmid", pp.557~580 (1983)
- Gen, "The mata clevage operon of TOL degradative plasmid pWWO comprised 13 gene", pp.113~120 (1990)
- Kearny, "Degradation and Mode of action" (1975)
- Lee J. S, "Identification of Yarrowia lipolytica Y103 and its degradability of phenol and 4-chlorophenol", 11, pp.112~117 (2001)
- Liu Z, "Degradation of aniline by newly isolated, extremely anilinetolerant Delftia p. AN3", 58, pp.679~682 (2002)
- Motonaga K, "Biodegradation of chlorothalonil in soil after suppression of degradation", 23, pp.340~345 (1996)
- Na K, "Cloning and phylogenetic analysis of two diferent bphC genes and bphD gene from PCB-degrading bacterium, Pseudomonas sp. strain SY5", 11, pp.668~676 (2001)
- Nakanishi, "and characterization of catechol 2 3-dioxygenase from aniline-assimilating Pseudomonas sp", pp.1281~1289 (1991)
- Park D. W, "Sequence characteristics of xyl JQK genes reponsible for catechol degradation in benzoate-catabolizing Pseudomonas sp. S-47", 13, pp.700~705 (2003)
- Radianingtyas H, "Characterization of a soil-derived bacterial consortium degrading 4-chloroaniline", 149, pp.3279~3287 (2003)
- Sutherland T. D, "Enrichment of an endosulfan-degrading mixed bacterial culture", 66, pp.2822~2828 (2000)
- Tixier C, "Biotransformation of phenylurea herbicides by a soil bacterial strain, Arthrobacter sp. N2: structure, ecotoxicity and fate of diuron metabolite with soil fungi", 46, pp.519~526 (2002)
- Travkin V. M, "Degradation of 3,4-dichloro- and 3,4-difluoroaniline by Pseudomonas fluorescens 26-K", 38, pp.4 121~132 (2003)
- Hoshino, "Restriction and modification in Bacillus species Genetic transformation of bacteria with DNA from different species", hori(uchi), pp.525~538 (1977)