Isolation and Characterization of Nonylphenol-degrading Bacteria
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초록
To isolate a nonylphenol (NP)-degrading bacterium, we isolated a single colony from the NP-degrading microbial consortium SW-3, which was previously isolated from an aqueous environment. Ten colonies that exhibited different cell morphologies were isolated and the strains were named SW-3-A, -B, -C, -D, -E, -F1, -F2, -G, -H, and -I. The ability of isolates to degrade NP was evaluated by kinetic analysis by the constant of NP degradation rate (k1) and the half-life time of NP degradation (t1/2). SW-3-F1,-F2, -G, and -I strains were superior at degrading NP. The k1 and t1/2 values of the four strains were sixfold higher and one-sixth lower, respectively, than those of the consortium strain. Additionally, SW-3-F1, -G, and -I strains were tested for their ability to degrade NP during coculture. NP degradation by coculture with a combination of all three strains was inferior to that of culture conducted with single isolates, suggesting that the three strains are antagonistic toward each other during NP degradation.
Abstract
To isolate a nonylphenol (NP)-degrading bacterium, we isolated a single colony from the NP-degrading microbial consortium SW-3, which was previously isolated from an aqueous environment. Ten colonies that exhibited different cell morphologies were isolated and the strains were named SW-3-A, -B, -C, -D, -E, -F1, -F2, -G, -H, and -I. The ability of isolates to degrade NP was evaluated by kinetic analysis by the constant of NP degradation rate (k1) and the half-life time of NP degradation (t1/2). SW-3-F1,-F2, -G, and -I strains were superior at degrading NP. The k1 and t1/2 values of the four strains were sixfold higher and one-sixth lower, respectively, than those of the consortium strain. Additionally, SW-3-F1, -G, and -I strains were tested for their ability to degrade NP during coculture. NP degradation by coculture with a combination of all three strains was inferior to that of culture conducted with single isolates, suggesting that the three strains are antagonistic toward each other during NP degradation.
참고문헌 (28)
- Ahel M, "Behaviour of alkylphenol polyethoxylate surfactants in the aquatic environment-I. Occurrence and transformation in sewage treatment", Water Res, 28, pp.1131~1142 (1994)
- Chang BV, "Biodegradation of nonylphenol in soil", Chemosphere, 66, pp.1857~1862 (2007)
- 조현서, "광양만과 여수해만의 표층퇴적물에서 Nonylphenol의 오염에 관한 연구", 한국환경과학회지, 13(6), pp.561~570 (2004), 한국환경과학회
- Corvini PFX, "Degradation of a nonylphenol single isomer by Sphingomonas sp. strain TTNP3 leads to a hydroxylation-induced migration product", Appl Environ Microbiol, 70, pp.6897~6900 (2004)
- Di Gioia D, "Aggregation- based cooperation during bacterial aerobic degradation of polyethoxylated nonylphenols", Res Microbiol, 155, pp.761~769 (2004)
- Di Gioia D, "Characterization of 4-nonylphenol-degradingbacterial consortium obtained from a textile wastewater pretreatment plant", Arch Microbiol, 190, pp.673~683 (2008)
- Dunbar J, "Assessment of microbial diversity in four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis", Appl Environ Microbiol, 66, pp.2943~2950 (2000)
- Fries E, "Occurrence and behaviour of 4-nonylphenol in river water of Germany", J Environ Monit, 5, pp.598~603 (2003)
- Fujii K, "Microbial degradation of nonylphenol in some aquatic enviroments", Fish Sci, 66, pp.44~48 (2000)
- Fujii K, "Sphingomonas cloacae sp. nov., a nonylphenol-degrading bacterium isolated from wastewater of a sewage-treatment plant in Tokyo", Int J Syst Evol Microbiol, 51, pp.603~610 (2001)
- Gabriel FLP, "Differential degradation of nonylphenol isomers by Sphingomonas xenophaga Bayram", Appl Environ Microbiol, 71, pp.1123~1129 (2005)
- Giger W, "4-nonylphenol in sewage sludge: accumulation of toxic metabolites from nonionic surfactants", Science, 225, pp.623~625 (1984)
- Gronen S, "Serum vitellogenin levels and reproductive impairment of male Japanese Medaka (Oryzias latipes) exposed to 4-tertoctylphenol", Environ Health Perspect, 107, pp.385~390 (1999)
- Hernandez-Raquet G, "Removal of the endocrine disrupter nonylphenol and its estrogenic activity in sludge treatment processes", Water Res, 41, pp.2643~2651 (2007)
- Junghanns C, "Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases", Microbiology, 151, pp.45~57 (2005)
- 강민승, "연안 갯벌에서 분리한 Chloroaniline 화합물 분해 미생물의 특징", 한국수산과학회지, 40(5), pp.282~287 (2007), 한국수산과학회
- Karels AA, "Reproductive effects of estrogenic and antiestrogenic chemicals on sheepshead minnows (Cyprinodon variegatus)", Environ Toxicol Chem, 22, pp.855~865 (2003)
- 김종성, "Characterization and distribution of trace organic contaminants in sediment from Masan Bay, Korea. 1. Instrumental analysis", ENVIRONMENTAL SCIENCE & TECHNOLOGY, 33, pp.4199~4205
- Kim YM, "Glutathione- dependent biotransformation of the fungicide chlorothalonil", J Agric Food Chem, 52, pp.4192~4196 (2004)
- Kim YM, "Cloning and characterization of a catechol-degrading gene cluster from 3,4-dichloroaniline degrading bacterium Pseudomonas sp KB35B", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 55, pp.4722~4727, AMER CHEMICAL SOC
- Kuroda M, "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection", Proc Natl Acad Sci USA, 102, pp.13272~13277 (2005)
- Yun-Kyoung Lee, "Cloning and Mutational Analysis of Catechol 2,3-dioxygenase from 3,4-Dichloroaniline Degrading Bacterium Pseudomonas sp. KB35B", Journal of the Korean Society for Applied Biological Chemistry, 52(3), pp.258~263 (2009), 한국응용생명화학회
- 김민선, "Distribution characteristics of nonylphenols in the artificial Lake Shihwa, and surrounding creeks in Korea", CHEMOSPHERE, 56, pp.783~790, PERGAMON-ELSEVIER SCIENCE LTD
- Ripp S, "Controlled field release of a bioluminescent genetically engineered microorganism for bioremediation process monitoring and control", Environ Sci Technol, 34, pp.846~853 (2000)
- Shi S, "Changes to the structure of Sphingomonas spp. communities associated with biodegradation of the herbicide isoproturon in soil", FEMS Microbiol Lett, 269, pp.110~116 (2007)
- 송원, "Nonylphenol 분해 미생물 컨소시엄 균주 개발", 한국수산과학회지, 44(4), pp.325~331 (2011), 한국수산과학회
- Tanghe T, "Isolation of a bacterial strain able to degrade branched nonylphenol", Appl Environ Microbiol, 65, pp.746~751 (1999)
- Yadetie F, "Effects of 4-nonylphenol on gene expression of pituitary hormones in juvenile Atlantic salmon (Salmo salar)", Aquat Toxicol, 58, pp.113~129 (2002)