The Effects of Acute Osmotic Stress on Innate Immunity of Nile Tilapia (Oreochromis niloticus)
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The effects of osmotic stress on the non-specific immune response of Nile tilapia, Oreochromis niloticus, were investigated. Osmoregulatory mechanism of tilapia has been studied, but less information is available about innate immune response of O. niloticus faced with hyperosmolality. Acute osmotic stress was elicited by transferring tilapia from freshwater (FW) to 24 psu seawater (SW). Non-specific immune parameters including lysozyme activities of plasma and head kidney (HK), alternative complement pathway (ACP) activity in plasma, phagocytic capacities of spleen and HK immune cells, and respiratory burst activity of immune cells in both HK and spleen were analyzed. Lysozyme activities were increased at 1 h and 30 h after transfer to SW, but decreased at 10 h after SW transfer. Conversely, ACP activity increased 10 h after SW transfer. Phagocytic capacity increased slightly at 1 h and 5 h after SW transfer, and respiratory burst activity showed an increase in superoxide release at 10 h after SW transfer. Taken together, these results indicate that the exposure of tilapia to hyperosmotic conditions has immunostimulatory effects on cellular and humoral immune reactions.
Abstract
The effects of osmotic stress on the non-specific immune response of Nile tilapia, Oreochromis niloticus, were investigated. Osmoregulatory mechanism of tilapia has been studied, but less information is available about innate immune response of O. niloticus faced with hyperosmolality. Acute osmotic stress was elicited by transferring tilapia from freshwater (FW) to 24 psu seawater (SW). Non-specific immune parameters including lysozyme activities of plasma and head kidney (HK), alternative complement pathway (ACP) activity in plasma, phagocytic capacities of spleen and HK immune cells, and respiratory burst activity of immune cells in both HK and spleen were analyzed. Lysozyme activities were increased at 1 h and 30 h after transfer to SW, but decreased at 10 h after SW transfer. Conversely, ACP activity increased 10 h after SW transfer. Phagocytic capacity increased slightly at 1 h and 5 h after SW transfer, and respiratory burst activity showed an increase in superoxide release at 10 h after SW transfer. Taken together, these results indicate that the exposure of tilapia to hyperosmotic conditions has immunostimulatory effects on cellular and humoral immune reactions.
참고문헌 (25)
- Barton B, "Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids", Ann Rev Fish Dis, 10, pp.3~26 (1991)
- Craig S, "Meconium inhibits phagocytosis and stimulates res-piratory burst in alveolar macrophages", Ped Res, 57, pp.813~818 (2005)
- Crawshaw LI, "Responses to rapid temperature change in vertebrate ectotherms", Amer Zool, 19, pp.225~237 (1979)
- Cuesta A, "Salinity in-fluences the humoral immune parameters of gilthead seabream (Sparus aurata L.)", Fish Shellfish Immunol, 18, pp.255~261 (2005)
- Dominguez M, "Effects of changes in environmental factors on the non-specific immune response of Nile tilapia, Oreochromis niloticus L", Aquat Res, 36, pp.391~397 (2005)
- Ellis AE, "Immunity to bacteria in fish", Fish Shellfish Immunol, 9, pp.291~308 (1999)
- Esteban MA, "Methodological aspects of assessing phagocytosis of Vibrio anguillarum by leucocytes of gilthead sea-bream (Sparus aurata L.) by flow cytometry and electron microscopy", Cell Tissue Res, 293, pp.133~141 (1998)
- Fevolden SE, "A combined salt and confinement stress enhances morta-lity in rainbow trout (Oncorhynchus mykiss) selected for high stress responsiveness", Aquaculture, 216, pp.67~76 (2003)
- Jaso-Friedmann L, "Regulation of innate immunity in tilapia: activation of nonspecific cytotoxic cells by cytokine-like factors", Dev Comp Imm, 24, pp.25~36 (2002)
- Kajimura S, "Changes in plasma concentrations of immunoreactive ouabain in the tilapia in response to changing salinity: is ouabain a hormone in fish?", Gen Comp Endocrin, 135, pp.90~99 (2004)
- Lee TH, "Isoform expression of Na+-K+- ATPase alpha-subunit in gills of the teleost Oreochromis mossambicus", Amer J Physiol, 275, pp.R926~R932 (1998)
- Liebert AM, "Effect of acute stess on osmoregulation, feed intake, IGF-I, and cortisol in yearling steelhead trout (Oncorhynchus mykiss) during seawater adaptation", Gen Comp Endocrin, 148, pp.195~202 (2006)
- Lie O, "Study on lysozyme activity in some fish species", Dis Aquat Org, 6, pp.1~5 (1989)
- Marc A, "Changes in some endocrinological and non-specific immunological parameters during seawater exposure in the brown trout", J Fish Biol, 46, pp.1065~1081 (1995)
- Prabakaran M, "Immune response in the tilapia, Oreochromis mossambicus on exposure to tannery effluent", Ecotox Envir Safety, 68, pp.372~378 (2007)
- Pulsford AL, "Effects of acute stress on the immune system of the dab (Limanda limanda)", Comp Bio-chem Physiol, 109, pp.129~139 (1994)
- Roed KH, "Genetic variation in lysozyme activity in rainbow trout (Oncorhyncus mykiss)", Aquaculture, 109, pp.237~244 (1993)
- Shugar D, "The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme", Bio-chem Biophysica Acta, 8, pp.302~309 (1952)
- Takahashi Y, "Enzymatic properties of partially purified lysozyme from the skin mucus of carp", Bull Japan Soc Sci Fisheries, 52, pp.1209~1214 (1986)
- Taylor F, "The influence of ploidy on saltwater adaptation, acute stress response and immune function following seawater transfer in non-smolting rainbow trout", Gen Comp Endocrin, 152, pp.314~325 (2007)
- Taylor SL, "Streptococcus iniae inhibition of apoptosis of nonspecific cytotoxic cells: a mechanism of activation of innate immunity in teleosts", Dis Aquat Org, 46, pp.15~21 (2001)
- Thompson I, "The effect of stress on the immune response of Atlantic salmon (Salmo salar L.) fed diets containing different amounts of vitamin C", Aquaculture, 114, pp.1~18 (1993)
- Vazzana M, "Confinement stress in sea bass (Dicentrarchus labrax) depresses peritoneal leukocyte cytotoxicity", Aquaculture, 210, pp.231~243 (2002)
- Weng CF, "Acute changes in gill Na+-K+ ATPase and creatine kinase in response to salinity changes in the euryhaline teleost, tilapia (Oreo-chromis mossambicus)", Physiol Biochem Zool, 75, pp.29~37 (2002)
- Yousif AN, "Occu-rrence of lysozyme in the eggs of coho salmon Oncorhynchus kisutch", Dis Aquat Org, 10, pp.45~49 (1991)