The dietary effect of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The study investigated the dietary effects of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus. Tilapia were divided in four groups. The first is a fish group fed a basal diet added with 40% medicinal herbs extract (MHE). The second is a fish group fed a basal diet supplied with 2×10⁸ CFU/g of 2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp, respectively (PB). The third group was fed with a mixture of probiotics (2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp) with the medicinal herbs extract added in basal diet (MHE+PB). The fourth group was fed only a basal diet (C). In a non-specific immune parameters analysis, respiratory burst activity, lysozyme activity, phagocytic activity (PA), alternative complement pathway activity (ACH50) and superoxide dismutase (SOD) activity were significantly (p<0.05) increased in the group MHE+PB compared to other groups. Both PB and MHE groups showed a significant (p<0.05) increase in respiratory burst activity, lysozyme activity compared to the control C group, whereas no significant differences were observed in PA, ACH50 and SOD activity compared to the control group. In challenging test, fish were administered with Edwardsiella tarda (E. tarda) on 30 days after feeding with each experimental diet and viable E. tarda cell reduction was checked over 21 days post injection. MHE+PB group showed a significantly (p<0.05) reduced E. tarda cells compared to other groups. No significant antibacterial difference (p>0.05) was observed between PB and MHE only treated group. Compared to the control, a significant antibacterial difference (p<0.05) appeared in PB but not in MHE (p>0.05). The results suggest that the probiotics and MHE mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
Abstract
The study investigated the dietary effects of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus. Tilapia were divided in four groups. The first is a fish group fed a basal diet added with 40% medicinal herbs extract (MHE). The second is a fish group fed a basal diet supplied with 2×10⁸ CFU/g of 2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp, respectively (PB). The third group was fed with a mixture of probiotics (2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp) with the medicinal herbs extract added in basal diet (MHE+PB). The fourth group was fed only a basal diet (C). In a non-specific immune parameters analysis, respiratory burst activity, lysozyme activity, phagocytic activity (PA), alternative complement pathway activity (ACH50) and superoxide dismutase (SOD) activity were significantly (p<0.05) increased in the group MHE+PB compared to other groups. Both PB and MHE groups showed a significant (p<0.05) increase in respiratory burst activity, lysozyme activity compared to the control C group, whereas no significant differences were observed in PA, ACH50 and SOD activity compared to the control group. In challenging test, fish were administered with Edwardsiella tarda (E. tarda) on 30 days after feeding with each experimental diet and viable E. tarda cell reduction was checked over 21 days post injection. MHE+PB group showed a significantly (p<0.05) reduced E. tarda cells compared to other groups. No significant antibacterial difference (p>0.05) was observed between PB and MHE only treated group. Compared to the control, a significant antibacterial difference (p<0.05) appeared in PB but not in MHE (p>0.05). The results suggest that the probiotics and MHE mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
참고문헌 (37)
- Aly, S. M., "Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections", Fish & Shellfish Immunol., 25, pp.128~136 (2008)
- Amderson, D. P., "Neutrophil glass-adherent, nitroblue tetrazolium assay gives early indication of immunization effectiveness in rainbow trout", Vet. Immunol Immunopathol, 30, pp.419~429 (1992)
- Bagheri, T., "Growth, survival and gut microbial load of rainbow trout (Onchorhynchus milkiss) fry given diet supplemented with probiotic during the two months of first feeding", Turkish J. of Fisheries and Aquat. Sci, 8, pp.43~48 (2008)
- Balcazar, J. L., "The role of probiotics in aquaculture", Vet. Microbiol, 114, pp.173~186 (2006)
- Boonthai, T., "Probiotics bacteria effects on growth and bacterial composition of black tiger shrimp(Penaus monodon)", Aquacul. Nutr, 9, pp.124~130 (2011)
- Chiu, C. H., "Dietary administration of the probiotics, Saccharomyces cerevisiae P13, enhanced the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides", Fish & Shefish Immunol, 29, pp.1053~1059 (2010)
- Da Silva, B.C., "Salts of organic acids selection by multiple characteristics for marine shrimp nutrition", Aquacul, 345, pp.384~387 (2013)
- Defoirdt, T., "Short-chain fatty acids protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbelli", Aquacul, 261, pp.804~808 (2006)
- Ellis A. E., "Non-specific defense mechanism in fish and their role in disease processes", Dev. Biol. Stand, 49, pp.337~352 (1981)
- Ellis, A. E., "Immunity to bacteria in fish", Fish & Shellfish Immunol, 9, pp.291~308 (1999)
- Fernandez, R., "Effect of lactic acid bacteria administered orally on growth perofrmance of Penaeus indicus (H. Milne Edwards)Juveniles", Res. J. of Microbiol, 6, pp.466~479 (2011)
- Fletcher TC, "The identification of non-specific humoral factors in the plaice (Pleuronectes platessa L.)", Dev. Biol. Stand, 49, pp.332~327 (1981)
- Food and Agriculture Organization of the United nations, "World Rev. of Fisheries and Aquacul", World Fisheries, ~ (2014)
- Food and Agriculture Organization of the United nations, "World Rev. of Fisheries and Aquacul", World Fisheries, ~ (2014)
- Harikrishnan, R., "Probiotics and herbal mixtures enhance the growth, blood constituents, and nonspecific immune response in Paralichthys olivaceus against Streptococcus parauberis", Fish & Shellfish Immunol, 31, pp.310~317 (2011)
- Hassett, D. J., "Bacterial adaptation to oxidative stress: implications for pathogenesis and interaction with phagocytic cells", Fed. Am. Soc. Exp. Biol., 3, pp.2537~2581 (1989)
- Responsible Use of Antibiotics in Aquaculture, "Hernández Serrano, P.", FAO, ~, pp.FAO Fisheries Technical Paper. No. 469 (2005)
- Inai, S., "Complementology", pp.119~140 (1982), Ishiyaku Publishers Inc.
- 전봉근, "한약재 추출물에 배양된 유산균 배양액이 양식어류의 사료첨가제로서 성장과 비 특이적 면역에 미치는 영향", 생명과학회지, 19(1), pp.87~93 (2009), 한국생명과학회
- 김진도, "Yeast β-glucan 첨가 사료가 뱀장어의 비특이적 면역 반응에 미치는 영향", 한국어병학회지, 21(3), pp.219~228 (2008), 한국어병학회
- Kishimoto, T. K., "Neutrophil MAC-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors", Science, 245, pp.1238~1241 (1985)
- Kusuda, R., "Characterization of anti-body dependent hemolytic activity in eel serum", Nip-pon Suisan Gakkaishi, 53, pp.111~121 (1987)
- Liu, C. H., "Dietary administration of the probiotic, Bacillus subtilis E20, enhances the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides", Fish Shellfish Immunol, 33, pp.699~706 (2012)
- Liu, C. H., "Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease-producing probiotics, Bacillus subtilis E20, from natto", J App. Microbial, 107, pp.1031~1041 (2009)
- Magnuson, D. K., "Human endothelial cell adhesiveness for meutrophils, induced by Escherichia coli lipopolysaccharide in vitro, is inhibited by Bacteroides fragilis lipopolysaccharide", J. Immunol, 143, pp.3024~3033 (1989)
- Ng, W.K, "Standards for Acidifiers - Principles for the Use of Organic Acids in Animal Nutrition", pp.49~58 (2011), Nottingham University Press
- Perez, T., "Host-microbiota interactions within the fish intestinal ecosystem", Mucosal Immunol., 3, pp.335~360 (2010)
- Santarem, M., "Effect of β-glucans on the non-specific immune responses of turbot (Scophthalmus maximus L.)", Fish & shellfish Immunol, 7(6), pp.429~437 (1997)
- Secombes, C. J., "Superoxide anion production by rainbow trout macrophages detected by the reduction of ferricytochrome c", Dev. Comp. Immunol, 12, pp.201~206 (1988)
- Sheikhzadeh, N., "Effects of dietary Ergosan on cutaneous mucosal immune response in rainbow trout (Oncorhynchus mykiss)", Fish Shellfish Immunol., 32, pp.407~441 (2012)
- Verschuere, L. R. G., "Probiotic bacteria as biological control agents in aquaculture", Microbiol. Mol. Biol., 64, pp.655~671 (2000)
- Wang, S.H., "The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp", Litopenaeus vannamei Fish & Shellfish Immunol, 19, pp.191~204 (2005)
- Wang, Y. B., "Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei", Aquacul, 269, pp.259~264 (2007)
- Wang, Y. B., "Effect of probiotics, Enteroccus faecium, on tilapia(Oreochromis niloticus)growth performance and immune response", Aquacul, 277, pp.203~207 (2008)
- Aquaculture: changing the face of the waters:Meeting the promise and challenge of sustainable aquaculture, "World Bank", World Bank, ~ (2006)
- Yano, T., "Tech. in fish Immunol. New Jersey", pp.131~141 (1992), SOS Publications
- Zokaeifar, H., "Effects of Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei", Fish Shellfish Immunol, 33(4), pp.683~689 (2012)