과산화수소의 농도와 적용시간이 상아질의 깊이에 따라 레진 수복물의 미세인장결합강도에 미치는 영향
THE EFFECT OF CONCENTRATION AND APPLICATION TIME OF HYDROGEN PEROXIDE ON THE MICROTENSILE BOND STRENGTH OF RESIN RESTORATIONS TO THE DENTIN AT DIFFERENT DEPTHS
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초록
본 연구의 목적은 심부 상아질과 치수실 상아질에 H2O2를 각기 다른 적용시간과 농도로 적용하였을 때, 레진수복물과의 미세인장결합강도에 미치는 영향을 평가하는 것이다. 준비된 각각의 치아에 전형적인 근관와동을 형성하고 다음과 같이 무작위로 1개의 대조군과, 4개의 실험군으로 나누었다: 1 군, 전처리를 시행하지 않았다; 2 군, 20% H2O2를 20분, 10분, 5분간 적용하였다; 3 군, 10% H2O2를 20분, 10분, 5분간 적용하였다; 4 군, 5% H2O2를 20분, 10분, 5분간 적용하였다; 5 군, 2.5% H2O2를 20분, 10분, 5분간 적용하였다. 위와 같이 처리한 후 와동 내를 Superbond C&B(Sunmedical,Co., Shiga, Japan)로 충전하였다. 각 치아는 37℃ 증류수에 24시간 저장한 뒤, 심부 상아질과 치수실 상아질로 잘라 미세인장결합강도 시험을 시행하였다. 각 군에서 측정된 미세인장결합강도를 3-way ANOVA와 Tukey post hoc test로 통계 처리하였다(p < 0.05). 실험 결과 모든 군에서 심부 상아질(D1)의 미세인장 결합강도가 치수실 상아질(D2)보다 높게 나타났다. 평균 결합강도는 H2O2의 농도와 적용 시간이 증가할수록 감소하였다. 통계분석에서 상아질의 깊이, H2O2 농도와 적용 시간이라는 세 가지 변수간의 교호작용 효과는 없으나(p > 0.05), 상아질의 깊이와 H2O2 농도 사이, H2O2 농도와 적용 시간 사이에서는 교호작용 효과가 있었다(p < 0.05). SEM 관찰에서는 H2O2의 농도가 증가할수록 상아세관이 좀 더 개방된 양상을 보였다.
Abstract
The purpose of this study was to examine the effect of hydrogen peroxide at different application time and concentrations on the microtensile bond strength of resin restorations to the deep and the pulp chamber dentin. A conventional endodontic access cavity was prepared in each tooth, and then the teeth were randomly divided into 1 control group and 4 experimental groups as follows: Group 1, non treated; Group 2, with 20% Hydrogen peroxide(H2O2); Group 3, with 10% H2O2; Group 4, with 5% H2O2; Group 5, with 2.5% H2O2; the teeth of all groups except group 1 were treated for 20, 10, and 5min. The treated teeth were filled using a Superbond C&B (Sun medical Co., Shiga, Japan). Thereafter, the specimens were stored in distilled water at 37℃ for 24-hours and then sectioned into the deep and the chamber dentin. The microtensile bond strength values of each group were analyzed by 3-way ANOVA and Tukey post hoc test(p < 0.05). In this study, the microtensile bond strength of the deep dentin (D1) was significantly greater than that of the pulp chamber dentin (D2) in the all groups tested. The average of microtensile bond strength was decreased as the concentration and the application time of H2O2 were increased. Analysis showed significant correlation effect not only between the depth of the dentin and the concentration of H2O2 but also between the concentration of H2O2 and the application time(p < 0.05), while no significant difference existed among these three variables(p > 0.05). The higher H2O2 concentration, the more opened dentinal tubules under a scanning electron microscope(SEM) examination.
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