β-glycerophosphate 혼합시 인간 치수 세포에 대한 Portland cement의 생활성에 관한 연구
A BIOACTIVITY STUDY OF PORTLAND CEMENT MIXED WITH β-GLYCEROPHOSPHATE ON HUMAN PULP CELL
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초록
β-glycerophosphate는 치수의 상아모세포 분화를 촉진하는 물질이다. Portland cement는 수중에서 장기간에 걸쳐 용해되기 때문에 β-glycerophosphate을 혼합한 Portland cement는 수산화칼슘과 함께 β-glycerophosphate를 장기 간 용출하게 된다. 본 실험에서는 β-glycerophosphate을 혼합한 Portland cament에 대한 인간치수세포의 반응을 알아보았다. 인간 치수 세포에 대한 β-glycerophosphate의 효과를 알아보기 위해 다양한 농도의 β-glycerophosphate와 dexamethasone에 대한 인간 치수 세포의 ALP activity을 측정하였고 alizarin red S로 염색하여 관찰하였다. β-glycerophosphate가 다양한 농도(10 mM, 100 mM, 1 M)로 혼합된 Portland cement에 대한 인간 치수 세포의 MTS assay, ALP activity를 측정하고 SEM으로 관찰하였다. 치수세포의 석회화 정도를 관찰한 연구에서 β-glycerophosphate와dexamethasone 단독으로 적용하였을 때 거의 효과가 없었으나 5 mM β-glycerophosphate와 100 nM dexamethasone을 혼합 적용하였을 때 가장 높은 ALP acticity를 보였다. 분화제를 첨가하거나 첨가하지 않은 모든 실험군에서 치수세포에 대한 독성은 관찰되지 않았으며 Portland cement에 10 mM β-glycerophosphate을 혼합한 시편의 ALP activity가 대조군에 비교하여 가장 많이 증가하였다. 결론적으로 β-glycerophosphate이 혼합된 Portland cement는 세포 독성이 없으며 첨가물이 없는 Portland cement에 비해 치수 분화 및 석회화를 더 많이 일으키므로 임상적으로 β-glycerophosphate을 혼합한 Portland cement 적용은 재료 하방에 더 많은 상아질을 형성시킬 것으로 추측된다.
Abstract
The purpose of this study is to investigate the response of human pulp cell on Portland cement mixed with β-glycerophosphate. To investigate the effect of β-glycerophosphate and/or dexamethasone on human pulp cell, ALP activity on various concentration of β-glycerophosphate and dexamethasone was measured and mineral nodule of human pulp cell was stained with Alizarin red S. MTS assay and ALP activity of human pulp cell on Portland cement mixed with various concentration of β-glycerophosphate (10 mM, 100mM, 1M) was measured and the specimens were examined under SEM. Addition of β-glycerophosphate or dexamethasone alone had no effect however, the addition of 5 mM β-glycerophosphate and 100 nM dexamethasone had the largest increasement in ALP activity. There was no toxicity in all samples and the data showed that Portland cement mixed with 10 mM β-glycerophosphate had more increase in ALP activity compared with control. In conclusion, Portland cement mixed with β-glycerophosphate has no toxicity and promotes differentiation and mineralization of pulp cell compared with additive-free Portland cement. This implicated that application of Portland cement mixed with β-glycerophosphate might form more reparative dentin and in turn it would bring direct pulp capping to success.
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