Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells
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Objectives: This study was performed to investigate the biocompatibility of newly introduced Bioaggregate on human pulp and PDL cells. Materials and Methods: Cells were collected from human pulp and PDL tissue of extracted premolars. Cell culture plate was coated either with Bioaggregate or white MTA, then the same number of cells were poured to cell culture dishes. Cell attachment and growth was examined under a phase microscope after 1,3 and 7 days of seeding. Cell viability was measured and the data was analyzed using Student t-test and one way ANOVA. Results: Both types of cells used in this study were well attached and grew healthy on Bioaggregate and MTA coated culture dishes. No cell inhibition zone was observed in Bioaggregate group. There was no statistical difference of viable cells between bioaggreagte and MTA groups. Conclusions: Bioaggregate appeared to be biocompatible compared with white MTA on human pulp and PDL cells. [J Kor Acad Cons Dent 2010;35(6):473-478.]
Abstract
Objectives: This study was performed to investigate the biocompatibility of newly introduced Bioaggregate on human pulp and PDL cells. Materials and Methods: Cells were collected from human pulp and PDL tissue of extracted premolars. Cell culture plate was coated either with Bioaggregate or white MTA, then the same number of cells were poured to cell culture dishes. Cell attachment and growth was examined under a phase microscope after 1,3 and 7 days of seeding. Cell viability was measured and the data was analyzed using Student t-test and one way ANOVA. Results: Both types of cells used in this study were well attached and grew healthy on Bioaggregate and MTA coated culture dishes. No cell inhibition zone was observed in Bioaggregate group. There was no statistical difference of viable cells between bioaggreagte and MTA groups. Conclusions: Bioaggregate appeared to be biocompatible compared with white MTA on human pulp and PDL cells. [J Kor Acad Cons Dent 2010;35(6):473-478.]
참고문헌 (18)
- Koh ET, "Torabinejad M, Pitt Ford TR, Brady K, McDonald F. Mineral trioxide aggregate stimulates a biological response in human osteoblasts", J Biomed Mater Res, 37(3), pp.432~439 (1997)
- Lee SJ, "Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations", J Endod, 19(11), pp.541~544 (1993)
- Torabinejad M, "Histologic assessment of mineral trioxide aggregate as a root-end filling in monkeys", J Endod, 23(4), pp.225~228 (1997)
- Gomes-Filho JE, "Evaluation of the tissue reaction to fast endodontic cement (CER) and Angelus MTA", J Endod, 35(10), pp.1377~1380 (2009)
- Hashem AA, "ProRoot MTA, MTAAngelus and IRM used to repair large furcation perforations: sealability study", J Endod, 34(1), pp.59~61 (2008)
- Song JS, "Chemical analysis of powder and set forms of Portland cement, gray ProRoot MTA, white ProRoot MTA, and gray MTA-Angelus", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 102(6), pp.809~815 (2006)
- Lessa FC, "Cytotoxic effects of White-MTA and MTA-Bio cements on odontoblast- like cells (MDPC-23)", Braz Dent J, 21(1), pp.24~31 (2010)
- Zhang H, "Dentin enhances the antibacterial effect of mineral trioxide aggregate and bioaggregate", J Endod, 35(2), pp.221~224 (2009)
- Park JW, "X-Ray diffraction analysis of white ProRoot MTA and Diadent BioAggregate", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 109(1), pp.155~158 (2010)
- Vivan RR, "Evaluation of the physical and chemical properties of two commercial and three experimental root-end filling materials", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 110(2), pp.250~256 (2010)
- Yan P, "Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts" (2010), Int Endod J In press
- Yuan Z, "Effect of bioaggregate on mineral-associated gene expression in osteoblast cells", J Endod, 36(7), pp.1145~1148 (2010)
- Chang S, "Ingredients and cytotoxicity of MTA and 3 kinds of Portland cement", J Kor Acad Cons Dent, 33(4), pp.364~376 (2008)
- 권지윤, "Mineral trioxide aggregate (MTA)가 치주인대 섬유아세포에서 분비되는 cytokine과 성장인자 TGF- β1, FGF-2 발현에 미치는 영향", 대한치과보존학회지, 32(3), pp.191~197 (2007), 대한치과보존학회
- 윤영란, "Mineral trioxide aggregate, calcium sulfate와 calcium hydroxide의 치수에 대한 반응", 대한치과보존학회지, 32(2), pp.95~101 (2007), 대한치과보존학회
- Apaydin W, "Hard-tissue healing after application of fresh or set MTA as rootend filling material", J Endod, 30(1), pp.21~24 (2004)
- Ford T, "Using mineral trioxide aggregate as a pulp-capping material", J Am Dent Assoc, 127(10), pp.1491~1494 (1996)
- De-Deus G, "Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells", J Endod, 35(10), pp.1387~1390 (2009)
