THE EFFECT OF SEVERAL ROOT-END FILLING MATERIALS ON MG63 OSTEOBLAST-LIKE CELLS
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The purpose of this study was to compare mineral trioxide aggregate (MTA; Dentsply, Tulsa Dental,Tulsa, OK, USA), which is widely used as root-end filling material, with DiaRoot BioAggregate (DB;Innovative BioCaramix Inc, Vancouver, BC, Canada), newly developed product, by using MG63 osteoblastlike cells. MTA, DB, and Intermediate Restorative Material (IRM; Dentsply Caulk, Milford, DE, USA)were used for root-end filling material while tissue culture plastic was used for control group. Each material was mixed and, the mixtures were left to set for 24 hours. MG63 cells were seeded to each group and then they were cultured for attachment for 4 hours. Following the attachment of cells to the root-end filling material, early cellular response was observed. After another 12 hours’culture, the level of attachment between cells and material was observed and in order to identify the effect of each material to bone formation,transforming growth factor beta1 (TGFβ1) and osteocalin (OC) were estimated by using enzymelinked immunosorbent assay (ELISA), and the amount of alkaline phosphatase (ALP) was also measured. The data were analyzed using one-way ANOVA. As a result, only at OC and the number of cells which were attached to materials, there was no statistical difference between MTA and DB. At other items, there was statistically significant difference in all groups. Although DB has not shown exactly the same cellular response like that of MTA, the number of attached cells shows that biocompatibility of the material and OC indicates bone formation rate. Therefore, if DB is used for root end filling material, it is expected to lead to similar results to MTA. [J Kor Acad Cons Dent 35(3):222-228, 2010]
Abstract
The purpose of this study was to compare mineral trioxide aggregate (MTA; Dentsply, Tulsa Dental,Tulsa, OK, USA), which is widely used as root-end filling material, with DiaRoot BioAggregate (DB;Innovative BioCaramix Inc, Vancouver, BC, Canada), newly developed product, by using MG63 osteoblastlike cells. MTA, DB, and Intermediate Restorative Material (IRM; Dentsply Caulk, Milford, DE, USA)were used for root-end filling material while tissue culture plastic was used for control group. Each material was mixed and, the mixtures were left to set for 24 hours. MG63 cells were seeded to each group and then they were cultured for attachment for 4 hours. Following the attachment of cells to the root-end filling material, early cellular response was observed. After another 12 hours’culture, the level of attachment between cells and material was observed and in order to identify the effect of each material to bone formation,transforming growth factor beta1 (TGFβ1) and osteocalin (OC) were estimated by using enzymelinked immunosorbent assay (ELISA), and the amount of alkaline phosphatase (ALP) was also measured. The data were analyzed using one-way ANOVA. As a result, only at OC and the number of cells which were attached to materials, there was no statistical difference between MTA and DB. At other items, there was statistically significant difference in all groups. Although DB has not shown exactly the same cellular response like that of MTA, the number of attached cells shows that biocompatibility of the material and OC indicates bone formation rate. Therefore, if DB is used for root end filling material, it is expected to lead to similar results to MTA. [J Kor Acad Cons Dent 35(3):222-228, 2010]
참고문헌 (24)
- Torabinejad M, "Clinical applications of mineral trioxide ag gregate", J Endod, 25, pp.197~205 (1999)
- 윤영란, "Mineral trioxide aggregate, calcium sulfate와 calcium hydroxide의 치수에 대한 반응", 대한치과보존학회지, 32(2), pp.95~101 (2007), 대한치과보존학회
- Schmitt D, "Multifaceted use of ProRoot MTA root canal repair material", Pediatr Dent, 23, pp.326~330 (2001)
- Koh ET, "Mineral trioxide aggregate stimulates a biological response in human osteoblasts", J Biomed Mater Res, 37, pp.432~439 (1997)
- Koh ET, "Cellular response to mineral trioxide aggregate", J Endod, 24, pp.543~547 (1998)
- Mitchell PJC, "Osteoblast biocompatibility of mineral trioxide aggregate", Biomater, 20, pp.167~173 (1999)
- Rausch-fan X, "Differentiation and cytokine synthesis of human alveolar osteoblasts compared to osteoblast-like cells(MG63) in response to titanium surfaces", Dent Mater, 24, pp.102~110 (2008)
- Qu Z, "The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification", J Biomed Mater Res, 82, pp.658~668 (2007)
- Perez AL, "Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot MTA and white MTA", Int Endod J, 36, pp.564~570 (2003)
- Schmalz G, "Use of cell cultures for toxicity testing of dental materials - advantages and limitations", J Dent, 22, pp.6~11 (1994)
- 강민경, "COMPARISON OF BIOCOMPATIBILITY OF FOUR ROOT PERFORATION REPAIR MATERIALS", 대한치과보존학회지, 34(3), pp.192~198 (2009), 대한치과보존학회
- Calvo MS, "Molecular basis and clinical application of biological markers of bone turnover", Endocr Rev, 17, pp.333~368 (1996)
- Kassem M, "Production and action of transforming growth factor-βin human osteoblast cultures: dependence on cell differentiation and modulation by calcitriol", Eur J Clin Invest, 30, pp.429~437 (2000)
- Diadent group international, "Material safety data sheet DiaRoot bioAggregate", ~
- De-Deus G, "Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells", J Endod, 35, pp.1387~1390 (2009)
- Zhang H, "Dentin enhances the antibacterial effect of mineral trioxide aggregate and bioaggregate", J Endod, 35, pp.221~224 (2009)
- Apaydin ES, "Hard-tissue healing after application of fresh or set MTA as rootend- filling material", J Endod, 30, pp.21~24 (2003)
- AL-Rabeah E, "Human alveolar bone cells interact with ProRoot and tooth-colored MTA", J Endod, 32, pp.872~875 (2006)
- Zhu Q, "Adhesion of human osteoblasts on root-end filling materials", J Endod, 26, pp.404~406 (2000)
- Bender MedSystems GmbH, "BMS2020INST human osteocalcin", ~ (2006)
- Thompson TS, "Cementoblasts maintain expression of osteocalcin in the presence of mineral trioxide aggregate", J Endod, 29, pp.407~412 (2003)
- Cassidy N, "Comparative analysis of transforming growth factor-β isoforms 1-3 in human and rabbit dentine matrices", Archs oral Biol, 42, pp.219~223 (1997)
- Sakai R, "Involvement of activin in the regulation of bone metabolism", Mol Cell Endocrinol, 180, pp.183~188 (2001)
- 권지윤, "Mineral trioxide aggregate (MTA)가 치주인대 섬유아세포에서 분비되는 cytokine과 성장인자 TGF- β1, FGF-2 발현에 미치는 영향", 대한치과보존학회지, 32(3), pp.191~197 (2007), 대한치과보존학회