Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell
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Objectives: The purpose of this study was to evaluate in vitro cytotoxicity of thepozzolan cement and other root-end filling materials using human periodontal ligamentcell. Materials and Methods: Endocem (Maruchi), white ProRoot MTA (Dentsply), whiteAngelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completelyin an incubator at 37℃ for 7 days, Endocem was tested in two ways: 1) immediatelyafter mixing (fresh specimens) and 2) after setting completely like other experimentalmaterials. The methods for assessment included light microscopic examination, cellcounting and WST-1 assay on human periodontal ligament cell. Results: In the resultsof microscopic examination and cell counting, Super EBA showed significantly lowerviable cell than any other groups (p < 0.05). As the results of WST-1 assay, comparedwith untreated control group, there was no significant cell viability of the Endocemgroup. However, the fresh mixed Endocem group had significantly less cell viability. Thecells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereasthe cells exposed to Super EBA displayed the lowest viability (p < 0.05). Conclusions:The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRootMTA and Angelus MTA. Considering the difficult manipulation and long setting time ofProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.
Abstract
Objectives: The purpose of this study was to evaluate in vitro cytotoxicity of thepozzolan cement and other root-end filling materials using human periodontal ligamentcell. Materials and Methods: Endocem (Maruchi), white ProRoot MTA (Dentsply), whiteAngelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completelyin an incubator at 37℃ for 7 days, Endocem was tested in two ways: 1) immediatelyafter mixing (fresh specimens) and 2) after setting completely like other experimentalmaterials. The methods for assessment included light microscopic examination, cellcounting and WST-1 assay on human periodontal ligament cell. Results: In the resultsof microscopic examination and cell counting, Super EBA showed significantly lowerviable cell than any other groups (p < 0.05). As the results of WST-1 assay, comparedwith untreated control group, there was no significant cell viability of the Endocemgroup. However, the fresh mixed Endocem group had significantly less cell viability. Thecells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereasthe cells exposed to Super EBA displayed the lowest viability (p < 0.05). Conclusions:The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRootMTA and Angelus MTA. Considering the difficult manipulation and long setting time ofProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.
참고문헌 (31)
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