본문 바로가기
KERD
논문KCI 등재

Effects of four novel root-end filling materials on the viability of periodontal ligament fibroblasts

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류치의학

초록

Objectives: The aim of this in vitro study was to evaluate the biocompatibility of newly proposed root-end filling materials, Biodentine, Micro-Mega mineral trioxide aggregate (MM-MTA), polymethylmethacrylate (PMMA) bone cement, and Smart Dentin Replacement (SDR), in comparison with contemporary root-end filling materials, intermediate restorative material (IRM), Dyract compomer, ProRoot MTA (PMTA), and Vitrebond, using human periodontal ligament (hPDL) fibroblasts. Materials and Methods: Ten discs from each material were fabricated in sterile Teflon molds and 24-hour eluates were obtained from each root-end filling material in cell culture media after 1- or 3-day setting. hPDL fibroblasts were plated at a density of 5 × 103 /well, and were incubated for 24 hours with 1:1, 1:2, 1:4, and 1:8 dilutions of eluates. Cell viability was evaluated by XTT assay. Data was statistically analysed. Apoptotic/necrotic activity of PDL cells exposed to material eluates was established by flow cytometry. Results: The Vitrebond and IRM were significantly more cytotoxic than the other root-end filling materials (p < 0.05). Those cells exposed to the Biodentine and Dyract compomer eluates showed the highest survival rates (p < 0.05), while the PMTA, MM-MTA, SDR, and PMMA groups exhibited similar cell viabilities. Three-day samples were more cytotoxic than 1-day samples (p < 0.05). Eluates from the cements at 1:1 dilution were significantly more cytotoxic (p < 0.05). Vitrebond induced cell necrosis as indicated by flow cytometry. Conclusions: This in vitro study demonstrated that Biodentine and Compomer were more biocompatible than the other root-end filling materials. Vitrebond eluate caused necrotic cell death.

Abstract

Objectives: The aim of this in vitro study was to evaluate the biocompatibility of newly proposed root-end filling materials, Biodentine, Micro-Mega mineral trioxide aggregate (MM-MTA), polymethylmethacrylate (PMMA) bone cement, and Smart Dentin Replacement (SDR), in comparison with contemporary root-end filling materials, intermediate restorative material (IRM), Dyract compomer, ProRoot MTA (PMTA), and Vitrebond, using human periodontal ligament (hPDL) fibroblasts. Materials and Methods: Ten discs from each material were fabricated in sterile Teflon molds and 24-hour eluates were obtained from each root-end filling material in cell culture media after 1- or 3-day setting. hPDL fibroblasts were plated at a density of 5 × 103 /well, and were incubated for 24 hours with 1:1, 1:2, 1:4, and 1:8 dilutions of eluates. Cell viability was evaluated by XTT assay. Data was statistically analysed. Apoptotic/necrotic activity of PDL cells exposed to material eluates was established by flow cytometry. Results: The Vitrebond and IRM were significantly more cytotoxic than the other root-end filling materials (p < 0.05). Those cells exposed to the Biodentine and Dyract compomer eluates showed the highest survival rates (p < 0.05), while the PMTA, MM-MTA, SDR, and PMMA groups exhibited similar cell viabilities. Three-day samples were more cytotoxic than 1-day samples (p < 0.05). Eluates from the cements at 1:1 dilution were significantly more cytotoxic (p < 0.05). Vitrebond induced cell necrosis as indicated by flow cytometry. Conclusions: This in vitro study demonstrated that Biodentine and Compomer were more biocompatible than the other root-end filling materials. Vitrebond eluate caused necrotic cell death.

참고문헌 (36)

  1. Kim S, "Modern endodontic surgery concepts and practice: a review", J Endod, 32, pp.601~623 (2006)[DOI]
  2. Chong BS, "A prospective clinical study of Mineral Trioxide Aggregate and IRM when used as root-end filling materials in endodontic surgery", Int Endod J, 36, pp.520~526 (2003)[DOI]
  3. Ma J, "Biocompatibility of two novel root repair materials", J Endod, 37, pp.793~798 (2011)[DOI]
  4. Chong BS, "Short-term tissue response to potential root-end filling materials in infected root canals", Int Endod J, 30, pp.240~249 (1997)[DOI]
  5. Mazumdar D, "An investigation into the irritant properties of some retrograde filling materials-an in vivo study", J Conserv Dent, 8, pp.4~13 (2005)
  6. McDonald NJ, "A comparative retrofill leakage study utilizing a dentin bonding material", J Endod, 13, pp.224~227 (1987)[DOI]
  7. Dragoo MR. Resin-ionomer, "Human clinical and histologic wound healing responses in specific periodontal lesions", Int J Periodontics Restorative Dent, 17, pp.75~87 (1997)
  8. Stanczyk M, "Thermal analysis of bone cement polymerisation at the cement-bone interface", J Biomech, 37, pp.1803~1810 (2004)[DOI]
  9. Badr AE, "Marginal adaptation and cytotoxicity of bone cement compared with amalgam and mineral trioxide aggregate as root-end filling materials", J Endod, 36, pp.1056~1060 (2010)[DOI]
  10. Torabinejad M, "Investigation of mineral trioxide aggregate for root-end filling in dogs", J Endod, 21, pp.603~608 (1995)[DOI]
  11. Corral Nunez CM, "Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line", J Endod, 40, pp.406~411 (2014)[DOI]
  12. Zhou HM, "In vitro cytotoxicity evaluation of a novel root repair material", J Endod, 39, pp.478~483 (2013)[DOI]
  13. International Organization for Standardization, "ISO 10993-5 Biological evaluation of medical devices - Part 5: tests for in vitro cytotoxicity. 3rd ed", International Organization for Standardization, ~ (2009)
  14. Scudiero DA, "Evaluation of a soluble tetrazolium", formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines, 1988, pp.48, 4827~4833
  15. Haglund R, "Effects of root-end filling materials on fibroblasts and macrophages in vitro", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 95, pp.739~745 (2003)[DOI]
  16. Sousa CJ, "A comparative histological evaluation of the biocompatibility of materials used in apical surgery", Int Endod J, 37, pp.738~748 (2004)[DOI]
  17. Nicholson JW, "The biocompatibility of resin-modified glass-ionomer cements for dentistry", Dent Mater, 24, pp.1702~1708 (2008)[DOI]
  18. Savarino L, "In vitro investigation of aluminum and fluoride release from compomers, conventional and resin-modified glass-ionomer cements: a standardized approach", J Biomater Sci Polym Ed, 11, pp.289~300 (2000)[DOI]
  19. Camargo SE, "Cytotoxicity and genotoxicity of pulp capping materials in two cell lines", Int Endod J, 42, pp.227~237 (2009)[DOI]
  20. Rodriguez IA, "An in vitro biocompatibility study of conventional and resin-modified glass ionomer cements", J Adhes Dent, 15, pp.541~546 (2013)
  21. Al-Hiyasat AS, "Cytotoxicity evaluation of dental resin composites and their flowable derivatives", Clin Oral Investig, 9, pp.21~25 (2005)[DOI]
  22. Geurtsen W, "Residual monomer", additive release and variability in cytotoxicity of light-curing glass-ionomer cements and compomers, 1998, pp.77, 2012~2019
  23. Tunc ES, "Cytotoxic effects of halogen- and light-emitting diode-cured compomers on human pulp fibroblasts", Int J Paediatr Dent, 19, pp.55~60 (2009)[DOI]
  24. Stafford GD, "The loss of residual monomer from acrylic orthodontic resins", Dent Mater, 1, pp.135~138 (1985)[DOI]
  25. Schembri M, "Analyses of heavy metals in mineral trioxide aggregate and Portland cement", J Endod, 36, pp.1210~1215 (2010)[DOI]
  26. Chang SW, "Effects of ProRoot MTA, Bioaggregate, and Micromega MTA on odontoblastic differentiation in human dental pulp cells", J Endod, 40, pp.113~118 (2014)[DOI]
  27. Kum KY, "Trace metal contents of three tricalcium silicate materials: MTA Angelus, Micro Mega MTA and Bioaggregate", Int Endod J, 47, pp.704~710 (2014)[DOI]
  28. Gandolfi MG, "New Portland cement-based materials for endodontics mixed with articaine solution: a study of cellular response", J Endod, 34, pp.39~44 (2008)
  29. Camilleri J, "Biocompatibility of two commercial forms of mineral trioxide aggregate", Int Endod J, 37, pp.699~704 (2004)[DOI]
  30. Dion I, "Physico-chemistry and cytotoxicity of ceramics: part I: characterization of ceramic powders", J Mater Sci Mater Med, 8, pp.325~332 (1997)[DOI]
  31. Strassler HE, "Biodentine tricalcium-silicate cement", Inside Dent, 7, pp.98~100 (2011)
  32. Camilleri J, "Characterization and analyses of acid-extractable and leached trace elements in dental cements", Int Endod J, 45, pp.737~743 (2012)[DOI]
  33. 장영은, "Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials", Restorative Dentistry and Endodontics, 39(2), pp.89~94 (2014), 대한치과보존학회[DOI]
  34. Markowitz K, "Biologic properties of eugenol and zinc oxide-eugenol. A clinically oriented review", Oral Surg Oral Med Oral Pathol, 73, pp.729~737 (1992)[DOI]
  35. Uzun IH, "In vitro evaluation of long-term cytotoxic response of injection-molded polyamide and polymethyle metacrylate denture base materials on primary fibroblast cell culture", Acta Odontol Scand, 71, pp.1267~1272 (2013)[DOI]
  36. Wei W, "Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells", J Endod, 38, pp.936~942 (2012)[DOI]

관련 논문AI추천

Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell김수연, 김의성, 송민주, 윤태선대한치과보존학회 · Restorative Dentistry and Endodontics2014 · 제39권 제1호In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assayFarimah Masoumi, Jamshid Hadjati, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer, Sedigheh Khedmat, Somayyeh Dehghan대한치과보존학회 · Restorative Dentistry and Endodontics2014 · 제39권 제3호Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials고정태, 박영준, 오원만, 이빈나, 장영은, 장훈상, 주남억, 황윤찬, 황인남대한치과보존학회 · Restorative Dentistry and Endodontics2014 · 제39권 제2호Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells김의성, 신수정, 정주령대한치과보존학회 · Restorative Dentistry and Endodontics2010 · 제35권 제6호COMPARISON OF BIOCOMPATIBILITY OF FOUR ROOT PERFORATION REPAIR MATERIALS강민경, 고정태, 배인호, 오원만, 황윤찬, 황인남대한치과보존학회 · Restorative Dentistry and Endodontics2009 · 제34권 제3호Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials김성오, 송제선, 신유석, 이제호, 이혜원, 정재은대한소아치과학회 · 대한소아치과학회지2015 · 제42권 제4호

이 간행물의 다른 논문 (10건)