Novel Calcium Phosphate Glass for Hard-Tissue Regeneration
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: The aim of this review is to introduce a novel bone-graft material for hard-tissue regeneration based on the calcium phosphate glass(CPG). Materials and Methods: CPG was synthesized by melting and subsequent quenching process in the system of CaO-CaF2-P2O5-MgO-ZnO having a much lower Ca/P ratio than that of conventional calcium phosphates such as HA or TCP. The biodegradability and bioactivity were performed. Effects on the proliferation, calcification and mineralization of osteoblast-like cells were examined in vitro. Influence in new bone and cementum formations was investigated in vivo using calvarial defects of Sprague-Dawley rats as well as 1-wall intrabony defect of beagle dogs. The application to the tissue-engineered macroporous scaffold and in vitro and in vivo tests was explored. Results: The extent of dissolution decreased with increasing Ca/P ratio. Exposure to either simulated body fluid or fetal bovine serum caused precipitation on the surface. The calcification and mineralization of osteoblast-like cells were enhanced by CPG. CPG promoted new bone and cementum formation in the calvarial defect of Sprague-Dawley rats after 8 weeks. The macroporous scaffolds can be fabricated with 500~800μm of pore size and a three-dimensionally interconnected open pore system. The stem cells were seeded continuously proliferated in CPG scaffold. Extracellular matrix and the osteocalcin were observed at the 2nd days and 4th week. A significant difference in new bone and cementum formations was observed in vivo (p＜0.05). Conclusion: The novel calcium phosphate glass may play an integral role as potential biomaterial for regeneration of new bone and cementum. (J Korean Acad Periodontol 2008;38:273-298)
Abstract
Purpose: The aim of this review is to introduce a novel bone-graft material for hard-tissue regeneration based on the calcium phosphate glass(CPG). Materials and Methods: CPG was synthesized by melting and subsequent quenching process in the system of CaO-CaF2-P2O5-MgO-ZnO having a much lower Ca/P ratio than that of conventional calcium phosphates such as HA or TCP. The biodegradability and bioactivity were performed. Effects on the proliferation, calcification and mineralization of osteoblast-like cells were examined in vitro. Influence in new bone and cementum formations was investigated in vivo using calvarial defects of Sprague-Dawley rats as well as 1-wall intrabony defect of beagle dogs. The application to the tissue-engineered macroporous scaffold and in vitro and in vivo tests was explored. Results: The extent of dissolution decreased with increasing Ca/P ratio. Exposure to either simulated body fluid or fetal bovine serum caused precipitation on the surface. The calcification and mineralization of osteoblast-like cells were enhanced by CPG. CPG promoted new bone and cementum formation in the calvarial defect of Sprague-Dawley rats after 8 weeks. The macroporous scaffolds can be fabricated with 500~800μm of pore size and a three-dimensionally interconnected open pore system. The stem cells were seeded continuously proliferated in CPG scaffold. Extracellular matrix and the osteocalcin were observed at the 2nd days and 4th week. A significant difference in new bone and cementum formations was observed in vivo (p＜0.05). Conclusion: The novel calcium phosphate glass may play an integral role as potential biomaterial for regeneration of new bone and cementum. (J Korean Acad Periodontol 2008;38:273-298)
참고문헌 (45)
- Brow RK, "Review: the structure of simple phosphate glasses", J Non-Cryst Solids, 263-264, pp.1~28 (2000)
- Gao H, "Effect of composition on the release kinetics of phosphate controlled release glasses in aqueous medium", J Control Release, 96, pp.21~28 (2004)
- Franks K, "Development of soluble glasses for biomedical use. Part I: In vitro solubility measurement", J Mater Sci-Mater Med, 11, pp.609~614 (2000)
- Dias AG, "In vitro degradation studies of calcium phosphate glass ceramics prepared by controlled crystallization", J Non-Cryst Solids, 330, pp.81~89 (2003)
- Hosono H, "Porous glass-ceramics composed of a titanium phosphate crystal skeleton: a review", J Non-Crystal Solids, 190, pp.185~197 (1995)
- Knowles JC, "Investigation of the solubility and ion release in the glass system K2O-Na2O -CaO-P2O5", Biomaterials, 22, pp.3091~3096 (2001)
- LeGeros RZ, "Synthesis of amorphous calcium phosphates for hard tissue repair using conventional melting technique", J Mater Sci, 39, pp.5577~5579 (2004)
- Dayanand C, "Structural investigations of phosphate glasses: a detailed infared study of the x(PbO)-(1-x)P2O5 vitreous system", J Mater Sci, 31, pp.1945~1967 (1996)
- Hedgens JJ, "Glass transition and infrared spectra of low-alkali, anhydrous lithium phosphate glasses", J Am Ceram Soc, 76, pp.1691~1696 (1993)
- Khafagy AH, "Infrared spectra and composition dependence investigations of the vitreous V2O5", P2O5 system, 1992, pp.27, 1435~1439
- LeGeros RZ, "Biodegradation and bioresorption of calcium phosphate ceramics", Clin Mater, 14, pp.65~88 (1993)
- Lee YK, "Proliferation, differentiation and calcification of preosteoblast-like MC3T3-E1 cells cultured onto non-crystalline calcium phosphate glass", J Biomed Mater Res, 69A, pp.188~195 (2004)
- Sudo H, "In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria", J Cell Biol, 96, pp.191~198 (1983)
- Quarles LD, "Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: An in vitro model of osteoblast development", J Bone Mineral Res, 7, pp.683~692 (1992)
- Hunter A, "Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopedic use", Biomaterials, 16, pp.287~295 (1995)
- Owen TA, "Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationship in expression of genes associated with osteoblast proliferation and differentiation during formation of bone extracellular matrix", J Cell Physiol, 143, pp.420~430 (1990)
- Lowry OH, "The quantitative histochemistry of brain", J Biol Chem, 207, pp.19~37 (1954)
- Towbin H, "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications", Proc Natl Acad Sci USA, 9, pp.4350~4354 (1979)
- Gerstenfeld LC, "Expression of differentiated function by mineralizing cultures of chicken osteoblasts", Dev Biol, 122, pp.49~60 (1987)
- Siffert RS, "The role of ALPase in osteogenesis", J Exp Med, 93, pp.415~425 (1951)
- El-Ghannam A, "Porous bioactive glass and hydroxyapatite ceramic affect bone cell function in vitro along different time lines", J Biomed Mater Res, 36, pp.167~180 (1997)
- Itakura Y, "Development of a new system for evaluating the biocompatibility of implant materials using an osteogenic cell line(MC3T3-E1)", J. Biomed Mater Res, 22, pp.613~622 (1988)
- McGee-Russel SM, "Histochemical methods for calcium", J Histochem Cytochem, 6, pp.22~42 (1958)
- Nakamura T, "A new glass-ceramic for bone replacement: Evaluation of its bonding to bone tissue", J Biomed Mater Res, 19, pp.685~698 (1985)
- de Bruijin JD, "van Blitterswijk CA. The ultrastructure of the bone-hydroxyapatite interface in vitro", J Biomed Mater Res, 26, pp.1365~1382 (1992)
- Moon HJ, "Bone formation in calvarial defects of Sprague-Dawley rats by transplantation of calcium phosphate glass", J Biomed Mater Res, 74A, pp.497~502 (2005)
- Freeman E, "The role of osseous coagulum as a graft material", J Perio Res, 8, pp.229~235 (1973)
- Tagaki K, "The reaction of dura to bone morphogenic protein(BMP) in repair of skull defects", Ann Surg, 196, pp.100~102 (1982)
- Schmitz JP, "The critical size defect as an experimental model for craniomandibulofacial nonunions", Clin Orthop Rel Res, 205, pp.299~308 (1986)
- Marden LJ, "Recombinant human bone morphogenetic protein-2 is superior to demineralized bond matrix in reparting craniotomy defects in rats", J Biomed Mater Res, 28, pp.1127~1138 (1994)
- Bosch C, "Importance of the critical-sized bone defect in testing bone-regenerating materials", J Craniofac Surg, 9, pp.310~316 (1998)
- Schmitz JP, "Characterization of rat calvarial non-union defects", Acta Anat, 138, pp.185~192 (1990)
- Kamakura S, "Implantation of octacalcium phosphate(OCP) in rat skull defects enhances bone repair", J Dent Res, 78, pp.1682~1687 (1999)
- Park YS, "Feasibility of three-dimensional macroporous scaffold using calcium phosphate glass and polymeric sponge", J Mater Sci, 41, pp.4357~4364 (2006)
- Brown DD, "Investigation of strut crack formation in open cell alumina ceramics", J Am Ceram Soc, 77, pp.1467~1472 (1994)
- Lange FF, "Open-cell, low-density ceramics fabricate from reticulated polymer substrates", Adv Ceram Mater, 2, pp.827~831 (1987)
- Brenzy R, "Factors controlling the fracture resistance of brittle cellular materials", J Am Ceram Soc, 74, pp.1061~1065 (1991)
- Brenzy R, "Fracture behavior of open-cell ceramics", J Am Ceram Soc, 72, pp.1145~1152 (1989)
- Kim MC, "Application of X-ray micro-computed tomography on macroporous calcium phosphate glass scaffolds", Key Eng Mater, 309-311, pp.1087~1090 (2006)
- Kim J, "In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold", Key Eng Mater, 284-286, pp.679~682 (2005)
- Min DH, "Effect of calcium phosphate glass scaffold with chitosan membrane on the healing of alveolar bone in 1-wall intrabony defect in beagle dogs", Key Eng Mater, 284-286, pp.851-854~ (2005)
- Ray RD, "Bone grafts and bone implants", Otolaryngol Clin North Am, 5, pp.389~398 (1972)
- Daculsi G, "Transformation of biphasic calcium phosphate ceramics in vivo: ultrastructural and physicochemical characterization", J Biomed Mater Res, 23, pp.883~894 (1989)
- Nery EB, "Biphasic calcium phosphate ceramic combined with fabrillar collagen with and without citric acid conditioning in the treatment of periodontal osseous defects", J Periodont, 61, pp.166~172 (1990)
- Nery EB, "Biphasic calcium phosphate ceramic combined with fabrillar collagen with and without citric acid conditioning in the treatment of periodontal osseous defects", J Periodont, 63, pp.729~735 (1992)